Bacillussubtilis for tobacco black shank prevention and control

A technology of Bacillus subtilis and tobacco black shank, applied in the direction of chemicals for biological control, methods based on microorganisms, bacteria, etc., can solve the problem of affecting the control effect, ecological safety, human health, low degree of disease resistance, The content of heavy metals in tobacco leaves exceeds the standard, etc., to achieve potential commercial development and application value, and good prevention and control effects

Inactive Publication Date: 2013-07-31
LIANGSHAN BRANCH OF SICHUAN TOBACCO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to meet the industrial demand for characteristic quality, many tobacco varieties grown in production have a low degree of disease resistance
At present, the control of black shank is still dominated by chemical agents, which leads to the enhancement of pathogenic bacteria resistance, environmental pollution, and excessive heavy metal content in tobacco leaves, which affects the control effect, ecological safety and human health.

Method used

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  • Bacillussubtilis for tobacco black shank prevention and control
  • Bacillussubtilis for tobacco black shank prevention and control
  • Bacillussubtilis for tobacco black shank prevention and control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Acquisition of bacterial strain TBSCQ057 and analysis of related traits

[0026] 1.1 Isolation and purification of strain TBSCQ057: collect healthy tobacco strains, carry out routine surface disinfection treatment on the samples, then peel off the epidermis, cut out the internal tissues and mash them, take the standing solution and apply it on the beef juice peptone plate, select typical colonies, and purify. Then confronted with tobacco black shank bacteria on oat medium to obtain TBSCQ057 strain with strong inhibitory effect on black shank bacteria.

[0027] 1.2 The effect of strain TBSCQ057 on the growth of tobacco seedlings: Inoculate the colony of strain TBSCQ057 stored on the slant medium into NB culture medium for fermentation and culture, obtain a bacterial suspension, and then dilute the bacterial suspension to 3×10 8 cfu / mL. The bacterial suspension was taken for the following 4 treatments: A (seed soaking treatment), B (seed soaking + root irrigat...

example 2

[0031] Example 2. Control effect of bacterial strain TBSCQ057 on black shank

[0032] 2.1 Greenhouse disease control test; scrape off the mycelia of blackleg fungus cultured on oat medium for 14 days, add 0.1% KNO 3 solution infiltration, after sporulation, add sterile water and mash to adjust the concentration to about 1×10 4 sporangia / mL, placed at 8°C for 20 min, diluted 10 times, and added 1% glucose for later use. The TBSCQ057 fermentation broth is obtained by fermentation in a 10-100 L fully automatic fermentation tank for 2 days. Adjust the concentration of the fermentation broth to about 1×10 8 cfu / mL. The pot test used sterilized vegetable soil, put it into a 10 cm×10 cm pot, and poured TBSCQ057 fermentation broth at the time of transplanting and 10 days before inoculation of pathogenic bacteria, 10 mL per plant each time. The control agent (58% nail cream·manganese zinc wettable powder) was used to irrigate the roots with 500 times liquid 24 hours before inocula...

example 3

[0034] Example 3 Fermentation conditions of bacterial strain TBSCQ057

[0035] 3.1 Medium pH value: Use sterile 1mol / L NaOH or HCl to adjust the pH value of one liter of sterilized 30g / L peanut powder culture solution to 4.0, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0 respectively , 9.0 and 11.0 and other 10 treatments, each treatment was repeated 3 times. Inject TBSCQ057 bacterial suspension at 5% inoculum amount, and culture at 28°C and 180 r / min in the dark for 48 h with shaking. After the fermentation broth was diluted by gradient dilution method, count the concentration with a hemocytometer to determine the optimal pH value. .

[0036] 3.2 Cultivation temperature: use 30g / L peanut powder culture solution to cultivate at 22, 25, 28, 31, 34 and 37 °C respectively, insert TBSCQ057 bacterial suspension at 5% inoculum amount, at 28 °C, 180 r / min, Shake culture in the dark for 48 h, and the fermentation broth is diluted by gradient dilution method. The concentration was counted with ...

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Abstract

The invention relates to a strain of Bacillussubtilis TBSCQ057 providing biocontrol activity for tobacco black shank, and belongs to the field of agricultural plant disease biocontrol. The Bacillussubtilis TBSCQ057 is separated from a natural environment host plant, is identified as the Bacillussubtilis according to morphological characteristics and molecular biology, provides a strong inhibition effect for tobacco black shank bacterial, provides a good control effect for tobacco black shank, has broad spectrum antagonism activity, and provides good bacterial inhibition effects for tobacco botrytis cinerea, tobacco alternaria alternate, tobacco sclerotium rolfsii, tobacco anthracnose pathogen, and a lot of pathogenic fungi. Secondary metabolites of the strain contain cellulose, protease, siderophore and the like. The TBSCQ057 bacterial agent provides a good control effect for the tobacco black shank, environmentally friendly bio-pesticides can be produced through bacterial fermentation or a secondary metabolite extraction technology, and important commercial development and application values are provided.

Description

technical field [0001] The invention relates to the technical field of plant disease biological control, in particular to a bacillus subtilis used for preventing and treating tobacco black shank. Background technique [0002] Tobacco is an important economic crop, and tobacco black shank is one of the important diseases in various tobacco growing areas in the world. It occurs in most of the tobacco growing areas in my country, and it is seriously harmful in many provinces and cities. In order to meet the industrial demand for characteristic quality, many tobacco varieties grown in production have a low degree of disease resistance. At present, the control of black shank is still dominated by chemical agents, which lead to the enhancement of pathogenic bacteria resistance, environmental pollution and excessive heavy metal content in tobacco leaves, which affect the control effect, ecological safety and human health. Contents of the invention [0003] The object of the pre...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P3/00C12R1/125
Inventor 王勇邢小军卢军马冠华肖崇刚董国菊
Owner LIANGSHAN BRANCH OF SICHUAN TOBACCO
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