Acellular biological patch, preparation method and apparatus thereof

A technology of biological patch and preparation device, which is applied in the field of medical biomaterials in tissue engineering, can solve the problems of incomplete decellularization and antigen removal, inflammatory reaction, immune rejection, etc., so as to overcome uneven mechanical strength and avoid immune Rejection, the effect that is conducive to industrial production

Active Publication Date: 2013-08-21
SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] To sum up, there are still technical problems in the preparation of bioscaffold materials by using SIS: decellularization and antigen removal are not thorough enough, which will lead to postoperative inflammatory reactions, pain, etc., and there is a potential risk of viral infection after implantation; freezing The drying method affects the protein activi

Method used

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  • Acellular biological patch, preparation method and apparatus thereof
  • Acellular biological patch, preparation method and apparatus thereof
  • Acellular biological patch, preparation method and apparatus thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0056] Embodiment 1. One of the method and device for preparing decellularized biological patch

[0057] The preparation method and steps of this embodiment are as follows:

[0058] Step 1. Pre-treatment: The pig small intestine, which has been dissected by removing the mucosal layer and lymph nodes, is washed with purified water until there is no dirt in the intestines, and then soaked in a 2.0% hydrochloric acid aqueous solution with a volume concentration for 2.5 hours, and then purified with Wash with water to pH 6.0~8.0.

[0059] Step 2. Disinfection treatment: Place the pig small intestine treated in Step 1 in a 15% hydrogen peroxide solution, soak for 0.5 hours, and wash with purified water for 3 times, then scrape the muscle layer and serous membrane layer with a blade to preserve the mucosa Lower layer: The submucosa of the small intestine is soaked in 70% ethanol solution for 1 hour, then rinsed with purified water until it is tasteless.

[0060] Step 3. Decellularization a...

Example Embodiment

[0066] Example 2: Preparation method and device of decellularized biological patch II

[0067] The preparation method and steps of this embodiment are as follows:

[0068] Step 1. Pre-treatment: The pig small intestine, which has been dissected by removing the mucosal layer and lymph nodes, is cleaned with purified water until there is no dirt in the intestine, and then soaked in a lactic acid aqueous solution with a volume concentration of 3.0% for 1.5 hours, and then purified Wash with water to pH 6.0~8.0.

[0069] Step 2. Disinfection treatment: Put the pig small intestine treated in Step 1 in a 1% hydrogen peroxide solution, soak for 2.5 hours, wash with purified water for 2 times, scrape off the muscle layer and serosal layer with bamboo, and keep it Submucosa: The submucosa of the small intestine is soaked in isopropanol solution for 3 hours, then rinsed with purified water until it is tasteless.

[0070] Step 3. Decellularization and de-antigen: Using a mixed solution of hyper...

Example Embodiment

[0074] Embodiment 3. The third of preparation method and device of decellularized biological patch

[0075] The preparation method and steps of this embodiment are as follows:

[0076] Step 1. Pre-treatment: The pig small intestine, which has been dissected by removing the mucosal layer and lymph nodes, is washed with purified water until there is no dirt in the intestine, and then soaked in a 1.5% volume concentration of peracetic acid solution for 1.0 hour, then Wash with purified water to pH 6.0~8.0.

[0077] Step 2. Disinfection treatment: Put the pig small intestine treated in step one in a 3% hydrogen peroxide solution, soak for 1.5 hours, wash with purified water for 3 times, scrape off the muscle layer and serous membrane layer with a stainless steel plate, and keep it Submucosa: The submucosa of the small intestine is soaked in 80% ethanol solution for 2 hours, then rinsed with purified water until it is tasteless.

[0078] Step 3. Decellularization and de-antigen: Using a m...

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Abstract

The invention relates to an acellular biological patch, a preparation method and an apparatus thereof. In the invention, small intestines of pigs are taken as raw materials, which are subjected to a series of decellularization treatment by means of an acid, hydrogen peroxide, a mixed solution of high salt and alkali so as to obtain the acellular biological patch. According to the preparation method, after treatment by the hydrogen peroxide, the mixed solution of high salt and alkali can be employed to conduct twice recycling treatment, thus reaching the effects of thorough decellularization and antigen removal. The employment of a transverse and longitudinal alternating laying way maintains uniformity of the patch's mechanical properties, the tensile strength, suture tear force, tear strength and other mechanical indexes can well meet clinical demands, thus facilitating surgical suture operation. The employed drying mode has no need of any adhesive and suture line for fixation, so that a natural three-dimensional support structure of the product is maintained, and after implantation into the human body, no strong inflammatory response and immunological rejection response can occur. The patch has no toxicity on cells, so that infection and adhesion cannot occur in a repair process. The acellular biological patch provided in the invention is suitable for repair of body tissue defects and soft tissue injuries, and also can be used in the bioengineering field to serve as the tissue for reinforcing repair of membranous defects and infected wounds and serve as a biological scaffold material, etc.

Description

technical field [0001] The invention belongs to the medical biomaterial technology of tissue engineering, in particular to a natural decellularized biological patch and its preparation method and preparation device. Background technique [0002] Extracellular matrix (ECM) is a macromolecule synthesized and secreted by animal cells, distributed on the cell surface or between cells, mainly some polysaccharides and proteins or proteoglycans, these substances form a complex network framework Structure, supports and connects tissue structures, regulates the occurrence of tissues and the physiological activities of cells. The extracellular matrix is ​​the main tissue component of various tissues and organs. It provides a suitable place for the survival and activity of cells, participates in the regulation of embryonic development, determines the adhesion and migration of cells, and plays an important role in wound repair and fibrosis, cell growth and differentiation. , metabolism...

Claims

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Application Information

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IPC IPC(8): A61L31/08A61L31/16
Inventor 张锐利刘博文刘影
Owner SHAANXI BIO REGENERATIVE MEDICINE CO LTD
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