Method for preparing marine environment-friendly calcium citrate hard tissue repair material

Abstract

The invention discloses a method for preparing a marine environment-friendly calcium citrate hard tissue repair material. The method comprises the following steps of: (1) cleaning fresh oyster shells in a non-pollution sea area physicochemically, soaking the cleaned fresh oyster shells in weak acid, and washing by flowing water until impurities are removed completely; (2) crushing the clean oyster shells into granules by a ball grinding mill, and sieving with a 200-mesh sieve; (3) reacting oyster shell powder with a supersaturated solution of citric acid, centrifuging, taking precipitates out, and drying by a forced draft drying machine to obtain calcium citrate powder; and (4) filtering the prepared calcium citrate powder by using a 100-mesh filter sieve, filtering by using a 60-mesh filter sieve, and taking filtered substances out to obtain calcium citrate granules. The marine environment-friendly calcium citrate hard tissue repair material has high mechanical strength and appropriate granular sizes, and the degradation time of the material is prolonged by tight bonding force among crystals, so that the material can be degraded slowly in bodies, and calcium ions are released stably to create a local high-calcium environment; and the marine environment-friendly calcium citrate hard tissue repair material has an obvious therapeutic effect on bone loss, osteoporosis and bone tumors, and has a wide application value in biological tissue repair.

Description

technical field [0001] The invention relates to a novel marine environment-friendly calcium citrate hard tissue repair material. Background technique [0002] Various types of bone defects are very common in clinical practice. Bone nonunion caused by trauma, treatment of bone defects, limb salvage treatment of bone tumors, and some reconstructive operations often involve bone grafting. Currently, bone graft materials in clinical use include autologous bone, allogeneic bone, and various synthetic graft material substitutes. All these bone graft materials have at least one of the following biological properties: (1) Osteoconductivity: provide a scaffold for the ingrowth of blood vessels and the formation of new bone; (2) Osteoinduction : Contains osteoinductive protein, which can stimulate the mesenchymal stem cells around the bone grafting area to differentiate into chondrocytes or osteoblasts to form new bone; (3) Osteogenesis: Contains osteogenic cells, osteoblasts Osteoc...

AI Technical Summary

Problems solved by technology

Biomaterials based on hydroxyapatite and tricalcium phosphate are still difficult to completely biodegrade or absorb in the bone defect after a long period of time, which will eventually affect the formation of new bone tissue; bone filling materials based on calcium sulfate hemihydrate, due to The price is expensive, its degradation rate is too fast, and the calcium concentration around the material is 14mmol / L, which ultimately affects the proliferation and mineralization of surrounding bone cells; although bioglass has a certain bone activation effect, it has the disadvantages of high brittleness and low bending strength

The application of these materials is limited due to their own defects

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