Swine transmissible gastroenteritis virus S/N protein fusion gene and recombinant lactococcus lactis, and their use

A fusion gene and infectious technology, applied in gene therapy, recombinant DNA technology, DNA / RNA fragments, etc., can solve the problems of strong virulence, uncontrolled expression, and somatic cell canceration, and achieve ideal mucosal immune protection and strengthen the immune system. Immunogenic effects
CN103194471AActive Publication Date: 2013-07-10WUHAN HUAYANG ANIMAL PHARMA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN HUAYANG ANIMAL PHARMA
Publication Date
2013-07-10

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Abstract

The invention discloses a swine transmissible gastroenteritis virus S / N protein fusion gene and recombinant lactococcus lactis, and their use. A and D antigen sites of a S protein of the swine transmissible gastroenteritis virus and an N321 antigen site of an N protein of the swine transmissible gastroenteritis virus are connected to a cell wall anchor sequence of streptococcus pyogenes so that the swine transmissible gastroenteritis virus S / N protein fusion gene is obtained and has a nucleotide sequence shown in the formula SEQ ID No.1. The invention further discloses an expression vector containing the swine transmissible gastroenteritis virus S / N protein fusion gene and the recombinant lactococcus lactis containing the expression vector. The recombinant lactococcus lactis is induced by Nisin so that the swine transmissible gastroenteritis virus S / N protein fusion gene can be expressed on the bacterial cell wall surface. A result of a western blot experiment shows that the expressed recombinant protein can react with TGEV immune serum so that it is proved that the expressed recombinant protein has the antigenicity the same as antigenicity of the TGEV natural antigen and thus the expressed recombinant protein can be prepared into a safe and effective mucosal immune live bacterium vaccine for preventing and treating swine transmissible gastroenteritis.
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Description

technical field

[0001] The present invention relates to an antigen fusion gene and a recombinant strain containing the antigen fusion gene, in particular to a porcine transmissible gastroenteritis virus S / N protein fusion gene and an expression vector containing the fusion gene, and the present invention further relates to a recombinant strain containing the expression vector The invention relates to Lactococcus lactis strains and their use in preparing vaccines for preventing and treating porcine transmissible gastroenteritis, belonging to the field of prevention and treatment of porcine transmissible gastroenteritis. Background technique

[0002] Porcine transmissible gastroenteritis is a highly contagious disease caused by transmissible gastroenteritis virus of swine (TGEV), characterized by severe diarrhea, vomiting, dehydration and high mortality. TGEV mainly exists in the jejunum and duodenum, and pigs of various ages are susceptible. Because piglets have relatively p...

Claims

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