Method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling
A technology of fenugreek gum and coupling method, which is applied in the field of double-enzyme coupling extraction of fenugreek gum, can solve the problems of changes in biological activity of natural products, time-consuming and labor-intensive extraction with hot water, and low extraction rate, so as to shorten the time , mild reaction conditions and simple process
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Embodiment 1
[0018] 1) Weigh 200g of dried fenugreek seeds, crush them in a pulverizer, pass through a 200-mesh sieve, add them into 10000mL of distilled water, stir to dissolve them fully; add 0.4g of cellulase, adjust the pH to 4.5, and control the extraction The temperature is 65°C, extract for 3 hours, then raise the temperature to 85°C to inactivate cellulase, collect the extract, cool to room temperature, centrifuge at 4000 rpm, collect the supernatant, and obtain substance A;
[0019] 2) Add 1.2g of lipase to substance A, adjust the pH=6.0, control the extraction temperature and extract at 30°C for 3.5h, then raise the temperature to 68°C to inactivate the lipase, collect the extract, cool to room temperature, and pass through the oil-water separator Separate and collect the water layer to obtain substance B;
[0020] 3) Substance B was concentrated under reduced pressure at 0.07Mpa to 1 / 6 of the original volume, and ethanol was added to the concentrated solution so that the content...
Embodiment 2
[0022] 1) Weigh 150g of dried fenugreek seeds, crush them in a pulverizer, pass through a 200-mesh sieve, and dissolve them fully with 10500mL of distilled water; add 0.6g of cellulase under stirring, adjust the pH to 3.0, and control the extraction temperature to 50 ℃, extract for 2 hours, then raise the temperature to 80 ℃ to inactivate cellulase, collect the extract, cool to room temperature, centrifuge at 4200 rpm, collect the supernatant, and obtain substance A;
[0023] 2) Add 1.05g of lipase to substance A, adjust the pH=5.0, control the extraction temperature to 60°C, and extract for 4 hours; then raise the temperature to 70°C to inactivate the lipase, collect the extract, cool to room temperature, and pass through the oil-water separator Separate and collect the water layer to obtain substance B;
[0024] 3) Substance B is concentrated under reduced pressure at 0.08Mpa to 1 / 5 of the original volume, and ethanol is added to the concentrated solution so that the ethanol...
Embodiment 3
[0026] 1) Weigh 100g of dried fenugreek seeds, crush them in a pulverizer, pass through a 200-mesh sieve, dissolve them fully with 10,000mL of distilled water, add 0.5g of cellulase under stirring, adjust the pH to 6, and control the extraction temperature to 30 ℃, extract for 5 hours; then raise the temperature to 82 ℃ to inactivate cellulase; collect the extract, cool to room temperature, centrifuge at 5000 rpm, collect the supernatant, and obtain substance A;
[0027] 2) Add 1.0 g of lipase to substance A, adjust the pH to 8.0, control the extraction temperature to 50°C, and extract for 3 hours; then raise the temperature to 65°C to inactivate the lipase, collect the extract, cool to room temperature, and separate through an oil-water separator , collect the water layer to obtain substance B;
[0028] 3) Substance B was concentrated under reduced pressure at 0.09Mpa to 1 / 7 of the original volume, and ethanol was added to the concentrated solution so that the ethanol content...
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