Culture method for microalgae

A technology for culturing and cultivating microalgae, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as pollution control of open cultivation of microalgae, and achieve the solution of pollution, disease and insect pest control, and improvement of astaxanthin. , the effect of increasing the growth rate

Active Publication Date: 2013-05-15
CHINA PETROLEUM & CHEM CORP +1
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the present invention provides a microalgae cultivation method, which can solve the problem of pollution control in the open cultivation of micr

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The control effect of the extract on the contamination of miscellaneous bacteria and pests during the cultivation process.

[0021] Inoculate the chlorella algae liquid in the logarithmic growth phase in a 20L open raceway pool reactor, the medium is SE medium, and the initial density of chlorella after inoculation is 1 × 10 6 Individual / ml, culture experiments were carried out with different contents of plant extracts. The experiment was carried out at 25°C, the light was 3000lux, the light-dark cycle was 24h, and the light-dark time ratio was 14:10 (hours). After 6 days of cultivation, the number of Chlorella and bacteria were observed under the microscope by blood counting method. The results are shown in Table 1.

[0022] Table 1 Monitoring results of Chlorella contamination by adding different plant extracts

[0023] Extract type Extract content (mg / L) Chlorella biomass (unit / ml) The number of cells of miscellaneous bacteria and pests (unit / ml) ...

Embodiment 2

[0026] Effects of different concentrations of plant extracts on the biomass and oil content of microalgae.

[0027] Inoculate the Botrytis brownii liquid in the logarithmic growth phase in a 10L light reactor, using Chu10 medium, and the initial density of the culture medium after inoculation is 1.1×10 6 each / ml, respectively add different extracts to the culture solution for cultivation, after 20 days of cultivation, the growth of Botrytis is in a stable phase, the algae liquid is collected by centrifugation and dried to obtain dry algae powder, and the respective biomass is obtained by weighing, and The oil content was determined by Soxhlet extraction. The results obtained are shown in Table 2. As can be seen from the results in Table 2, adding garlic extract can significantly improve the final biomass and oil content of Botrytis, the preferred concentration of garlic extract is 60mg / L, the biomass increases by 11%, and the fat content increases by 12%, while Capsicum and ...

Embodiment 3

[0031] Effects of plant extracts on the production of astaxanthin from Haematococcus yussinus.

[0032] Inoculate the Haematococcus lycoccus liquid in the logarithmic growth phase in a 10L light reactor, using SE medium, and the initial density of the culture medium after inoculation is 1.15×10 6 each / ml, different extracts were added to the culture solution for cultivation, and the Haematococcus yushengi liquid was harvested after 30 days of cultivation, and the astaxanthin content was determined. The obtained results are shown in Table 3. As can be seen from Table 3, adding garlic extract can significantly increase the content of astaxanthin, the preferred concentration of garlic extract is 60mg / L, at this time the output of astaxanthin has increased by about 19.23%, while pepper and peach leaf extract The effect on the yield of astaxanthin was not significant.

[0033] Table 3 The yield of Haematococcus yussonii with different extracts

[0034] Extract type Ex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a culture method for microalgae. According to the invention, a conventional culture method and conventional culture conditions are employed; in an initial culture stage of microalgae, a plant extract is added in a microalgae culture medium, the plant extract is one or more selected from the group consisting of a garlic extract, a capsicum extract and a peach leaf extract, and the addition amount of the plant extract in the culture medium is 10 to 100 mg/L. The method provided by the invention can overcome problems in pollution treatment in open culture of microalgae, and the method has the advantages of a low price, safety, reliability and capacity of effectively preventing and treating pollution by mixed fungi and insect diseases and promoting growth of microbes and output of a product of value.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a cultivation method for preventing and controlling microalgae pollution. Background technique [0002] Microalgae (Chlorella vulgar) has a comprehensive and balanced nutrition, rich in protein, fatty acids, various pigments, B vitamins, amino acids, trace elements and some biologically active metabolites, and also has anti-cancer, anti-radiation, anti-infection, anti-oxidation, A series of physiological health functions such as prevention and treatment of hyperlipidemia, prevention of constipation and bone marrow suppression are widely used in food, health care products, medicine and energy, and have huge market potential and extraordinary social value. [0003] At present, the large-scale culture of microalgae usually adopts the traditional open large pond culture, which has low production cost, but the open culture is easily polluted by harmful organisms suc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/12C12R1/89
Inventor 师文静张霖李晓姝吴丹王领民高大成王崇辉
Owner CHINA PETROLEUM & CHEM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap