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Method for preparing bio-ethanol with suaeda salsa as raw material

A technology of bioethanol and Suaeda salsa, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as inconsistency in the optimum action temperature, achieve short production cycles, improve synchronous saccharification and fermentation efficiency, and ethanol output Improved effect

Active Publication Date: 2013-04-17
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to provide a method for preparing bioethanol with Suaeda salsa as raw material, to solve the key problem that the optimal temperature of yeast and cellulase in synchronous saccharification and fermentation to produce ethanol is inconsistent, and to improve Simultaneous fermentation efficiency

Method used

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  • Method for preparing bio-ethanol with suaeda salsa as raw material
  • Method for preparing bio-ethanol with suaeda salsa as raw material

Examples

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Effect test

Embodiment 1

[0029] The application of embodiment 1 normal temperature cellulase

[0030] A method for preparing bioethanol with Suaeda salsa as raw material, the specific steps are as follows:

[0031] (1) Pre-treatment of Suaeda salsa stalks: ① Collect dead stalks of Suaeda salsa and remove impurities; ② Dry the stalks after ① removal of impurities; The diameter is 0.45-0.9mm;

[0032] (2) Pretreatment of Suaeda stalk: mix 800g of the treated stalk with 0.7% (w / w) sodium hydroxide solution at a solid-liquid ratio of 1:15 (g / ml), Treat at 110°C for 10 minutes;

[0033] (3) Preparation of Suaeda stalk fermentation medium: Adjust the pH value of the pretreated Suaeda stalk feed liquid to 5.0, and add yeast powder with a final concentration (w / v) of 0.3%, 0.5% peptone, 0.02% urea, 0.4% magnesium sulfate, 0.01% diammonium phosphate;

[0034] (4) Simultaneous saccharification and fermentation to produce ethanol: add 8% high-activity Saccharomyces cerevisiae with a mass ratio of 8% to the f...

Embodiment 2

[0037] Application of embodiment 2 Antarctic low temperature cellulase

[0038] A method for preparing bioethanol with Suaeda salsa as raw material, the specific steps are as follows:

[0039] (1) Pre-treatment of Suaeda stalks: ① Collect dried stalks and remove impurities; ② Dry ① the stalks after removal of impurities; ③ Grind the stalks after treatment to particle size 0.45—0.9mm;

[0040] (2) Pretreatment of Suaeda stalk: mix 800g of the treated stalk with 0.7% (w / w) sodium hydroxide solution at a solid-liquid ratio of 1:15 (g / ml), Treat at 110°C for 10 minutes;

[0041] (3) Preparation of Antarctic low-temperature enzyme: ① Inoculate and activate the Antarctic low-temperature bacterial strain QP7 screened from Antarctic soil samples preserved in the laboratory; In the enzyme medium, the medium consists of (NH4) 2 SO 4 0.5% (g / ml), MgSO 4 0.02% (g / ml), bran 3% (g / ml), cultured on a low-temperature shaker at 150rpm, 15°C for 4 days; ③ Centrifuge the QP7 enzyme-producing...

Embodiment 3

[0046] Example 3 Combined application of low temperature cellulase and normal temperature cellulase

[0047] A method for preparing bioethanol with Suaeda salsa as raw material, its technical process is shown in figure 1 ,Specific steps are as follows:

[0048] (1) Pre-treatment of Suaeda stalks: ①Collect dry stalks and remove impurities; ②Dry the ①Salsa stalks after removal of impurities; 0.45—0.9mm;

[0049] (2) Pretreatment of Suaeda stalk: mix 800g of the treated stalk with 0.7% (w / w) sodium hydroxide solution at a solid-liquid ratio of 1:15 (g / ml), Treat at 110°C for 10 minutes;

[0050] (3) Preparation of Antarctic low-temperature cellulase: ① Inoculate and activate the Antarctic low-temperature bacterial strain QP7 screened from Antarctic soil samples preserved in the laboratory; In the enzyme production medium, the medium consists of (NH4) 2 SO 4 0.5% (g / ml), MgSO 4 0.02% (g / ml), bran 3% (g / ml), cultured on a low-temperature shaker at 150rpm, 15°C for 4 days; ③...

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Abstract

The invention relates to the field of energy, and discloses a method for preparing bio-ethanol with suaeda salsa as a raw material. The method comprises the steps of: (1) collecting suaeda salsa stalks, removing impurity, crushing and other pre-treatment; (2) mixing the processed suaeda salsa stalks with a sodium hydroxide solution, preprocessing at 100-115 DEG C for 10-30min; (3) activating the Antarctic low temperature strain, inoculating the strain in a bran enzyme-production medium, and culturing to obtain a low temperature enzyme; (4) adjusting the pH of the suaeda salsa stalk pretreatment liquid to 4.5-5.5, adding the low temperature enzyme, a normal temperature cellulase enzyme or a complex enzyme of both according to a certain amount, inoculating 8% of a yeast activation liquid, conducting simultaneous saccharification and fermentation under certain conditions; and (5) distilling the fermented liquid to obtain high purity bio-ethanol. The method utilizes the suaeda salsa stalk as the raw material for preparing bio-ethanol, is simple and feasible in process, achieves efficient resource utilization for suaeda salsa, has a certain economic benefit, applies the Antarctic low temperature enzyme in simultaneous saccharification and fermentation, and helps to improve the fermentation effects.

Description

technical field [0001] The invention belongs to the field of biomass energy, and relates to a method for preparing bioethanol, in particular to a technical method for preparing bioethanol from saline-alkali land plant Suaeda stalks as raw materials, and especially relates to the production of Antarctic low-temperature cellulase on Suaeda stalks Application of simultaneous saccharification and fermentation to produce ethanol. Background technique [0002] With the depletion of fossil resources and the worsening of environmental pollution, countries all over the world are paying attention to the continuous search for new green renewable resources. Among them, the development and production of bioethanol using biomass as raw material is a research hotspot in the field of energy today, and has become the world's It is one of the important subjects of research and promotion in various countries. At present, in my country, under the principle of "not competing with others for foo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCY02E50/10
Inventor 王能飞明凯利其他发明人请求不公开姓名
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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