Method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling

A technology of fenugreek gum and coupling method, which is applied in the field of double-enzyme coupling extraction of fenugreek gum, can solve the problems of changes in biological activity of natural products, time-consuming and labor-intensive extraction with hot water, and low extraction rate, so as to shorten the time , mild reaction conditions and simple process

Inactive Publication Date: 2012-12-12
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main methods for preparing fenugreek gum include hot water extraction, ultrasonic-assisted extraction, and microwave-assisted extraction. Conventional hot water extraction is time-consuming and labor-intensive, and the extraction rate is not high; ultrasonic-assisted extraction and microwave-assisted extraction often make the extract The structure of the natural product is changed, so that the biological activity of the natural product is changed or lost

Method used

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  • Method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling
  • Method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1) Weigh 200g of dried fenugreek seeds, crush them in a pulverizer, pass through a 200-mesh sieve, add them into 10000mL of distilled water, stir to dissolve them fully; add 0.4g of cellulase, adjust the pH to 4.5, and control the extraction The temperature is 65°C, extract for 3 hours, then raise the temperature to 85°C to inactivate cellulase, collect the extract, cool to room temperature, centrifuge at 4000 rpm, collect the supernatant, and obtain substance A;

[0019] 2) Add 1.2g of lipase to substance A, adjust the pH=6.0, control the extraction temperature and extract at 30°C for 3.5h, then raise the temperature to 68°C to inactivate the lipase, collect the extract, cool to room temperature, and pass through the oil-water separator Separate and collect the water layer to obtain substance B;

[0020] 3) Substance B was concentrated under reduced pressure at 0.07Mpa to 1 / 6 of the original volume, and ethanol was added to the concentrated solution so that the content...

Embodiment 2

[0022] 1) Weigh 150g of dried fenugreek seeds, crush them in a pulverizer, pass through a 200-mesh sieve, and dissolve them fully with 10500mL of distilled water; add 0.6g of cellulase under stirring, adjust the pH to 3.0, and control the extraction temperature to 50 ℃, extract for 2 hours, then raise the temperature to 80 ℃ to inactivate cellulase, collect the extract, cool to room temperature, centrifuge at 4200 rpm, collect the supernatant, and obtain substance A;

[0023] 2) Add 1.05g of lipase to substance A, adjust the pH=5.0, control the extraction temperature to 60°C, and extract for 4 hours; then raise the temperature to 70°C to inactivate the lipase, collect the extract, cool to room temperature, and pass through the oil-water separator Separate and collect the water layer to obtain substance B;

[0024] 3) Substance B is concentrated under reduced pressure at 0.08Mpa to 1 / 5 of the original volume, and ethanol is added to the concentrated solution so that the ethanol...

Embodiment 3

[0026] 1) Weigh 100g of dried fenugreek seeds, crush them in a pulverizer, pass through a 200-mesh sieve, dissolve them fully with 10,000mL of distilled water, add 0.5g of cellulase under stirring, adjust the pH to 6, and control the extraction temperature to 30 ℃, extract for 5 hours; then raise the temperature to 82 ℃ to inactivate cellulase; collect the extract, cool to room temperature, centrifuge at 5000 rpm, collect the supernatant, and obtain substance A;

[0027] 2) Add 1.0 g of lipase to substance A, adjust the pH to 8.0, control the extraction temperature to 50°C, and extract for 3 hours; then raise the temperature to 65°C to inactivate the lipase, collect the extract, cool to room temperature, and separate through an oil-water separator , collect the water layer to obtain substance B;

[0028] 3) Substance B was concentrated under reduced pressure at 0.09Mpa to 1 / 7 of the original volume, and ethanol was added to the concentrated solution so that the ethanol content...

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Abstract

The invention provides a method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling. The method includes that common fenugreek seeds are used as raw materials, the common fenugreek seeds are subjected to wall breaking by means of cellulose after crushed and subjected to solid-liquid separation after enzyme activities are passivated, lipase is added into a clear liquid for demulsification, oil water separation is performed after enzyme activities are passivated, and an aqueous layer is concentrated and dried to obtain the bottle gourd Pakistan rubber. According to determination, the yield of the bottle gourd Pakistan rubber is 8%-10%. The method for extracting the bottle gourd Pakistan rubber by dual-enzyme coupling is simple in process, mild in reaction condition, short in process period, low in energy consumption and high in efficiency; and organic solvents are not used so that the method is safe and environment-friendly.

Description

technical field [0001] The invention relates to a method for extracting fenugreek gum, in particular to a method for extracting fenugreek gum through double-enzyme coupling. Background technique [0002] Fenugreek (Trigonella foenum-graecum L.) is an annual herb belonging to the genus Fenugreek of the Fabaceae Papilionaceae, widely distributed in Asia, Africa and Europe. The aerial part of the fenugreek plant has a special aroma after drying, which can prevent insects and lice. Its seeds are Chinese herbal medicines and are also used as raw materials for the pharmaceutical industry. In the food industry, it is widely used in coffee, essence, etc., as well as in tobacco and candy. Fenugreek gum is a polysaccharide gum extracted from the endosperm of fenugreek seeds, and its main component is galactomannan. Fenugreek gum is a natural polymer with good water solubility and crosslinking, strong thickening ability and suspension ability. It can be used as a fracturing fluid th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 张继赵保堂姚健梁俊玉李晶晶陶彦佼
Owner NORTHWEST NORMAL UNIVERSITY
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