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Intelligent nano delivery system, preparation method and application thereof

A nanoparticle and release system technology, which is applied in the fields of respiratory system diseases, nervous system diseases, cardiovascular system diseases, etc.

Inactive Publication Date: 2013-01-09
北京圣琳贝嘉医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are several generic statins on the market, Lipitor is more cost-effective for patients with severe dyslipidemia and no other high-risk factors. Wide range of effective doses, there is no such rapid and effective control of blood lipids as shown in the ASCOT trial

Method used

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  • Intelligent nano delivery system, preparation method and application thereof
  • Intelligent nano delivery system, preparation method and application thereof
  • Intelligent nano delivery system, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Nanoparticle preparation and determination of characterization parameters.

[0070] Polylactic acid-glycolic acid copolymer (PLGA) (90 mg; 50:50, viscosity 1.3) was dissolved in 3 ml of chloroform, and dimethyl tartrate or diester of tartrate (density 1.2 g / ml) was dissolved in the above polymer solution. Bovine serum albumin (BSA) (30 mg) was dissolved in 300 microliters of water, vortexed and mixed for 1 minute, the protein solution was emulsified in the above PLGA solution, and then at a power output of 55 watts, the probe was used for ultrasonic dispersion 2 minute. At the same energy output setting, vortex mixing and ultrasonic dispersion for 2 minutes, the emulsion is further emulsified into 12 ml of 2% polyvinyl alcohol (PVA) solution (the average molecular weight of PVA: 30,000-70,000). The PVA solution was filtered through a 0.22 micron syringe filter and saturated with chloroform before use. Add a few drops of chloroform to the PVA solution, shake well, and t...

Embodiment 2

[0073] Preparation of human telomerase nanoparticles, cell delivery and determination of chromosomal telomere DNA length extension activity.

[0074] The PLGA nanoparticles containing human recombinant telomerase or hTert protein are prepared by using a double-emulsion solvent evaporation technique, and the preparation process is the same as in Example 1. In this embodiment, telomerase holoenzyme and telomerase hTert catalytic protein subunits are respectively encapsulated in a biodegradable PLGA polymer to prepare nanoparticles. The telomerase component of transfected human fibroblasts is slowly released, which therapeutically extends the length of cell chromosome telomeres.

[0075] The fibroblasts (ATCC, SCRC-1041) to be transfected were seeded in 4 6-well plates (cell density 1.4 × 10 5 Cells / wells), the air contains complete growth medium (containing 15% V / V serum), each nanoparticle sample is placed in 3 wells, and cells of the same density are seeded in the wells witho...

Embodiment 3

[0080] Preparation and sustained release characteristics of SOD nanoparticles.

[0081] The preparation of superoxide dismutase (SOD) nanoparticles containing dimethyltartaric acid (DMT) is the same as the preparation of BSA protein nanoparticles (see Example 1). In general, 10% of DMT is used in nanoparticle preparations; the loading of SOD in nanoparticles is calculated by measuring the content of SOD that is not encapsulated by nanoparticles. For this reason, the cleaning solution generated during the preparation of nanoparticles should be collected and analyzed separately, and the SOD enzyme activity in the sample should be measured and calculated separately using a standard SOD enzyme activity detection kit. The load of superoxide dismutase nanoparticles is 4.5%, and the encapsulation rate is 75% (that is, 75% of the supplementary protein is encapsulated in the nanoparticles).

[0082] In order to prove the sustained-release properties of nanoparticles, the inventors of th...

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Abstract

The invention discloses an intelligent nanoparticle drug release system capable of delivering biomacromolecules and small organic molecules to cells, a preparation method of the intelligent nanoparticle drug release system, and application of the intelligent nanoparticle drug delivery system in cell delivering of biomacromolecules and small organic molecules (including drugs). By delivering biochemical substances of drug molecules, cell nutrients and cell expellant, physiological metabolism and pathological metabolism are intervened. Molecule delivery of an embodiment of the intelligent nanoparticle drug delivery system includes but is not limited to the following molecules: telomerase cell delivery; antioxidant enzyme (such as SOD (superoxide dismutase) and HPX (hydrogen peroxidase)) cell delivery; cancer suppressor protein (such as p53 and PTEN(phosphatase and tensin homolog deleted on chromosome ten)) cell delivery; and organic chemical and biochemical small molecules (such as atorvastatin-lipitor and coenzyme Q-10) cell delivery. The molecule slow release and control of the nanoparticle drug delivery system has uniqueness and targeting so that the nanoparticle drug delivery system is intelligent.

Description

technical field [0001] The invention relates to an intelligent nano-delivery system for cell delivery of biomacromolecules and small organic molecules (including drugs), its preparation method and application in drug cell delivery. The present invention further provides a nanotechnology platform for cell delivery. Background technique [0002] Human telomerase reverse transcriptase (hTert) is a catalytic subunit or catalytic molecular entity. This catalytic subunit has been shown to simultaneously assemble into the telomerase holoenzyme, a complex of the holoenzyme and another RNA component (hTert) assembled in human cells. The protein component of human telomerase reverse transcriptase (hTert) is a catalytic molecular subunit. Experiments show that this subunit can simultaneously form a holoenzyme with the RNA component (hTert). In nature, telomerase repairs chromosomal DNA by lengthening the telomeres at the ends of chromosomes. Telomeres of abnormal length or accelera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/34A61K47/32A61K47/42A61K9/14A61K31/122A61K31/40A61K38/02A61K38/10A61K38/17A61K38/44A61K38/45A61P3/06A61P3/10A61P9/10A61P11/06A61P17/00A61P19/02A61P25/16A61P25/28A61P29/00A61P35/00A61P37/02A61P37/08A61P39/06
Inventor 翟国堂
Owner 北京圣琳贝嘉医药科技有限公司
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