Method for measuring biogenic amine in fresh chilled beef

A determination method and biogenic amine technology, applied in the field of chemical analysis, can solve the problems of complicated pretreatment, time-consuming derivatization, etc., and achieve the effects of simple and feasible method, less time, and simple and accurate detection method.

Inactive Publication Date: 2012-11-21
SHANGHAI OCEAN UNIV
View PDF4 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a method for the determination of biogenic amines in fresh and chilled beef,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring biogenic amine in fresh chilled beef
  • Method for measuring biogenic amine in fresh chilled beef
  • Method for measuring biogenic amine in fresh chilled beef

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0029] Example 1 Pretreatment

[0030] (1) Extraction: Weigh 5g of sample and mix with 20ml trichloroacetic acid aqueous solution (5wt%), homogenize for 3min (10000r / min), centrifuge for 5min (5000r / min), collect the supernatant and place it in a 50ml volumetric flask; filter residue Mix with 20ml of trichloroacetic acid (5%), sonicate for 10min, centrifuge for 5min, combine the supernatant into a 50ml volumetric flask, and dilute with trichloroacetic acid. Take 20ml of the supernatant and mix with 20ml of n-hexane and let it stand for 3 minutes. The lower layer is the sample layer.

[0031] In view of the coarser characteristics of beef fiber, the selection of homogenized extraction method is conducive to the dissolution of biogenic amines in beef. The use of ultrasonic secondary extraction makes the dissolution of biogenic amines more thorough and reduces the loss of biogenic amines in pretreatment. Beef is rich in fat, which has a greater impact on the recovery rate. The presen...

Example Embodiment

[0044] Feasibility analysis of the method of Example 2

[0045] (1) Standard curve and detection limit

[0046] A linear test was performed with a mixed standard solution of six kinds of biogenic amines. In each single group, the concentration of each biogenic amine was 0, 0.1, 0.3, 0.5, 1.0 mg / L. Measure according to this method, use the peak area (Y) of the quantitative ion and the corresponding standard concentration (X) as the standard curve, calculate the regression equation and correlation coefficient of the standard curve, and calculate the detection limit with S / N=3 ( table 3).

[0047] Table 36 Linear relationships and detection limits of biogenic amines

[0048]

[0049] In the linear range of 0-1.0mg / L, the correlation coefficients of the linear equations of the six biogenic amines were all greater than 0.994, and the detection limit was 10μg / kg, which met the determination requirements.

[0050] (2) Accuracy and precision

[0051] The mixed standard solution was added to th...

Example Embodiment

[0056] Example 3 Determination of beef samples

[0057] The above method was used to determine the biogenic amines in beef stored at room temperature (4°C) for 7 days. The results are shown in Table 5.

[0058] Table 5 Biogenic amine content in beef (mg / kg)

[0059]

[0060] After the beef was stored at room temperature (4°C) for 7 days, tyramine and phenethylamine were detected at 3.18mg / kg and 0.089mg / kg, respectively. The other four biogenic amines were basically not detected.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of analytical chemistry, and discloses a method for measuring biogenic amine in fresh chilled beef. After the beef is extracted by trichloroacetic acid and purified by a hybrid type cationic exchange solid phase extraction column, a high efficient liquid chromatography-tandem mass spectrometer method is adopted to simultaneously measure six common biogenic amines (cadaverine, tyramine, tryptamine, putrescine, histamine, phenylethyamine) in the beef. The chromatogram part adopts a chromatographic column with hydrophilic function to perform water-methyl cyanide gradient elution; and the mass spectrum part adopts an electro-spray ion source cation model, a multi-reaction monitoring model and an external standard method to perform mass spectrum quantitative analysis. The method requires only 15min analysis time, has good linearity within the scope of 0-1.0mg /L (r>0.994), 10Mug/kg of detection lower limit, 82.4%-116.7% of average recovery rate, and 5.3%-8.7% of standard deviation. The method is simple, convenient, quick and accurate to operate, and provides a convenient and quick method for the measurement of the biogenic amines in the fresh chilled beef.

Description

technical field [0001] The invention belongs to the field of chemical analysis, in particular to a method for determining six common biogenic amines in fresh and chilled beef. Background technique [0002] Beef is rich in nutrients and is one of the most popular meat products. Its high nutrition and high moisture characteristics make it an extremely perishable food. The growth and reproduction of microorganisms is the main cause of spoilage, and the spoilage products are mainly biogenic amines. [0003] Biogenic amines are a general term for a class of low-molecular nitrogen-containing organic compounds formed by decarboxylation of amino acids or amination of aldehydes and ketones, including aliphatic (putrescine, cadaverine, spermine, spermidine, etc.), aromatic (tyramine, phenethylamine, etc.) and heterocyclic groups (histamine, tryptamine, etc.). The chemical structures of eight common biogenic amines are shown below. [0004] [0005] Trace biogenic amines are norm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/88G01N30/06
Inventor 欧杰徐晓瑾王婧
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap