Conduit for tissue-engineered nerve transplanting and preparation method thereof

A nerve transplantation and tissue engineering technology, applied in the field of nerve transplantation catheter and its preparation, can solve problems such as limited application, and achieve the effect of promoting axon growth

Inactive Publication Date: 2013-08-07
NANTONG TEXTILE VOCATIONAL TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely high-speed differential stretching and solidification characteristics of electrospinning, t

Method used

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  • Conduit for tissue-engineered nerve transplanting and preparation method thereof
  • Conduit for tissue-engineered nerve transplanting and preparation method thereof
  • Conduit for tissue-engineered nerve transplanting and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] The preparation method of this specific embodiment is:

[0031] (1) Preparation of oriented nanofiber layer:

[0032] a. Weigh one or more of silk fibroin, collagen, gelatin, laminin and fibrinogen, and dissolve it with one or more solvents of formic acid, hexafluoroisopropanol and hexafluoroacetone to obtain 9- 15% spinning stock solution; can also be synthesized by one or more of polylactic acid (PDLA), polyglycolic acid (PGA) or copolymer of glycolic acid and hydroxypropionic acid (PLGA) with a higher proportion of GA The polymer is dissolved in one or more solvents of 2,2,2-trifluoroethanol, hexafluoroisopropanol, chloroform, dichloromethane, tetrahydrofuran (THF), acetone and dimethylformamide , The formation of 3-8% spinning dope.

[0033] b. The spinning stock solution of step a is sucked into the injection needle tube, the injection needle tube is placed in the micro pump, and the spinning solution feeding speed is controlled by it. The positive electrode of the high...

Example Embodiment

[0040] Example one:

[0041] 1. Weigh 4.4 g of regenerated silk fibroin film and 2.1 g of medical gelatin to dissolve in anhydrous formic acid to obtain 11.5% silk fibroin / gelatin blend spinning solution. Weigh 2 g of polyglycolic acid (PGA) and 1 g of L-polyhydroxypropionic acid (PLLA) and dissolve them in tetrahydrofuran (THF) to form a 4% spinning solution. Dissolve 2.5 g of L-polyhydroxypropionic acid (PLLA) in a mixed solvent of chloroform / acetone (volume ratio 1:1) to obtain 4.9% spinning solution;

[0042] 2. The silk fibroin / gelatin spinning solution prepared in step 1 is sucked into a needle tube, and the needle tube is placed in a micro pump, and the flow rate is adjusted to 0.1 ml / h. The positive pole of the high-voltage generator is connected with the needle, the voltage is 15kV, the negative pole is connected with the metal wires arranged in parallel with the roller, the direction of the metal wire is parallel to the axis of the roller, the diameter of the roller is 1...

Example Embodiment

[0046] Embodiment two:

[0047] 1. Weigh 2 g of racemic polylactic acid (PDLA) and dissolve it in 2,2,2-trifluoroethanol to obtain 3% spinning solution. Weigh 1.8 g of polyglycolic acid (PGA) and 1.2 g of PLGA (LA:GA=60:40), and dissolve them in tetrahydrofuran (THF) to form a 4.2% spinning solution. 2g PLGA (LA:GA=85:15) was dissolved in a mixed solvent of chloroform / acetone (volume ratio 2:1) to obtain 5.0% spinning dope;

[0048] 2. The PDLA spinning stock solution prepared in step 1 is sucked into the needle tube, and the needle tube is placed in the micro pump, and the flow rate is adjusted to 0.13 ml / h. The positive pole of the high voltage generator is connected with the needle, the voltage is 15kV, the negative pole is connected with the metal wires arranged in parallel with the roller, the direction of the metal wires is parallel to the axis of the roller, the diameter of the roller is 10cm, and the speed is 4000rpm. The distance between the needle and the collecting rol...

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Abstract

The invention provides a conduit for tissue-engineered nerve transplanting and a preparation method thereof, and relates to the field of biomedicine application of a high polymer material. The conduit is composed of a plurality of layers of fibrous membranes; the inner fibrous membrane is composed of nanofibers which are arranged in parallel and 40-300nm in diameter; the other fibrous membranes are composed of nano to micro fibers which are irregularly arranged; the degradation velocity of the fibrous membranes is gradually reduced from inside to outside; the degradation velocity of the inner fibrous membrane is 1-2 cycles; the degradation velocity of the outer layer is 6-20 cycles; and a middle layer is arranged between the inner layer and the outer layer. The fiber orientation can be improved; the regularly arranged nanofibers can be rapidly collected; axon growth is guided and promoted; the nerve repair ability is improved; nutrient transportation is facilitated; final finishing of nerve repair can be ensured; and the nerve regeneration speed can be matched with the degradation velocity.

Description

Technical field: [0001] The invention relates to the biomedical application field of polymer materials, in particular to a gradient-degradable nerve graft catheter containing oriented nanofibers and a preparation method thereof. Background technique: [0002] Denervation can lead to sensory and motor dysfunction in the innervated area, affecting the quality of life of patients to varying degrees. Autologous nerve transplantation is the most basic, safe and effective method, and the clinical recovery rate of autologous nerve transplantation can reach 80%. However, autologous transplantation is a secondary operation, with limited sources of donors, difficulty in matching tissue structure and size, long-term denervation of the donor site, and differences in transplantation efficacy, which greatly limit the widespread development of autologous nerve transplantation. [0003] The application of tissue engineering to prepare artificial nerves is an ideal choice. Artificial nerve ...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/18A61L27/24A61L27/22D04H1/74D04H1/728
Inventor 尹桂波陈和春陈桂香郭立强金春奎
Owner NANTONG TEXTILE VOCATIONAL TECH COLLEGE
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