Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of flow injection chemiluminiscence immuno sensor for detecting tumor marker

A tumor marker and flow injection technology, applied in the field of immune marker detection, can solve the problems of incompetence, time-consuming, expensive instruments, etc., to avoid the influence of subjective factors, the determination operation is fast and simple, and the biocompatibility is good. Effect

Inactive Publication Date: 2013-03-13
UNIV OF JINAN
View PDF7 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these immunoassay methods generally take a long time and have cumbersome operations such as adding samples, incubating, and washing, and the operators have more contact with immune substances, which may cause harm to the health of the operators.
Moreover, some detection methods with fast determination speed, high sensitivity and low detection performance require expensive instruments and cannot be popularized in developing countries.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of flow injection chemiluminiscence immuno sensor for detecting tumor marker
  • Preparation method and application of flow injection chemiluminiscence immuno sensor for detecting tumor marker
  • Preparation method and application of flow injection chemiluminiscence immuno sensor for detecting tumor marker

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] A method for preparing a flow injection chemiluminescent sensor for detecting immune markers, comprising the following steps:

[0035] (1) The flow injection chemiluminescence flow cell was prepared by using PMMA;

[0036] (2) Prepare carbon quantum dots, carbon-coated ferric oxide, silicon dioxide and empty-shell gold nanoparticles according to existing methods;

[0037] (3) Decorate the luminescent carbon quantum dots on the carbon-coated ferric oxide, and then decorate the surface with the primary antibody;

[0038] (4) Decorate the empty-shell gold nanoparticles on the silica particles, and then modify the secondary antibody and glucose oxidase on the empty-shell gold particles;

[0039] (5) Combine the prepared chemiluminescence sensor with a flow injection chemiluminescence instrument to detect immune markers.

[0040] The preparation of the luminescent carbon quantum dot modified carbon-coated ferric oxide of the present invention comprises the following steps:...

Embodiment 1

[0053] Example 1 (Embryonic antigens such as AFP))

[0054] (1) Select AFP with higher clinical incidence for determination;

[0055] (2) Preparation of carbon-coated ferric oxide: immersed ferric tetroxide in nitric acid solution and ultrasonicated for 10 min, then separated by the action of a magnet, rinsed twice with water, and then sonicated in 5% glucose solution for 10 min. min, rinsed twice with water, and finally the aqueous solution of ferric oxide was reacted in an autoclave at 180 °C for 10 h;

[0056] (3) Preparation of carbon quantum dots: in a 7.0 phosphate buffer solution, the graphite electrode was used as the working electrode, the silver / silver chloride electrode was used as the reference electrode, and the platinum electrode was used as the counter electrode. 0.1 V / s sweep rate reaction for 24 h, centrifugation, and secondary water washing;

[0057] (4) The carbon-coated ferric oxide prepared in (2) was immersed in 0.2% PDDA and stirred for 20 minutes to...

Embodiment 2

[0073] Example 2 (Carbohydrate markers such as CA19-9)

[0074] (1) Select CA19-9 with higher clinical incidence for determination;

[0075] (2) Preparation of carbon-coated ferric oxide: immersed ferric tetroxide in nitric acid solution and ultrasonicated for 10 min, then separated by the action of a magnet, rinsed twice with water, and then sonicated in 5% glucose solution for 10 min. min, rinsed twice with water, and finally the aqueous solution of ferric oxide was reacted in an autoclave at 180 °C for 10 h;

[0076] (3) Preparation of carbon quantum dots: In PBS with pH 7.0, the graphite electrode was used as the working electrode, the silver / silver chloride electrode was used as the reference electrode, and the platinum electrode was used as the counter electrode. V / s scan rate reaction for 24 h, centrifugation, and secondary water washing;

[0077] (4) The carbon-coated ferric oxide prepared in (2) was immersed in 0.2% PDDA and stirred for 20 minutes to make the surf...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a flow injection chemiluminiscence detection sensor for a tumor marker. A preparation method of the flow injection chemiluminiscence detection sensor comprises the following steps of: preparing a flow injection chemiluminescence flow-through cell by utilizing polymethyl methacrylate; preparing carbon qunatum points, ferroferric oxide coated with carbon, silicon dioxide and hollow gold nano particles according to the existing method; coating the carbon qunatum points on the surfaces of magnetic particles; modifying primary antibodies on the magnetic particles coated with the carbon qunatum points; modifying the hollow gold nano particles on the silicon dioxide; coating secondary antibodies on the hollow gold nano particles positioned on the surface of the silicon dioxide; and carrying out chemiluminescence detection by utilizing flow injection. The flow injection chemiluminiscence detection sensor disclosed by the invention has the advantages of high specificity, high sensitivity, easiness for operation and low detection line.

Description

Technical field [0001] The present invention involves the field of immunohistan detection technology. More specifically, the preparation of a mobile injection chemical emitting immune sensor also involves the method of using the mobilization chemical emitting sensor to measure the immune bid. Background technique [0002] In recent years, immune analysis has been a clinical examination of a diagnosis and treatment of an important tumor disease.For a long time, malignant tumors, that is, cancer is a serious threat to human health and life safety.Cancer cells refer to a new creature produced by vicious transformation and cloning hyperplasia under the action of carcinogenic factors under the action of carcinogenic factors.In addition to their own growth and out of control, cancer cells can also invade the normal tissue around the surroundings and can even transfer to other parts of the body through the circulatory system or lymphatic system in the body, causing physical lesions or e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/574G01N21/76G01N33/531
Inventor 颜梅楚成超葛慎光葛磊张彦王盼盼李伟平李龙李蒙刘芳刘伟艳王衍虎于京华
Owner UNIV OF JINAN
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com