Shinella sp. and application thereof to micro-biologically degrading acetaminophen

A technology for acetaminophen and microbial degradation, applied in microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve problems such as environmental pollution, major safety risks, DNA strand breaks, etc., and achieve a simple and good preparation process. Application prospect, rapid degradation effect

Active Publication Date: 2013-03-13
沛县度创科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When this type of drug is used after drinking alcohol, it will greatly affect the metabolism of sugar, fat, energy and vitamins, and aggravate poisoning. Excessive use of acetaminophen will cause methemoglobinemia, DNA chain breaks, and inhibit DNA replication and Repair the system, but also cause the proliferation of breast cancer cells
A large part of acetaminophen does not undergo metabolism in the body, but is directly excreted from the body, polluting the environment
At the same time, sewage treatment plants cannot completely remove paracetamol, and the removal rate of general sewage treatment plants is only about 85%
Acetaminophen and its degradation intermediates exposed in the environment will cause harm to humans and animals, and acetaminophen has a greater safety risk to target organisms and non-target organisms
The harm and risk of acetaminophen contamination will be aggravated by environmental actions such as bioaccumulation

Method used

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  • Shinella sp. and application thereof to micro-biologically degrading acetaminophen
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  • Shinella sp. and application thereof to micro-biologically degrading acetaminophen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Screening and identification of strains

[0043] 1) culture medium

[0044] Preparation of inorganic salt medium: NaCl 1g, K 2 HPO 4 1.5g, KH 2 PO 4 0.5g, (NH 4 ) 2 SO 4 1.5g, MgSO 4 0.1g, 1ml trace element solution, made up to 1000ml with deionized water, mixed and stirred evenly, natural pH value, made after high-pressure steam sterilization (121°C, 20min), and each liter of trace element solution contains MnSO 4 ﹒ h 2 O 0.13 g , ZnCl 2 0.23g, CuSO 4 ﹒ h 2 O 0.03g, CoCl 2 ﹒ 6H 2 O 0.42g, Na 2 MoO 4 ﹒ 2H 2 O 0.15g, AlCl 3 ﹒ 6H 2 O 0.05g, make up to 1000ml with deionized water.

[0045] Enriched culture solution: add acetaminophen to the inorganic salt medium so that the final concentration of acetaminophen is 200 mg / L.

[0046] Preparation of LB liquid medium: Yeast powder 10g, peptone 5.0g, sodium chloride 10.0g, deionized water to make up to 1000ml, mixed and stirred evenly, natural pH value, prepared after high-pressure s...

Embodiment 2

[0053] Embodiment 2: the preparation that contains bacterial cell suspension

[0054] (1) Slant culture: Inoculate Shennella HZA2 on the slant medium and culture at 30°C for 3-5 days to obtain the slant; the final concentration of the slant medium is composed of: 10g of yeast powder, 5.0g of peptone, chlorine Sodium chloride 10.0g, agar 20g, deionized water 1000ml;

[0055] (2) Seed culture: pick an inoculation loop from the slant of the bacteria in step (1) and inoculate it into the inorganic salt medium, culture at 30°C for 3-5 days, and obtain the seed liquid; the final concentration of the inorganic salt medium is Composition is with embodiment 1;

[0056] (3) Expansion culture: Inoculate the seed solution obtained in step (2) into LB liquid medium (100 mL) at an inoculum volume concentration of 10-20%, and cultivate it with shaking at 30°C and 150 rpm until the logarithmic growth phase, and obtain Bacterial solution, centrifuge the bacterial solution (6000rpm, 5min), di...

Embodiment 3

[0057] Embodiment 3: Degradation experiment of acetaminophen

[0058] 1) Detection of bacterial concentration and acetaminophen content in inorganic salt medium:

[0059] The growth of bacteria is detected by ultraviolet spectrophotometer, which is expressed by measuring the absorbance value of bacteria in the culture solution at 600nm.

[0060] In this experiment, reversed-phase high-performance liquid chromatography was used to detect the residual amount of acetaminophen in the inorganic salt medium. Reversed-phase high-performance liquid chromatography detection conditions: mobile phase is methanol: H 2 O=15:85 (volume ratio), the analytical column is a Grace Alltima C18 column (4.6×250mm, 5μm), the flow rate is 0.8ml / min, the injection volume is 20μl, and the column temperature is 30°C.

[0061] 2) Paracetamol degradation experiment:

[0062] Take four 250ml Erlenmeyer flasks, add 100ml of inorganic salt culture medium, and add acetaminophen after high-pressure steam st...

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Abstract

The invention provides a novel efficient acetaminophen degrading bacterium-Shinella sp. HZA2 and an application thereof to micro-biologically degrading acetaminophen. The strain was collected in the China Center for Type Culture Collection (CCTCC), with the collection date being October 15, 2012 and collection number being CCTCC No: M2012405. The address of the CCTCC is Wuhan University, Wuhan, China and the postcode is 430072. The Shinella sp. HZA2 can be applied to degrading acetaminophen in water and soil by way of direct addition and can safely, efficiently and quickly degrade residual acetaminophen on such objects as water and soil. The fungicides containing the strain are simple in preparation processes, low in costs and convenient to use and have good application prospects.

Description

(1) Technical field [0001] The invention relates to a novel high-efficiency acetaminophen-degrading bacterium—Shinella sp. HZA2, and its application in microbial degradation of acetaminophen. (2) Background technology [0002] Acetaminophen (Acetaminophen), the drug name is paracetamol (Paracetamol, APAP, acetaminophen). It is a white crystalline powder with antipyretic and analgesic effects. It is often used as an anti-inflammatory drug and analgesic. It has been widely used since the 1950s. Its molecular structure is shown in formula (I). Acetaminophen is odorless, bitter, melting point 169-171°C, relative density 1.293 (21 / 4°C), soluble in alcohol, acetone and hot water, insoluble in petroleum ether and benzene, pH value of saturated aqueous solution is 5.5- 6.5. [0003] [0004] Acetaminophen, as a routine analgesic and fever-reducing prescription drug, is widely used all over the world. Long-term use of acetaminophen, the toxic intermediates produced by its met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/01C02F101/38A62D101/28
Inventor 马云刘学虎
Owner 沛县度创科技发展有限公司
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