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Conduit for tissue-engineered nerve transplanting and preparation method thereof

A nerve transplantation and tissue engineering technology, applied in the field of nerve transplantation catheter and its preparation, can solve problems such as limited application, and achieve the effect of promoting axon growth

Inactive Publication Date: 2013-08-07
NANTONG TEXTILE VOCATIONAL TECH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely high-speed differential stretching and solidification characteristics of electrospinning, the fibers can only be deposited in a disordered state, which greatly limits its application in neural tissue engineering.

Method used

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  • Conduit for tissue-engineered nerve transplanting and preparation method thereof
  • Conduit for tissue-engineered nerve transplanting and preparation method thereof
  • Conduit for tissue-engineered nerve transplanting and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0030] The preparation method of this specific embodiment is:

[0031] (1) Preparation of oriented nanofiber layer:

[0032]a. Weigh one or more of silk fibroin, collagen, gelatin, laminin and fibrinogen, and dissolve in one or more solvents in formic acid, hexafluoroisopropanol and hexafluoroacetone to obtain 9- 15% spinning dope; it can also be synthesized by one or more of poly-L-lactic acid (PDLA), polyglycolic acid (PGA) or a copolymer of glycolic acid and hydroxypropionic acid (PLGA) with a higher proportion of GA The polymer is dissolved in one or more solvents of 2,2,2-trifluoroethanol, hexafluoroisopropanol, chloroform, dichloromethane, tetrahydrofuran (THF), acetone, and dimethylformamide , forming a 3-8% spinning dope.

[0033] b. Inhale the spinning stock solution in step a into the injection needle, and put the injection needle in the micropump to control the feeding speed of the spinning solution. The positive pole of the high-voltage power generator is connect...

Embodiment 1

[0041] 1. Weigh 4.4g regenerated silk fibroin film and 2.1g medical gelatin and dissolve in anhydrous formic acid to obtain 11.5% silk fibroin / gelatin blended spinning solution. Weigh 2g of polyglycolic acid (PGA) and 1g of L-polyhydroxypropionic acid (PLLA) and dissolve them in tetrahydrofuran (THF) to form a 4% spinning solution. 2.5g of L-polyhydroxypropionic acid (PLLA) was dissolved in chloroform / acetone (volume ratio 1:1) mixed solvent to obtain 4.9% spinning solution;

[0042] 2. Inhale the silk fibroin / gelatin spinning solution prepared in step 1 into a needle tube, put the needle tube in a micropump, and adjust the flow rate to 0.1ml / h. The positive electrode of the high-voltage generator is connected to the needle head, and the voltage is 15kV. The negative electrode is connected to the metal wires arranged in parallel on the rotating roller. The direction of the wire arrangement is parallel to the axial direction of the rotating roller. The distance between the nee...

Embodiment 2

[0047] 1. Weigh 2 g of racemic polylactic acid (PDLA), dissolve it in 2,2,2-trifluoroethanol to obtain a 3% spinning solution. 1.8g of polyglycolic acid (PGA) and 1.2g of PLGA (LA:GA=60:40) were weighed and dissolved in tetrahydrofuran (THF) to form a 4.2% spinning solution. Dissolve 2g of PLGA (LA:GA=85:15) in a mixed solvent of chloroform / acetone (volume ratio 2:1) to obtain a 5.0% spinning dope;

[0048] 2. Inhale the PDLA spinning solution prepared in step 1 into the needle tube, put the needle tube in the micropump, and adjust the flow rate to 0.13ml / h. The positive electrode of the high-voltage generator is connected to the needle head, the voltage is 15kV, the negative electrode is connected to the metal wires arranged in parallel on the rotating roller, the wire arrangement direction is parallel to the axial direction of the rotating roller, the diameter of the rotating roller is 10cm, and the rotating speed is 4000rpm. The distance between the needle and the collecti...

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Abstract

The invention provides a conduit for tissue-engineered nerve transplanting and a preparation method thereof, and relates to the field of biomedicine application of a high polymer material. The conduit is composed of a plurality of layers of fibrous membranes; the inner fibrous membrane is composed of nanofibers which are arranged in parallel and 40-300nm in diameter; the other fibrous membranes are composed of nano to micro fibers which are irregularly arranged; the degradation velocity of the fibrous membranes is gradually reduced from inside to outside; the degradation velocity of the inner fibrous membrane is 1-2 cycles; the degradation velocity of the outer layer is 6-20 cycles; and a middle layer is arranged between the inner layer and the outer layer. The fiber orientation can be improved; the regularly arranged nanofibers can be rapidly collected; axon growth is guided and promoted; the nerve repair ability is improved; nutrient transportation is facilitated; final finishing of nerve repair can be ensured; and the nerve regeneration speed can be matched with the degradation velocity.

Description

Technical field: [0001] The invention relates to the biomedical application field of polymer materials, in particular to a gradient-degradable nerve graft catheter containing oriented nanofibers and a preparation method thereof. Background technique: [0002] Denervation can lead to sensory and motor dysfunction in the innervated area, affecting the quality of life of patients to varying degrees. Autologous nerve transplantation is the most basic, safe and effective method, and the clinical recovery rate of autologous nerve transplantation can reach 80%. However, autologous transplantation is a secondary operation, with limited sources of donors, difficulty in matching tissue structure and size, long-term denervation of the donor site, and differences in transplantation efficacy, which greatly limit the widespread development of autologous nerve transplantation. [0003] The application of tissue engineering to prepare artificial nerves is an ideal choice. Artificial nerve ...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/18A61L27/24A61L27/22D04H1/74D04H1/728
Inventor 尹桂波陈和春陈桂香郭立强金春奎
Owner NANTONG TEXTILE VOCATIONAL TECH COLLEGE
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