Streptomycetes silaceus carried on Shenzhou 9 spaceship and space microbial fertilizer prepared from streptomycetes silaceus

A technology of microbial fertilizer, Streptomyces silicium, applied in the directions of microorganisms, microorganism-based methods, organic fertilizers, etc., to achieve the effect of solving nitrogen fixation, good yield increase effect and yield improvement

Active Publication Date: 2013-06-19
刘璐 +4
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention effectively solves the technical problems in the fermentation of existing microbial compound fertilizers, comprehensively solves the effects of nitrogen fixation, phosphorus decomposing and potassium decomposing, and is convenient for farmers, simple and practical

Method used

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  • Streptomycetes silaceus carried on Shenzhou 9 spaceship and space microbial fertilizer prepared from streptomycetes silaceus
  • Streptomycetes silaceus carried on Shenzhou 9 spaceship and space microbial fertilizer prepared from streptomycetes silaceus
  • Streptomycetes silaceus carried on Shenzhou 9 spaceship and space microbial fertilizer prepared from streptomycetes silaceus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment one: Streptomyces silica ( Streptomyces silaceus )SCXL-S9 CGMCC No.7256 screening, classification and identification of

[0034] Obtained by isolation, screening, and domestication from the perennial root of alfalfa planting area in Xinjiang for many years, after optimization, the strain number is SCXL-S9, and the strain is suitable for Gaoshi No. 1 medium: KNO 3 1 g, NaCl 0.5 g, soluble starch 20 g, MgSO 4 7 hours 2 O 0.5 g, K 2 HPO 4 0.5 g, FeSO 4 7 hours 2 O 0.01 g, 20 g of agar, 1000 ml of distilled water. Culture conditions: pH7.2-7.4, temperature 28°C-30°C. Cultivation time: 5-6 days, the colony is round, with smooth edges, milky white aerial mycelia, light yellow basal mycelia, light pink spores, producing apricot yellow soluble pigment; according to the colony morphology, physiology and biochemistry of the strain characteristics, combined with the taxonomic evolution analysis of this strain, the biological taxonomic name is Streptomyc...

Embodiment 2

[0037] Example 2: Analysis of Streptomyces silaceus SCXL-S9 carried by the Shenzhou 9 spacecraft

[0038] In the following experiments, the carrying strain was Streptomyces silica ( Streptomyces silaceus ) SCXL-S9 CGMCC No.7256, and the CK strain is a control test strain, that is, Streptomyces siliciformis that was not carried on the Shenzhou 9 spacecraft ( Streptomyces silaceus ) SCXL-S9.

[0039] 1. Colony culture of piggybacked strains

[0040] After the "Shenzhou" spacecraft returned, the surviving spores in the test tubes of each carrying group were washed out gently with sterile physiological saline, centrifuged and washed twice, and then weighed. Appropriately dilute and spread the separation medium plate, incubate at 30°C for more than 72 hours, observe the growth colony morphology, count and convert the survival rate and morphological mutation rate.

[0041] Survival rate (%) = 100% × (number of colonies grown with strains loaded / number of colonies grown withou...

Embodiment 3

[0066] Example 3: Fermentation process of Streptomyces silaceus SCXL-S9 CGMCC No.7256 carried on Shenzhou 9 spacecraft

[0067] 1. Seed cultivation

[0068] (1) Inoculate the slant culture medium with spores of Streptomyces LS462 and culture at 28°C for 8 days (until the aerial hyphae become plump) to obtain the slant strain.

[0069] (2) Fill 100ml of seed medium into a 500ml glass bottle.

[0070] (3) Digging pieces from the slope of step (1) were inoculated into the medium of step (2), and cultured at 28°C and 180rpm for 96 hours to obtain seed liquid.

[0071] (4) Both the slant medium and the seed medium use Gaoshi No. 1 medium: KNO 3 1 g, NaCl 0.5 g, soluble starch 20 g, MgSO 4 7 hours 2 O 0.5 g, K 2 HPO 4 0.5 g, FeSO 4 7 hours 2 O 0.01 g, 20 g of agar, 1000 ml of distilled water.

[0072] 2. Fermentation culture

[0073] (1) Fill 100ml of fermentation medium in a 500ml Erlenmeyer flask.

[0074] (2) Inoculate 5ml of the seed solution obtained in step 1 ...

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Abstract

The invention discloses a (streptomycetes silaceus) SCXL-S9CGMCC No. 7256 carried on the Shenzhou 9 spaceship and an application of the streptomycetes silaceus in preparation of a space microbial fertilizer. The space microbial fertilizer is prepared by mixing an organic mixture fermentation base stock and a (streptomycetes silaceus) SCXL-S9CGMCC No. 7256 microbial inoculum, wherein the organic mixture fermentation base stock is composed of an organic matter base stock and a decomposed composite microbial inoculum, the organic matter base stock is composed of excrements of livestock, straws and humic acid in a weight ratio of 7:3:2, 1% of decomposed composite microbial inoculum is added to the organic matter base stock according to a weight ratio, and the decomposed composite microbial inoculum is prepared by mixing bacillus subtilis, thermophilic sporotrichum and trichoderma harzianum in a weight ratio of 1:1:1, and 5% of the streptomycetes silaceus is added to the organic mixture base stock for preparing the space microbial fertilizer; and the space microbial fertilizer has an obvious effect on yield increasing of cotton and potato planting and an important application value.

Description

technical field [0001] The present invention relates to the technical field of actinomycetes application, in particular, the present invention relates to the technical field of Streptomyces silica carried on Shenzhou 9 spacecraft and the space microbial fertilizer prepared therefrom. Background technique [0002] The classification of Streptomyces belongs to Bacteria Kingdom, Firmicutes, Actinomycetes, Actinomycetes, Actinomycetes, Streptomyceses, and Streptomycetaceae, and it is a typical representative of Actinomycetes. Streptomyces ( Streptomyces ) is the genus with the largest number of species and the largest number of actinomycetes so far, with more than 500 known species, which are widely distributed in nature. It is widely distributed in soils with low water content, good ventilation, rich in organic matter and slightly alkaline, and also in fresh water, sea water and its silt. A small number of species are pathogenic bacteria of humans, animals and plants. There ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/465
Inventor 唐天成王晓桦唐鹏王春燕张謇汪玲
Owner 刘璐
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