Acellular biological patch, preparation method and apparatus thereof

Abstract

The invention relates to an acellular biological patch, a preparation method and an apparatus thereof. In the invention, small intestines of pigs are taken as raw materials, which are subjected to a series of decellularization treatment by means of an acid, hydrogen peroxide, a mixed solution of high salt and alkali so as to obtain the acellular biological patch. According to the preparation method, after treatment by the hydrogen peroxide, the mixed solution of high salt and alkali can be employed to conduct twice recycling treatment, thus reaching the effects of thorough decellularization and antigen removal. The employment of a transverse and longitudinal alternating laying way maintains uniformity of the patch's mechanical properties, the tensile strength, suture tear force, tear strength and other mechanical indexes can well meet clinical demands, thus facilitating surgical suture operation. The employed drying mode has no need of any adhesive and suture line for fixation, so that a natural three-dimensional support structure of the product is maintained, and after implantation into the human body, no strong inflammatory response and immunological rejection response can occur. The patch has no toxicity on cells, so that infection and adhesion cannot occur in a repair process. The acellular biological patch provided in the invention is suitable for repair of body tissue defects and soft tissue injuries, and also can be used in the bioengineering field to serve as the tissue for reinforcing repair of membranous defects and infected wounds and serve as a biological scaffold material, etc.

Description

technical field [0001] The invention belongs to the medical biomaterial technology of tissue engineering, in particular to a natural decellularized biological patch and its preparation method and preparation device. Background technique [0002] Extracellular matrix (ECM) is a macromolecule synthesized and secreted by animal cells, distributed on the cell surface or between cells, mainly some polysaccharides and proteins or proteoglycans, these substances form a complex network framework Structure, supports and connects tissue structures, regulates the occurrence of tissues and the physiological activities of cells. The extracellular matrix is ​​the main tissue component of various tissues and organs. It provides a suitable place for the survival and activity of cells, participates in the regulation of embryonic development, determines the adhesion and migration of cells, and plays an important role in wound repair and fibrosis, cell growth and differentiation. , metabolism...

AI Technical Summary

Problems solved by technology

[0012] To sum up, there are still technical problems in the preparation of bioscaffold materials by using SIS: decellularization and antigen removal are not thorough enough, which will lead to postoperative inflammatory reactions, pain, etc., and there is a potential risk of viral infection after implantation; freezing The drying method affects the protein activity of the product, thereby affecting the connection of the tissue structure of the material and the physiological activities of the cells; the mechanical strength of the multi-layer biomaterial is not uniform, which may easily cause the patch to tear and shift, and sutures to fix the multi-layer material are prone to foreign body reactions, immune rejection, etc.

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