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Enterococcus faecalis and its application in bioelectricity production

A technology of Enterococcus faecalis and strains, applied in the field of electrogenic Enterococcus faecalis, to achieve the effect of reducing requirements and strong electrochemical activity

Active Publication Date: 2013-07-24
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the research of Enterococcus sp. has focused on the field of medical infection, and there is no relevant report on the test of Enterococcus faecalis that can produce electricity in MFCs

Method used

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  • Enterococcus faecalis and its application in bioelectricity production
  • Enterococcus faecalis and its application in bioelectricity production
  • Enterococcus faecalis and its application in bioelectricity production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Screening and Isolation of Strain CCTCCM2012447

[0030] Take 300mL of mangrove bottom mud and transfer it to a 500mL beaker, add 200mL of glucose nutrient solution, stir well, and use NaHCO 3 Adjust the pH value of the solution to about 7.0 (6.7-7.2), and place it in a 30°C incubator for 1 day. Measure the pH and, where appropriate, use NaHCO 3 Adjust the pH of the solution to 6.7-7.2, stir evenly, and discard the supernatant after precipitation. Repeat the culture for 3 days for later use.

[0031] To start a single-chamber air cathode microbial fuel cell, the MFC is a cuboid (length×width×height=8.5×7×6.5cm 3 , effective volume 200mL), the material is 0.5cm plexiglass, one end is closed with a blind plate, and the other end is placed with a splint to place the cathode. The air cathode is platinum-loaded carbon paper (0.5mg / m 2 ), the anode is nitric acid modified carbon felt (BET1200m 2 / g, 2mm), the electrode spacing is 3cm, and titanium wire is used as the el...

Embodiment 2

[0034] Cyclic voltammetry analysis of strain CCTCCM2012445

[0035] Inoculate EnterococcusfaecalisZ5 into high-salt LB medium (recipe: tryptone 10g L -1 , yeast extract 5g·L -1 , sodium chloride 20g·L -1 , pH 7.4), (30±1) ℃ anaerobic culture for 18h. Take the bacterial solution in the mid-logarithmic growth phase, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, and centrifuge the obtained bacterial suspension again according to the above steps, repeat washing twice in this way, and add PBS buffer Make bacterial suspension, OD 600 It is about 0.6. The cyclic voltammetry test was performed on the obtained bacterial suspension with an electrochemical workstation. The test used a three-electrode system, with a glassy carbon electrode as the working electrode, an Ag / AgCl electrode as the reference electrode, and a platinum sheet as the counter electrode. The scan rate was 100mV s -1 , The scanning range is -0.7V~0.7V. See the ex...

Embodiment 3

[0037] Electricity production test of strain CCTCCM2012445 using various artificial wastewater

[0038] The strain CCTCCM2012445 was inoculated into high-salt LB medium (recipe: tryptone 10g L -1 , yeast extract 5g·L -1 , sodium chloride 20g·L -1 , pH 7.4), (30±1) ℃ anaerobic culture for 18h. Take the bacterial solution in the mid-logarithmic growth phase, centrifuge at 5000r / min for 10 minutes, discard the supernatant, add PBS buffer and shake well, and centrifuge the obtained bacterial suspension again according to the above steps, repeat washing twice in this way, and add PBS buffer Make bacterial suspension, OD 600 It is about 0.6.

[0039] Start the single-chamber air cathode microbial fuel cell, the microbial fuel cell is cylindrical (cross-sectional diameter is 2cm, effective volume 6.28cm 3 ), made of polycarbonate. The air cathode is platinum-loaded carbon paper (0.5mg cm -2 ), the anode is nitric acid modified carbon felt, the inside of the cathode is a layer ...

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Abstract

The invention discloses an Enterococcus faecalis strain and its application in bioelectricity production. The strain is characterized in that it is Enterococcus faecalis Z5, which is preserved by China Center for Type Culture Collection (called CCTCC for short) on November 6, 2012 and has a preservation number of CCTCC NO: M2012445. When the strain is inoculated into a microbial fuel cell, it can degrade organic matters and generate electricity simultaneously, but probably does not transmit electrons by secreting its electron transmission intermediates. The Enterococcus faecalis Z5 strain has very strong electrochemical activity, and also can use multiple types of organic matters as the sole carbon source for electricity generation. Based on the above characteristics, the strain has very good application prospects in environmental pollution remediation and bio-energy recovery.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution biological treatment and bioenergy, and specifically relates to an electrogenic Enterococcus faecalis and its application. Background technique [0002] Organic wastewater often contains a large amount of easily degradable organic matter. If these organic matter can be recycled and applied on a large scale, thereby overcoming the original defects of traditional sewage treatment, it will alleviate the problems of energy and environmental water conservation faced by human society. This vision is Due to the rise of microbial fuel cells (MicrobialFuelCells, MFCs), it has gradually become possible. [0003] The so-called microbial fuel cell is a special form of fuel cell. Microbes act as catalysts to convert chemical energy into electrical energy by oxidizing organic or non-organic substances. MFC technology is a new concept of wastewater treatment technology, which uses microorganisms...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20H01M4/90H01M8/16C12R1/46
CPCY02E60/50Y02P70/50
Inventor 朱能武谢海秀吴平霄
Owner SOUTH CHINA UNIV OF TECH
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