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Comprehensive method for preparation of raffinose and gossypol from cottonseed meal

A comprehensive method, raffinose technology, applied in the preparation methods of peptides, chemical instruments and methods, preparation of sugar derivatives, etc., can solve the problems of incapable of maximizing the use of cottonseed meal value, waste of resources, etc., and achieve maximum value. , the effect of maximizing value

Active Publication Date: 2013-01-30
邯郸晨光植物蛋白有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, raffinose and gossypol are extracted separately, and gossypol will be discharged as waste when preparing raffinose, which will waste resources and cannot maximize the value of cottonseed meal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: The comprehensive method for preparing raffinose and gossypol from cottonseed meal adopts the following process steps.

[0024] (1) Cotton embryo first passed 6 # The cottonseed oil is extracted by solvent oil spray; the cottonseed meal after leaching the oil is transported to the extractor by the scraper, and the raffinose and gossypol are sprayed with 90% methanol. The extraction temperature is 55°C and the extraction time is 60min. Obtain an extract containing raffinose and gossypol;

[0025] (2) The extract is filtered with a 400-mesh filter bag, and methanol is recovered under the conditions of a vacuum of 0.095Mpa and 60°C to obtain a raffinose solution containing a solid phase. The solution is separated and separated to obtain a raffinose-containing aqueous phase and cotton-containing The solid phase of phenol;

[0026] (3) Filter the aqueous phase containing raffinose with a 500 mesh filter bag, add 5% (g / ml) calcium hydroxide to the filtered aqueous phase,...

Embodiment 2

[0030] Example 2: The comprehensive method for preparing raffinose and gossypol from the cottonseed meal adopts the following process steps.

[0031] (1) Cotton embryo first passed 6 # The cottonseed oil is extracted by solvent oil spraying; the cottonseed meal after leaching the oil is transported by the scraper to the extractor, and the raffinose and gossypol are sprayed with 95% ethanol. The extraction temperature is 52℃ and the extraction time is 90min. Obtain an extract containing raffinose and gossypol;

[0032] (2) The extract was filtered with a 300-mesh filter bag, and ethanol was recovered under the conditions of a vacuum of 0.095Mpa and 55°C to obtain a raffinose solution containing a solid phase. After standing for 120 minutes, the aqueous phase and the aqueous phase containing raffinose were separated. The solid phase of gossypol;

[0033] (3) Filter the aqueous phase containing raffinose with a 600 mesh filter bag, add 2% (g / ml) iron sulfate to the filtered aqueous pha...

Embodiment 3

[0037] Example 3: The comprehensive method for preparing raffinose and gossypol from the cottonseed meal adopts the following process steps.

[0038] (1) Cotton embryo first passed 6 # The cottonseed oil is extracted by solvent oil spraying; the cottonseed meal after leaching the oil is transported by the scraper to the extractor, and the raffinose and gossypol are sprayed with ethanol. The extraction temperature is 45℃ and the extraction time is 120min. Gossypol extract;

[0039] (2) The extract was filtered with a 600-mesh filter bag, and ethanol was recovered under the conditions of a vacuum of 0.09Mpa and 65°C to obtain a raffinose solution containing a solid phase. After standing for 120 minutes, the aqueous phase and the aqueous phase containing raffinose were separated. The solid phase of gossypol;

[0040] (3) The aqueous phase containing raffinose is filtered with a 400 mesh filter bag, and the filtered aqueous phase is added with 1% (g / ml) calcium oxide and ferric chloride...

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PUM

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Abstract

The invention discloses a comprehensive method for preparation of raffinose and gossypol from cottonseed meal. The method comprises the technological steps of: (1) extracting oil-extracted cottonseed meal with a polar solvent so as to obtain an extracted solution; (2) filtering the extracted solution, and removing an organic solvent in vacuum so as to obtain an aqueous phase and a solid phase; (3) subjecting the aqueous phase to filtration, flocculation, decoloration and desalination, decoloration, as well as desolventizing, thus obtaining raffinose; (4) first adding enzymes into the solid phase to carry out hydrolysis, then using an acid to adjust the solution to acidic, and performing precipitation and separation, thus obtaining a solid; (5) adding ether, halohydrocarbon, ester and / or low level ketone solvents to dissolve gossypol, and then filtering out impurities to obtain a gossypol-containing filtrate; and (6) subjecting the gossypol-containing filtrate to crystallization and crystal growing under nitrogen protection, then conducting filtration and drying, thus obtaining acicular crystals. The method can realize combined industrial production of dephenolized cottonseed protein, raffinose and gossypol, is suitable for large-scale production, and realizes comprehensive processing as well as maximum valuation of cottonseeds.

Description

Technical field [0001] The invention relates to a comprehensive utilization method of cottonseed meal, in particular to a comprehensive method for preparing raffinose and gossypol from cottonseed meal. Background technique [0002] my country is a large cotton growing country. Cotton is the only genetically modified crop grown in large areas in my country. Cotton is grown in many provinces and regions in my country, mainly in Xinjiang, Hebei, Shandong, Anhui, Hubei, Henan and other places. Cottonseed resources are very rich. The inherent active ingredient of cottonseed is gossypol, which is mainly found in cotton kernels. The cottonseed meal after oil extraction contains about 4.2% raffinose and about 0.1-1% gossypol. The defatted cotton meal is a rich source of raffinose. Raffinose is a trisaccharide, which is a combination of galactose, glucose and fructose. It is an oligosaccharide and is a non-reducing sugar with good physiological activity. It is easily soluble in water but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/08C07C47/57C07C45/78C07K14/415C07K1/14
Inventor 陈运霞张燕飞宋伟光周冬丽琚瑶
Owner 邯郸晨光植物蛋白有限公司
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