Preparation method of tauro ursodesoxy cholic acid

A technology for tauroursodeoxycholic acid and ursodeoxycholic acid is applied in the field of preparing tauroursodeoxycholic acid, which can solve problems such as environmental pollution and achieve the effects of cheap materials, short reaction time and improved yield.

Active Publication Date: 2013-07-03
山东天绿制药有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantage is that raw materials containing sulfur

Method used

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  • Preparation method of tauro ursodesoxy cholic acid
  • Preparation method of tauro ursodesoxy cholic acid
  • Preparation method of tauro ursodesoxy cholic acid

Examples

Experimental program
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Example Embodiment

[0047] Example 1 Preparation of Tauroursodeoxycholic Acid

[0048] 39.2 g (0.1 mol) of ursodeoxycholic acid was added to 100 ml of N,N-dimethylformamide, and the mixture was stirred to dissolve (the rotation speed was 150 rpm, stirring for 10 minutes). Cool down to 10°C, add 12.66g (0.11mol) of N-hydroxysuccinimide, stir to dissolve (rotate at 135rpm for 20 minutes), add 24.76g (0.12mol) of dicyclohexylcarbodiimide and 4-di 0.12 g (0.001 mol) of methylaminopyridine, after all the materials were added, the reaction was stirred at 12° C. for 2.5 hours at a speed of 135 rpm, and the content of ursodeoxycholic acid detected by HPLC was less than 0.1%, and the reaction was stopped. In addition, 12 g (0.12 mol) of taurine was dissolved in 500 ml of sodium hydroxide aqueous solution (containing 4.8 g of sodium hydroxide, 0.12 mol) to obtain an aqueous solution of taurine sodium salt. The above dimethylformamide solution was added to the taurine sodium salt aqueous solution, and 34ml (0...

Example Embodiment

[0049] Example 2 Preparation of Tauroursodeoxycholic Acid

[0050] 39.2 g (0.1 mol) of ursodeoxycholic acid was added to 100 ml of tetrahydrofuran, and stirred to dissolve (rotating speed 150 rpm, stirring for 10 minutes). Cool down to 0°C, add 12.66g (0.11mol) of N-hydroxyphthalimide, stir to dissolve (rotate at 150rpm and stir for 20 minutes), add 15.14g (0.12mol) of diisopropylcarbodiimide and 0.12 g (0.001 mol) of 4-dimethylaminopyridine. After all the materials were added, the reaction was carried out at 8°C for 20 minutes. The content of ursodeoxycholic acid detected by HPLC was less than 0.1%, and the reaction was stopped. In addition, 12 g (0.12 mol) of taurine was dissolved in 500 ml of sodium hydroxide aqueous solution (containing 4.8 g of sodium hydroxide, 0.12 mol) to obtain an aqueous solution of taurine sodium salt. The above tetrahydrofuran solution was added to the taurine sodium salt aqueous solution, and 34 ml (0.24 mol) of triethylamine was added dropwise. Aft...

Example Embodiment

[0051] Example 3 Preparation of Tauroursodeoxycholic Acid

[0052] 39.2 g (0.1 mol) of ursodeoxycholic acid was added to 100 ml of dimethyl sulfoxide, and the mixture was stirred to dissolve (the rotating speed was 150 rpm and the stirring was for 10 minutes). Cool down to 10°C, add 12.66g (0.11mol) of N-hydroxysuccinimide, stir to dissolve (rotation speed is 150rpm, stir 10), add 1-ethyl-3-(3-dimethylaminopropyl) carbon two 18.62g (0.12mol) of imine and 012g (0.001mol) of 4-dimethylaminopyridine. After all the materials were added, the reaction was stirred at 8°C for 20min at a speed of 150rpm. The content of ursodeoxycholic acid detected by HPLC was less than 0.1% , Stop the reaction. In addition, 12 g (0.12 mol) of taurine was dissolved in 500 ml of sodium hydroxide aqueous solution (containing 4.8 g of sodium hydroxide, 0.12 mol) to obtain an aqueous solution of taurine sodium salt. The above methylene chloride solution was added to the taurine sodium salt aqueous solution,...

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Abstract

The invention discloses a preparation method of tauro ursodesoxy cholic acid. The method comprises the following steps: firstly, dissolving ursodesoxycholic acid in organic solvent, reducing the temperature of the ursodesoxycholic acid to 0 to 10 DEG C, adding amine compounds into the ursodesoxycholic acid, and stirring the ursodesoxycholic acid; secondly, adding condensing agent and 4-dimethylamino pyridine after stirring in the first step, and performing stirring reaction at room temperature, obtaining reaction liquid A; thirdly, adding the reaction liquid A obtained in the second step into sodium taurocholate salt water solution, and dropwise adding triethylamine at room temperature while stirring so as to ensure that the triethylamine is reacted with the reaction liquid A; and fourthly, adding methylene dichloride to perform extraction and liquid separation after the reaction in the third step, then washing a water layer through methylene dichloride, adjusting the pH value after washing, performing concentration after stirring, and then obtaining the tauro ursodesoxy cholic acid. The preparation method has the advantages that the tauro ursodesoxy cholic acid is prepared through a novel technical process, the reaction speed is high, the product quality is high, and raw material is cheap.

Description

technical field [0001] The invention relates to a method for preparing tauroursodeoxycholic acid. Background technique [0002] Tauroursodeoxycholic acid (tauroursodeoxycholic acid) is the active ingredient of bear bile, the chemical name is 2-[[(3α,5β,7β)-3,7-dihydroxy-24-oxocholestan-24-yl ]Amino]ethanesulfonic acid dihydrate, developed by Italy's Besti Pharmaceutical Factory, was first listed in Italy in 1991, and was approved to be sold in China under the trade name taurolite in 2007. Clinically, it is mainly used for the treatment of gallbladder cholesterol stones, primary sclerosing cholangitis, primary biliary cirrhosis and chronic hepatitis C virus. Due to the limitation of natural resources and animal protection, medicinal bezoar ursodeoxycholic acid is mainly obtained by chemical semi-synthesis: [0003] 1. Use ursodeoxycholic acid to react with ethyl chloroformate or pivaloyl chloride to form a mixed anhydride, react with taurine under alkaline conditions, and t...

Claims

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Application Information

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IPC IPC(8): C07J41/00
Inventor 秦笃伟
Owner 山东天绿制药有限公司
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