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Preparation and application of biological control bacterial strain for diseases of ginseng plant

A technology for plant disease control and ginseng, applied in microorganism-based methods, chemicals for biological control, applications, etc., can solve the problems of environmental pollution pathogens, excessive pesticide residues, and quality degradation, and achieves convenient operation and low cost. , prepare a simple effect

Inactive Publication Date: 2013-04-03
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of disease prevention and control is to use chemical pesticides to control the disease during the disease occurrence period, but long-term use of chemical pesticides or irrational use will lead to excessive pesticide residues in ginseng, quality decline, environmental pollution and pathogenic bacteria. The emergence of drug resistance and other adverse consequences

Method used

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  • Preparation and application of biological control bacterial strain for diseases of ginseng plant
  • Preparation and application of biological control bacterial strain for diseases of ginseng plant
  • Preparation and application of biological control bacterial strain for diseases of ginseng plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Screening of ginseng endogenous antagonistic bacterial strain NJ13

[0023] From June to September 2010, fresh ginseng ( Panax ginseng ) samples, the collection part is the whole plant, and the growth period is 4 years. A total of 152 strains of endophytic bacteria were isolated from different organs of ginseng roots, stems and leaves. The screening of ginseng antagonistic endophytes is divided into primary screening and secondary screening. Among them, the NJ13 strain isolated from the ginseng plant stem; on October 18, 2012, it was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee, which is referred to as CGMCC, and the registration number of the preservation center is CGMCC No.6679. Address: Institute of Microbiology, Chinese Academy of Sciences, Beichen West Road, Chaoyang District, Beijing, Zip Code: 100101; Classification Name: Methylotrophic Bacillus ( Bacillus methylotrophicus ).

Embodiment 2

[0024] Example 2 Determination of antibacterial activity of ginseng endogenous antagonistic bacterial strain NJ13

[0025] The antibacterial activity of the isolated ginseng endophytic antagonistic bacteria NJ13 was tested by confrontation method. Seven kinds of ginseng disease target bacteria (ginseng rust fungus ( Cylindrocarpon destructans ), Botrytis ginseng ( Botrytis cinerea ), Sclerotinia ginseng ( Sclerotinia schinseng ), Phytophthora ginseng ( Phytophthora cactorum ), Panax ginseng ( Alternaria panax ), ginseng root rot fungus ( Fusarium solani ), Panax ginseng ( Rhizoetonia solani )) Bacteria cakes were inserted into the center of the PDA culture dish, and endophytic bacteria NJ13 were streaked into both sides of the target bacteria at the same time, and cultured at 25°C for 2-4 days. The endogenous antagonistic bacteria NJ13 of ginseng had antibacterial effects on the above diseases ( figure 1 ).

[0026] The antimicrobial spectrum of ginseng endogeno...

Embodiment 3

[0029] Example 3: Identification of ginseng endogenous antagonistic bacteria NJ13

[0030] Streak inoculation of NJ13 strain on NA medium, culture at 28°C for 18-24 h, observe and record single colony morphology. NJ13 showed pale yellow opaque colonies on NA medium, with rough surface and wrinkled edges, irregular edges, biofilm formation in liquid static culture, positive Gram staining, and motility ( image 3 ).

[0031] Take the NJ13 strain cultured in LB liquid medium for 24 h for negative staining to prepare samples, clamp the copper mesh with film with tweezers to dip in a small amount of bacterial liquid, let it stand for 2 min, and blot the remaining liquid from the edge with filter paper; Then use tweezers to clamp the bacteria-carrying copper mesh and immerse it in a beaker filled with double distilled water for rinsing, then blot the liquid with filter paper, and then negatively stain with 2% phosphotungstic acid for 5 min. Rod-shaped, with perinatal flagella, can...

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Abstract

The invention discloses preparation and application of a biological control bacterial strain for diseases of a ginseng plant. The biological control bacterial strain is a ginseng endogenous antagonistic bacterium NJ13, namely bacillusmethylotrophicus which is separated and screened from stems of the ginseng plant; the zymocyte liquor and filter liquor of the bacillusmethylotrophicus can antagonize seven common diseases of the ginseng plant and other common plant diseases, and especially have a better control effect on botrytis and black spots of the ginseng plant; the bacillusmethylotrophicus is derived from the ginseng plant and is safe to the ginseng plant; raw materials in the fermentation process of the bacillusmethylotrophicus are low in cost; an antimicrobial active material is simple to prepare, convenient to operate, and good in control effect; and the bacillusmethylotrophicus is a broad-spectrum and efficient green biological pesticide which can be subjected to industrialized production, and can effectively solve the problem of serious residues of pesticides for disease control of the ginseng plant.

Description

technical field [0001] The invention belongs to the technical field of plant biological control, and in particular relates to the control of plants, in particular to a methylotrophic bacillus ( Bacillus methylotrophicus ) and applications. Background technique [0002] Ginseng is a kind of precious Chinese medicinal material, which has a long history of medicinal use in my country. In recent years, due to the growth of market demand, the scale of artificial cultivation of ginseng has also increased. During the ginseng planting process, the occurrence and development of diseases will have a serious impact on yield and quality, and effective disease control is an important link in ginseng production. At present, the main method of disease prevention and control is to use chemical pesticides to control the disease during the disease occurrence period, but long-term use of chemical pesticides or irrational use will lead to excessive pesticide residues in ginseng, quality declin...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00A01P1/00C12R1/07
Inventor 陈长卿姜云尹望
Owner JILIN AGRICULTURAL UNIV
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