Bacillus cereus and application thereof for promoting growth of liquidambar formosana
A technology of Bacillus cereus and sweetgum, applied in the fields of biological fertilizers and microbial fertilizers, can solve problems such as no reports of Chinese sweetgum organophosphate-resolving bacteria, different types and quantities of organophosphate-resolving bacteria, etc., and achieve excellent strain resources, Significant difference, good degradation effect
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Embodiment 1
[0022] Example 1: Determination of Phosphorus Solubilizing Ability of FXY-JX21 Plate.
[0023] Egg yolk medium: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, distilled water 1000mL, agar 15.0~18.0g, pH 7.0, sterilized, when the temperature drops to about 50℃, add 3~4mL to every 50mL Fresh egg yolk liquid (equal volume of egg yolk mixed with sterile saline), mix well, pour plate.
[0024] Spot the FXY-JX21 strain activated twice on the egg yolk plate with a sterilized toothpick, and after culturing at 30°C for 3 days, measure the diameter of the phosphorus-dissolving circle and the diameter of the colony, and calculate the ratio of the phosphorus-dissolving circle to the diameter of the colony ( figure 1 ).
[0025] from figure 1 It can be seen from the figure that after the FXY-JX21 strain was cultured on the egg yolk plate, it could produce obviously opaque phosphate-dissolving circles. After measurement and calculation, the diameter of the phosphorus-dissolving ...
Embodiment 2
[0026] Example 2: Determination of the phosphorus-dissolving ability of FXY-JX21 liquid.
[0027] Montina medium: glucose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate heptahydrate 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate tetrahydrate 0.03g, eggs Phospholipid 0.4g, calcium carbonate 5.0g, distilled water 1000mL, pH 7.0
[0028]The FXY-JX21 strain activated twice was inoculated into NA medium (3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 1000mL distilled water, 18.0g agar, pH 7.2~7.4), and cultured at 30°C for 2 days, no Rinse the cells with bacterial water to make a bacterial suspension, put 1mL of the bacterial suspension into a 100mL Erlenmeyer flask containing 50mL of Montina medium, and take Montina medium with 1mL of sterile suspension as the control (CK). Each treatment was repeated 3 times, 30°C, 200r / min shaking culture for 72h, the fermentation broth was centrifuged at 4°C, 10000r / min for ...
Embodiment 3
[0030] Embodiment 3: FXY-JX21 greenhouse pot experiment.
[0031] After activating FXY-JX21 for 2~3 times, use an inoculation loop to pick a small amount of bacteria and inoculate it into a 100mL Erlenmeyer flask containing 50mL NB medium (3g beef extract, 10g peptone, 5g sodium chloride, 1000mL distilled water, pH 7.2~7.4) medium, 30°C, 200r / min shaking culture for 48h. Centrifuge the fermentation broth (4°C, 6000r / min) for 5min, rinse the bacteria with sterile normal saline for 3 times, adjust the bacterial suspension with sterile normal saline (10 8 cfu / mL) to make bacterial agent. Inoculate sweetgum Chinese sweetgum (seedling age 60 days), with the same amount of sterile saline as the control, the inoculation volume is 5mL / plant. 8 replicates per treatment were placed in the greenhouse for unified management, the light was 12h / day, and watering was timely.
[0032] The effect of FXY-JX21 strain inoculated for 90 days on the growth of annual Chinese sweetgum seedlings (s...
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