Alpha-galactosidase, and coding gene and application thereof

A galactosidase and gene technology, which is applied to α-galactosidase and its encoding gene and application fields, can solve the problems of few research reports on α-galactosidase, and achieve great application potential and good thermal stability. Effect
CN103045562AActive Publication Date: 2013-04-17CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2013-04-17

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Abstract

The invention discloses an alpha-galactosidase, and a coding gene and application thereof. The protein provided by the invention is (a) a protein composed of amino acid residues from 24 site to 751<st> site at the N terminal in Sequence 1 in a sequence table; or (b) a protein composed of amino acid sequences shown in Sequence 1 in the sequence table; or (c) a protein which has alpha-galactosidase activity, is derived from Sequence 1 and is formed by the amino acid sequence of (a) or (b) through the substitution and / or deletion and / or addition of one or more amino acid residues. The protein provided by the invention, serving as alpha-galactosidase, has the following enzymatic properties: the optimum pH value is 4.5, and the protein is stable in the pH value range of 4-10; and the optimum temperature is 60 DEG C, the protein is stable within 55 DEG C, the enzyme activity hardly changes when the protein is kept at 55 DEG C for 30 minutes, and 70% of the activity can be remained when the protein is kept at 60 DEG C for 30 minutes, thus indicating favorable heat stability. The protein provided by the invention has great potentials in industrial application.
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Description

technical field

[0001] The invention relates to an alpha-galactosidase, its coding gene and application. Background technique

[0002] α-galactosidase (EC 3.2.1.22, α-galactosidase), also known as melibiase, belongs to the exoglycosidase class, which can specifically hydrolyze the α-galactosidic bond at the non-reducing end of the sugar chain, and is widely used In food processing, feed industry and medicine and other fields. In the food industry, α-galactosidase can be used to prepare soybean milk products with low α-galactosyl oligosaccharide content, which is beneficial to human digestion. In the feed industry, adding α-galactosidase can not only degrade oligosaccharides and improve the metabolic energy of soybean meal, but also eliminate intestinal flatulence and increase animal feed intake. In medicine, using the transferase activity of α-galactosidase, cyclodextrin can be modified to change the physical and chemical properties of the drug, increase the stability of t...

Claims

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