Method for preparation of flavone by culturing radix puerariae cells

A cell and flavonoid technology, applied in the field of cell engineering, can solve the problems of incomplete extraction, unstable flavonoid yield, loss of flavonoid components, etc.

Active Publication Date: 2013-06-12
浙江华缔药业集团医药开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many methods for extracting flavonoids, such as hot water extraction, lye extraction, acetone extraction, ethanol extraction, microwave extraction, ultrasonic method, etc., but the commonality of these methods is that the flavonoid-containing pl

Method used

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  • Method for preparation of flavone by culturing radix puerariae cells
  • Method for preparation of flavone by culturing radix puerariae cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Preparation of flavonoids by culturing kudzu root cells

[0035] 1. Prepare medium (mass percentage concentration)

[0036] Formulated with 8.25% NH 4 NO 3 , 9.50% KNO 3 , 1.85% MgSO 4 ·7H 2 The first solution that O and surplus water form;

[0037] Formulated with 2.51% CaCl 2 2H 2 O and the second solution that surplus water forms;

[0038] Formulated with 0.85% KH 2 PO 4 And the third solution that surplus water forms;

[0039] Formulated with 0.139% FeSO 4 ·7H 2 O, 0.187% EDTA-Na 2 And the fourth solution that surplus water forms;

[0040] Prepared by 0.00415% KI, 0.00012% CoCl 2 ·6H 2 O, 0.031% H 3 BO 3 , 0.00125% Na 2 MoO 2 2H 2 O, 0.0845% MnSO 4 ·H 2 O, 0.00012% CuSO 4 ·5H 2 O, 0.043% ZnSO 4 ·7H 2 The 5th solution that O and surplus water form;

[0041] Prepare the sixth solution consisting of 0.5% inositol, 0.0025% niacin, 0.0025% pyridoxine hydrochloride, 0.0005% thiamine hydrochloride, 0.01% glycine and surplus water; ...

Embodiment 2

[0049] Embodiment 2: Preparation of flavonoids by culturing kudzu root cells

[0050] 1. Prepare medium (mass percentage concentration)

[0051] Formulated with 8.25% NH 4 NO 3 , 9.50% KNO 3 , 1.85% MgSO 4 ·7H 2 The first solution that O and surplus water form;

[0052] Formulated with 2.51% CaCl 2 2H 2 O and the second solution that surplus water forms;

[0053] Formulated with 0.85% KH 2 PO 4 And the third solution that surplus water forms;

[0054] Formulated with 0.139% FeSO 4 ·7H 2 O, 0.187% EDTA-Na 2 And the fourth solution that surplus water forms;

[0055] Prepared by 0.00415% KI, 0.00012% CoCl 2 ·6H 2 O, 0.031% H 3 BO 3 , 0.00125% Na 2 MoO 2 2H 2 O, 0.0845% MnSO 4 ·H 2 O, 0.00012% CuSO 4 ·5H 2 O, 0.043% ZnSO 4 ·7H 2 The 5th solution that O and surplus water form;

[0056] Prepare the sixth solution consisting of 0.5% inositol, 0.0025% niacin, 0.0025% pyridoxine hydrochloride, 0.0005% thiamine hydrochloride, 0.01% glycine and surplus water; ...

Embodiment 3

[0064] Embodiment 3: Preparation of flavonoids by culturing kudzu root cells

[0065] 1. Prepare medium (mass percentage concentration)

[0066] Formulated with 8.25% NH 4 NO 3 , 9.50% KNO 3 , 1.85% MgSO 4 ·7H 2 The first solution that O and surplus water form;

[0067] Formulated with 2.51% CaCl 2 2H 2 O and the second solution that surplus water forms;

[0068] Formulated with 0.85% KH 2 PO 4 And the third solution that surplus water forms;

[0069] Formulated with 0.139% FeSO 4 ·7H 2 O, 0.187% EDTA-Na 2 And the fourth solution that surplus water forms;

[0070] Prepared by 0.00415% KI, 0.00012% CoCl 2 ·6H 2 O, 0.031% H 3 BO 3 , 0.00125% Na 2 MoO 2 2H 2 O, 0.0845% MnSO 4 ·H 2 O, 0.00012% CuSO 4 ·5H 2 O, 0.043% ZnSO 4 ·7H 2 The 5th solution that O and surplus water form;

[0071] Prepare the sixth solution consisting of 0.5% inositol, 0.0025% niacin, 0.0025% pyridoxine hydrochloride, 0.0005% thiamine hydrochloride, 0.01% glycine and surplus water;

...

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Abstract

Relating to the field of cell engineering, the invention specifically discloses a method for preparation of flavone by culturing radix puerariae cells. The method includes: inoculating radix puerariae callus cells to a culture medium, at a temperature of 23-27DEG C and under illumination intensity of 800-1200lx, carrying out culture for 15-20d in terms of 8-12h of illumination per day, and then performing separation so as to obtain the flavones. By means of optimizing culture conditions and selecting an excellent culture system, the method provided in the invention employs the biotechnological means of culturing radix puerariae cells to prepare flavone, and the yield is substantially higher than the flavones yield of ethanol extraction method, water extraction method and other conventional methods. Thus, a new approach for flavone preparation is opened up.

Description

technical field [0001] The invention relates to the field of cell engineering, in particular to a method for preparing flavonoids by culturing kudzu root cells. Background technique [0002] Flavonoids are a large class of compounds produced by plants through photosynthesis, and their structural core is based on C 6 -C 3 -C 6 As the skeleton of 2-phenyl chromone. It exists in two forms, one is free aglycone, and the other is glycoside combined with sugar. Flavonoids are widely distributed in the plant kingdom, such as ginkgo biloba, mountain ballast, seabuckthorn, rosehip, jusi, eucommia, tea, kudzu root, etc. So far, more than 500 kinds of plants have been found to contain flavonoids and isoflavones, including flavanones, isoflavones, flavonols, flavanone alcohols, biflavones and their derivatives. The main functions of flavonoids are: anti-tumor, anti-aging, enhancing cardiovascular function, treating chronic prostatitis, enhancing immunity, mediating endocrine system...

Claims

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Application Information

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IPC IPC(8): C12N5/04A61K36/488A61P35/00A61P39/06A61P9/00A61P13/08A61P37/04A61P5/00A61P1/16A61P29/00A61P37/08A61P31/04A61P31/12
Inventor 周天琼周正兵高留根
Owner 浙江华缔药业集团医药开发有限公司
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