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Method for improving L-arginine fermentation yield and saccharic acid transformation rate

A sugar-acid conversion rate, arginine technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the gap in industrial production, the limited level of arginine fermentation, the gap in L-arginine, etc. problem, to achieve the effect of simple and easy method

Active Publication Date: 2013-04-24
FUJIAN GUTIAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are often aimed at modifying a single gene or improving the activity of a certain reaction enzyme, and there is still a big gap between improving the fermentation level of L-arginine and applying it to actual production.
In recent years, a small number of studies have also shown that a single addition of an appropriate amount of amino acids, organic acids, and vitamins in the fermentation medium can promote the fermentation of L-arginine to a certain extent, but these studies only stop at the addition of a single factor in the experiment. The impact on improving the level of arginine fermentation is also extremely limited, and the fermentation of L-arginine in these studies is at a low level, and there is still a large gap in the realization of industrialized production

Method used

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  • Method for improving L-arginine fermentation yield and saccharic acid transformation rate
  • Method for improving L-arginine fermentation yield and saccharic acid transformation rate
  • Method for improving L-arginine fermentation yield and saccharic acid transformation rate

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The fermentation medium is: glucose 80.0-100.0 g / L, corn steep liquor 10.0-40.0 g / L, (NH 4 ) 2 S0 4 5.0~40.0g / L,KH 2 P0 4 0.5~2.0g / L,K 2 HP0 4 ·3H 2 0 0.1~1.5 g / L, MgS0 4 ·7H 20 0.1 ~ 1.5 g / L, initial pH 7.0 ~ 7.2; Add any of the following groups of fermentation cofactors to the fermentation medium to conduct experiments:

[0027] Cofactor 1: 0.1×10 -4 g / L Thiamine, 0.1×10 -4 g / L riboflavin, 1.5×10 -4 g / L Niacin, 0.1×10 -4 g / L pyridoxine, 0.3×10 -4 g / L biotin, 0.2×10 -5 g / L folic acid, 0.1×10 -4 g / L pantothenic acid.

[0028] Cofactor 2: 1×10 -4 g / L Thiamine, 0.2×10 -4 g / L riboflavin, 2×10 -4 g / L Niacin, 0.6×10 -4 g / L pyridoxine, 0.6×10 -4 g / L biotin, 0.4×10 -5 g / L folic acid, 1.5×10 -4 g / L pantothenic acid.

[0029] Cofactor 3: 1×10 -4 g / L Thiamine, 0.5×10 -4 g / L riboflavin, 2×10 -4 g / L Niacin, 0.6×10 -4 g / L pyridoxine, 0.9×10 -4 g / L biotin, 0.4×10 -4 g / L folic acid, 2.5×10 -4 g / L pantothenic acid.

[0030] Cofactor 4: 3×10 -4 g / L Thia...

Embodiment 2

[0034] Fermentation medium: glucose 80.0-100.0 g / L, corn steep liquor 10.0-40.0 g / L, (NH 4 ) 2 S0 4 5.0~40.0g / L,KH 2 P0 4 0.5~2.0g / L,K 2 HP0 4 ·3H 2 0 0.1~1.5 g / L, MgS0 4 ·7H 2 0 0.1-1.5 g / L, initial pH 7.0-7.2.

[0035] Put the Brevibacterium flavum liquid in the logarithmic phase into the 10L automatic control fermenter containing the fermentation medium according to the inoculation amount of 10%, the initial constant volume is 5L, the temperature is controlled at 30°C, and proper air is introduced, and the stirring speed is adjusted. Control the dissolved oxygen at 20% to 50%, control the pH at 7.0 to 7.2 by automatically feeding ammonia water, and control the residual sugar at 1.0% by feeding an appropriate amount of foam to defoam, and by feeding a glucose solution with a concentration of 800g / L . The feeding of fermentation cofactors was started when the fermentation reached 12 hours, and the feeding was stopped when the fermentation reached 45 hours. The fl...

Embodiment 3

[0042] Fermentation medium: glucose 80.0-100.0 g / L, corn steep liquor 10.0-40.0 g / L, (NH 4 ) 2 S0 4 5.0~40.0g / L,KH 2 P0 4 0.5~2.0g / L,K 2 HP0 4 ·3H 2 0 0.1~1.5 g / L, MgS0 4 ·7H 2 0 0.1-1.5 g / L, initial pH 7.0-7.2.

[0043] Put the Brevibacterium flavum liquid in the logarithmic phase into the 10L automatic control fermenter containing the fermentation medium according to the inoculation amount of 10%, the initial constant volume is 5L, the temperature is controlled at 30°C, and proper air is introduced, and the stirring speed is adjusted. Control the dissolved oxygen at 20% to 50%, control the pH at 7.0 to 7.2 by automatically feeding ammonia water, and control the residual sugar at 1.0% by feeding an appropriate amount of foam to defoam, and by feeding a glucose solution with a concentration of 800g / L . During the fermentation process, the fermentation cofactors are added in one of the following ways.

[0044] Fed-batch method 1: Add thiamine, riboflavin, niacin, p...

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Abstract

The invention discloses a method for improving an L-arginine fermentation yield and a saccharic acid transformation rate. The method comprises the main steps of: fermenting an L-arginine producing strain to obtain L-arginine; and adding fermentation cofactors into a fermentation medium at the beginning or middle process of the fermentation, wherein according to the total volume of the fermentation medium, the fermentation cofactors include 0.01*10-4 to 10*10-4 g / L of thiamine, 0.01*10-4 to 2*10-4 g / L of riboflavin, 0.1*10-4 to 10*10-4 g / L of niacin, 0.01*10-4 to 9*10-4 g / L of pyridoxine, 0.01*10-4 to 5*10-4 g / L of biotin, 0.05*10-5 to 6*10-6 g / L of folic acid, and 0.01*10-4 to 10*10-4 g / L of pantothenic acid. Without adding an external device and extra human input, the method for improving the L-arginine fermentation yield and the saccharic acid transformation rate shortens fermentation production period of the L-arginine, greatly improves the yield as well as the transformation rate, is simple and easy, and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of amino acid production by fermentation, and in particular relates to a method for improving the fermentation yield of L-arginine and the conversion rate of sugar and acid. Background technique [0002] L-arginine is a semi-essential amino acid in humans and animals, not only a component of body proteins, but also a synthetic precursor of various biologically active substances. Arginine participates in the urea cycle and is an important intermediate metabolite of the urea cycle in organisms; arginine also affects the cardiovascular system, nervous system and immune system through the nitric oxide (NO) pathway; arginine plays a role in regulating reproductive function important role in gene expression. In starvation, trauma or emergency conditions, L-arginine becomes an essential amino acid. With the continuous in-depth research and understanding of the biological functions of arginine, arginine has become...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/10C12R1/15C12R1/13
Inventor 李少平周旭波庄楚周
Owner FUJIAN GUTIAN PHARMA