Application of microvesicles from bone marrow mesenchymal stem cells in preparing drug for treating kidney injury

A bone marrow mesenchymal and stem cell technology, applied in the field of stem cell microbubbles, to reduce cell damage, improve kidney tissue damage, and reduce lymphocyte infiltration

Inactive Publication Date: 2013-04-17
JIANGSU PROVINCE HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is not yet clear whether the function of microvesicles to transport gene information plays an important role in stem cell remodeling and tissue regene

Method used

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  • Application of microvesicles from bone marrow mesenchymal stem cells in preparing drug for treating kidney injury
  • Application of microvesicles from bone marrow mesenchymal stem cells in preparing drug for treating kidney injury
  • Application of microvesicles from bone marrow mesenchymal stem cells in preparing drug for treating kidney injury

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Experimental program
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Embodiment 1

[0026] 1. In vitro experiment:

[0027] 1. Culture of human proximal renal tubular epithelial cells (HK2): HK2 was purchased from ATCC, USA. M199 culture solution containing 5% newborn fetal bovine serum and penicillin / streptomycin (100 μg / L) was used to culture in a 37°C incubator (5% CO2), and the medium was changed every 2 days. When the cell density reaches 80%-90%, it is digested with 0.25% trypsin (containing 1 mmol / L EDTA) and subcultured at 1:2-3, which is designated as P1. Continue to subculture according to the above method, and inoculate according to the cell concentration required for the detection, replace the serum-free culture medium, and after 24 hours of synchronization, change to fresh serum-containing culture medium. Add TGF-β1 (6ng / ml), MV (30μg / ml), MSC (1×10 5) After 48h and 72h respectively, observe.

[0028] 2. Grouping:

[0029] (1) HK-2 control group

[0030] (2) HK-2+TGF-β1

[0031] (3) HK-2+TGF-β1+MSC

[0032] (4) HK-2+TGF-β1+MV

[0033] 3. Ob...

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Abstract

The invention discloses application of microvesicles from bone marrow mesenchymal stem cells (MSCs) in preparing a drug for treating a kidney injury. According to the application, a recovery effect of the MVs (microvesicles) in a kidney fibration process is verified by in vivo experiments and in vitro experiments. Fibration of renal tubular epithelial cells is caused by TGF-beta1 in the in vitro experiments, the research indicates that the extends of a cell fibration lesion and a cell injury in an MV group are obviously reduced, a unilateral ureteral occlusion animal model is built, the research indicates that in an UUO (unilateral ureteral occlusion) mouse model, average levels of SCr (serum creatinine ), blood UA ( uric acid), urinary protein of an UUO mouse injected with the MVs fall remarkably, the further pathological observation indicates that the extent of the kidney tissue injury of the mouse is improved obviously, the extends of renal mesenchymal fibration, lymphocyte infiltration and renal tubular swelling and necrosis are reduced obviously, and the kidney histopathology grade falls remarkably. That the MSC-MVs have a kidney injury recovery effect on the mouse with the unilateral ureteral occlusion is proved.

Description

technical field [0001] The invention belongs to the technical field of stem cell microvesicles, and in particular relates to the application of microvesicles derived from bone marrow mesenchymal stem cells in the preparation of medicines for treating kidney damage. Background technique [0002] The annual incidence rate of chronic renal failure in the natural population is 9.8-19.8 per 100,000 population, and it is increasing at a rate of about 8% per year. Despite people's unremitting efforts to explore the mechanism of chronic renal fibrosis, there is still no very effective method to delay the progression of chronic kidney disease. Therefore, there is an urgent need to find new ways to repair kidney damage and effectively prevent and treat chronic renal failure. [0003] Stem cells are undifferentiated cells, a type of cells with self-renewal, multi-directional differentiation and high proliferation functions. Nowadays, stem cell and regenerative medicine research has b...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P13/12C12N5/0775
Inventor 赵卫红吴剑卿何娟朱蓓孙姝裴小华
Owner JIANGSU PROVINCE HOSPITAL
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