Secondary salinized soil microbial agent and its preparation and use
A technology of secondary salinization and soil microorganisms, which is applied in the field of secondary salinization soil microbial agents, can solve the problems of high cost of soil amendments, slow fertilizer efficiency of semi-decomposed organic fertilizers, and loss of soil nitrogen. Achieve the effects of easy industrial implementation, improved resource utilization, and good repair effects
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Embodiment 1
[0020] Embodiment 1, potted simulation experiment-A
[0021] The soil used for the test is the deep soil of the wasteland on the campus of Minhang Campus of Shanghai Jiaotong University, and the soil type is sandy soil.
[0022] The rice and wheat straws are pulverized into 1-5 mm sieves with a pulverizer. NCT-2 (Bacillus sp.), CB-1, YJ-1 were cultured in a constant temperature incubator using inorganic salt medium to measure their OD 600 =2.38, OD 600 = 2.12, OD 600 =2.29.
[0023] The soil to be tested was air-dried naturally and ground through a 2 mm sieve. The test program is shown in Table 1 below, artificially adding nitrate nitrogen to the soil to make the content reach 800mg / kg. Each pot is filled with 1.0kg of soil, and the bacteria, carrier and sugar source are applied to the shallow surface of the soil at one time, and 30 green vegetable seeds are sown in each pot, placed at room temperature and kept at about 20% soil moisture. On the seventh day, soil sample...
Embodiment 2
[0030] Embodiment 2, potted simulation experiment-B
[0031] The soil used for the test is the deep soil of the wasteland on the campus of Minhang Campus of Shanghai Jiaotong University, and the soil type is sandy soil.
[0032] The rice and wheat straws are pulverized into 1-5 mm sieves with a pulverizer. NCT-2 (Bacillus sp.), CB-1, YJ-1 were cultured in a constant temperature incubator using inorganic salt medium to measure their OD 600 =2.38, OD 600 = 2.12, OD 600 =2.29. The soil to be tested was air-dried naturally and ground through a 2 mm sieve. The test program is shown in Table 3 below, artificially adding nitrate nitrogen to the soil to make the content reach 800mg / kg. Each pot is filled with 1.0kg of soil, and the bacteria, carrier and sugar source are applied to the shallow surface of the soil at one time, and 30 green vegetable seeds are sown in each pot, placed at room temperature and kept at about 20% soil moisture. On the seventh day, soil samples were t...
Embodiment 3
[0040] The preparation of the secondary salinized soil microbial bacterial agent of this embodiment and its use in repairing secondary salinized soil specifically includes the following steps:
[0041] Step 1, cultivation of Bacillus megaterium
[0042] Add 300L potato culture medium and an appropriate amount of defoamer to the 500L fermenter, and after autoclaving the tank (121°C, 45min), ignite alcohol cotton around the inoculation port, and select the cultivated seed liquid with 10% The inoculation amount is connected, and then the inoculation port is covered to enter the cultivation stage. The culture conditions used in the fermenter are: stirring speed 120r / min, culture temperature 30°C, ventilation rate 1.5L / min, pH 7.0-7.2, sampling after 72 hours to determine the concentration of the cultured bacteria solution is 2.0 per milliliter of the bacteria solution. ×10 8 bacteria or more.
[0043] Step 2, the preparation method of secondary salinized soil microbial bacteria...
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