Longan functional drink and method for preparing same
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A technology for functional beverages and longan, applied in the food field, can solve the problems of insufficient utilization of longan nutrients and no improvement of nutrients.
Inactive Publication Date: 2013-04-03
JIMEI UNIV
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The development of products such as longan juice, longan paste, longan tea, longan soup, longan jelly, and longan granules simply dissolves some ingredients in longan, and some allergic ingredients in longan can easily cause allergic reactions. Constipation, short red urine, mouth and eye sores, toothache, acne and other symptoms; longan wine, longan brandy and other products mainly use yeast to ferment the carbohydrates in longan to produce etha
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Embodiment 1
[0033] Example 1: Cloning of the manganese superoxide dismutase gene and expression in Lactobacillus bulgaricus
[0034] (1) Preparation of Bulgarian lactic acid bacteria containing manganese superoxide dismutase gene
[0035] Amplification primers were designed according to the nucleotide sequence of Lactococcus superoxide dismutase gene (SOD) in GenBank (Genbank sequence number: NC_002662):
[0036] P1: 5'-CGCGGATCCGCGATGGCATTTACATTA-3'; (SEQ ID NO: 1)
[0037] P2: 5'-CCGCTCGAG CGGTTATTTTGCTTTAGCAT-3'. (SEQ ID NO:2)
[0038] PCR amplification program (95°C, 5min; 95°C, 1min; 55°C, 1min; 72°C, 1min; 30 cycles; 72°C, 7min), the template was derived from Lactococcus lactis (purchased from Guangdong Institute of Microbiology species collection) genomic DNA. The carrier plasmid is food-grade PMG36e (purchased from Shanghai Beinuo Biotechnology Co., Ltd.);
[0039] (2) Lactobacillus delbrueckii subsp. bulgaricus was transformed by electroporation with the recombinant plasmid ...
Embodiment 2
[0041] Mix 5 kg of longan meat, 0.3 kg of red dates, and 0.1 kg of wolfberry, add water to 50 L, boil to 90 ° C and keep warm for 30 minutes, grind and filter with a homogenizer to obtain longan red dates and wolfberry juice, and inoculate 2% of the manganese superoxide obtained in Example 1 Lactobacillus bulgaricus with the dismutase gene is fermented in a closed anaerobic manner at 40°C for 3 days, and after the fermentation is completed, it is rapidly cooled to below 10°C for storage.
Embodiment 3
[0043] Mix 5 kg of longan meat, 0.4 kg of red dates, and 0.02 kg of rosemary, add water to 50 L, boil to 80°C and keep warm for 40 minutes, grind and filter with a homogenizer to obtain longan and red date juice, and inoculate 3% of the manganese superoxide obtained in Example 1. The Lactobacillus bulgaricus with the dismutase gene was fermented in a closed anaerobic manner at 37°C for 4 days. After the fermentation, it was quickly cooled to below 10°C for storage.
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Abstract
The invention relates to a longan functional drink and a method for preparing the same. The method comprises the following steps of: mixing desired longan pulp with other various raw materials and adding water to the mixture to a certain volume, boiling the mixture until the temperature is in the range of 80-100 DEG C, preserving heat for 20-40 minutes, grinding by using a pulp refiner until the particle granularity is about 15-20 microns, and filtering to obtain longan mixed juice; inoculating the longan mixed juice with sbacillus containing a manganese superoxide dismutase gene for fermentation culture; and keeping in cold storage, thereby obtaining the longan functional drink. The obtained longan functional drink is advantageous for improving the digestion absorption efficiency of the human body to food, improving the feeding of satiety after eating, reducing the possibility of abdominal distension exhaust, promoting normal defecation, reducing the pressure of the gastrointestinal tract, promoting vigor, promoting the health of the circulatory system, helping the body to adjust operation of the immunologic function and enhancing the human body defense mechanism; besides, the drink is advantageous for clearing away superoxide radicals generated in the human body and protecting the cells against damage, and has the characteristics of resisting aging and radiation, improving the tolerance of the cells to oxygen emergency reaction and the like.
Description
technical field [0001] The invention relates to the field of food, in particular to a longan functional drink. Background technique [0002] Longan is a unique tropical fruit in southern my country. It is mainly produced in Fujian, Guangdong and other places. The flesh is white and transparent. Minerals, with high nutritional value, known as "Southern Ginseng". Traditional Chinese medicine studies have shown that longan has the effects of nourishing yin and kidney, nourishing the middle and Qi, nourishing the lungs, appetizing and benefiting the spleen, and has the effects of treating post-illness weakness, anemia, chlorosis, neurasthenia, and postpartum anemia. [0003] In recent years, the production scale and output of longan have been greatly increased, but because longan is not easy to store, it is easy to cause longan to be lost due to corruption, which seriously restricts the development of longan industry. The efficiency and the enthusiasm of fruit growers are of gr...
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