Sesquiterpenoids in zedoary as well as preparation method and application of sesquiterpenoids
A technology of sesquiterpenoids and compounds, applied in the separation/purification of carbonyl compounds, digestive system, organic chemistry, etc., to achieve the effect of novel structure
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Embodiment 1
[0027] Example 1: Extracting and separating sesquiterpenoids from Ezhu
[0028] Curcuma longa ( Curcuma ) Perennial herbaceous plant Zezhu Curcuma phaeocaulis The dried stems of Val (10Kg) were extracted with 90% (V / V) ethanol-water heating and reflux, the extract was concentrated, and ethyl acetate and n-butanol were used to extract equal volumes for 3 times respectively. From the total sesquiterpene fraction (262 g) of Zedoary Curcuma, 100 g of the sample was subjected to silica gel column chromatography and gradient elution with cyclohexane-acetone (98:2~0:100) to obtain 9 sub-fractions (Fr.1~Fr. 9). Subfraction Fr.3 (12 g) was eluted with petroleum ether-ethyl acetate (98:2~0:100) gradient to obtain 6 subfractions (Fr.3-1~Fr.3-6). Fr.3-3 (3 g) was subjected to Sephadex LH-20 column chromatography, eluted with chloroform-methanol (1:1), and then preparative thin-layer chromatography (petroleum ether-ethyl acetate) to obtain compound 5 (144 mg) and Compound 10 (280 mg)...
Embodiment 2
[0048] Embodiment 2: Sesquiterpene 1-16 in Curcumae inhibits the release of nitric oxide (NO) from lipopolysaccharide-induced mouse mononuclear macrophage RAW 264.7
[0049] Mouse mononuclear macrophages RAW 264.7 (ATCC TIB-71) were cultured in 10% heat-inactivated (56°C, 30min) fetal bovine serum (FBS), 100 U / mL penicillin sodium (Gibco), 100 μg / mL chain Mycin (Gibco) in RPMI 1640 (Gibco) medium at 37°C, 5% CO 2 grown in a constant temperature incubator. Because NO is extremely unstable, it is quickly metabolized into nitrous acid (NO2) in the cell culture supernatant. - ), so the Griess method was used to measure NO2 in the sample - concentration as an indicator of NO levels. Griess reagent A: 0.1% N-naphthylene diamine dihydrochloride dissolved in water; Griess reagent B: 1% sulfanilamide dissolved in 5% H 3 PO 4 middle. Mix reagents A and B in equal volumes before use. RAW 264.7 cells were diluted to a concentration of 5×105 cells / mL with RPMI 1640 culture medium,...
Embodiment 3
[0051] Embodiment 3: the preparation of granule
[0052] Take 1 g of the total sesquiterpene parts of Curcuma curcuma obtained in Example 1, mix with 29 g of starch, add water to make a soft material, pass through a 12-mesh sieve for granulation, and obtain granules after drying. This granule contains 10 mg of total sesquiterpene parts per 300 mg.
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