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Seed coating agent of Trichoderma fungi and preparation method thereof

A technology of Trichoderma fungi and strains, which is applied in the fields of botanical equipment and methods, fungicides, seed coating/seed dressing, etc., can solve the problems of high cost, poor film-forming performance, poor environmental adaptability, etc. Long term, good film formation and good stability

Active Publication Date: 2013-04-17
SHANGHAI WANLIHUA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, many film-forming materials have poor film-forming performance, poor environmental adaptability, or high cost, and there are still many defects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Fermentation of bacterial strains to prepare bioactive ingredients, the fermentation method can be prepared by traditional solid fermentation or liquid fermentation to prepare conidia, chlamydospores or mycelium, diluted to 1.0×10 spores 6 CFU / ml.

[0054] Take a small amount of water, add 0.5g of Tween 80 and 2g of polyethylene glycol and place it on a magnetic stirrer to stir, then take 4g of glycerin, 1g of xanthan gum and 0.5g of methylcellulose and add them respectively, stir evenly, 2g Continue to stir the water-soluble eosin evenly.

[0055] The seed coating treatment is performed at a volume ratio of 1:20, and the film-forming agent is coated on the seeds, and dried at 35°C for 1-2 hours.

Embodiment 2

[0057] Strain liquid fermentation to prepare chlamydospore fermentation liquid, 3 layers of gauze to filter out mycelium, dilute to Trichoderma chlamydospore digit 1.0×10 6 CFU / ml. The fermentation method is as follows:

[0058] Step 1, the strain is inoculated in the culture medium and cultured at a constant temperature;

[0059] Step 2, transferring the cultured strains to the culture medium for shaking flask culture to obtain conidia liquid;

[0060] Step 3, putting culture liquid into the fermenter, sterilizing under high temperature and high pressure, then pouring the conidia liquid obtained in step 2 into the fermenter, pressurizing and feeding air, and fermenting to obtain chlamydospores;

[0061] Broad Bean Flour: 2wt% glucose: 0.2wt% Yeast extract: 0.4wt% Mixed Fruit and Vegetable Juice: 3wt% pH value: 7.5

[0062] Take a small amount of water, add 1g of Tween 80 and 5g of polyethylene glycol and stir on a magnetic stirre...

Embodiment 3

[0065] Ferment Trichoderma CGMCC No.5203 ​​to prepare chlamydospore fermentation liquid, filter out the mycelia with 3 layers of gauze, and dilute to the number of Trichoderma chlamydospores 1.0×10 6 CFU / ml.

[0066] Take a small amount of water, add 0.5g of Tween 80 and 2g of polyethylene glycol and place it on a magnetic stirrer for stirring, then add 4g of glycerin, 1g of xanthan gum and 0.5g of methylcellulose respectively, stir evenly, and then Add 2g of warning color and continue stirring evenly.

[0067] Coat the cucumber seeds with a volume ratio of 1:20, apply the film-forming agent to the seeds, and dry them at 35°C for 1-2 hours.

[0068] Pythium ( Python spp ), Fusarium ( Fusurium spp ), Rhizoctonia ( Rhizactonia spp. ) will infect the germinating seeds, causing rotten seeds and affecting the germination rate of seeds. Experimental result shows, use the seedling stage root rot control effect of seed coating agent of the present invention to be 91%, measure...

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PUM

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Abstract

The invention discloses a pesticide seed coating agent, a seed coating agent film forming material and a preparation method of the seed coating agent. According to the invention, active chlamydospore of bacteria fermentation broth is added into a film forming material prepared from polyethylene glycol, xanthan gum and methyl cellulose, and then the seeds are wrapped. The seed coating agent is uniform in appearance and not easy to drop, and does not influence budding rate, wherein number of the Trichoderma chlamydospore on each seed is not less than 10<4> CFU; and the seed coating agent can effectively protect seeds in a germination stage.

Description

technical field [0001] The invention relates to a pesticide seed coating agent and a preparation method thereof, in particular to a Trichoderma fungus seed coating agent and a preparation method thereof. Background technique [0002] Seeds are an important guarantee for the high yield and high quality of crops, but the pathogenic bacteria carried by the seeds themselves or the potential pathogens in the soil often affect the germination of the seeds. At present, seed treatment is one of the very important, effective and convenient means of plant protection. Among them, the seed coating agent has the function of using The advantages of convenience, low control cost and long-lasting drug effect are very suitable for large-scale pesticide production. The seed coating agent is coated on the surface of the seed to form a protective layer with a certain strength and permeability. Its preferred ingredients include insecticides, fungicides, micro-fertilizers, plant growth hormones, ...

Claims

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Application Information

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IPC IPC(8): A01N25/00A01N63/04A01P3/00A01C1/06
Inventor 王承芳张钰旷文丰张小明陈娟毛伟力
Owner SHANGHAI WANLIHUA BIOTECH
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