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Ultrahigh cross-linked resin SX-01 and application thereof

An ultra-high cross-linking resin and resin technology, applied in the biological field, can solve the problems of high consumption, pollution, and many unit operations, and achieve the effect of reducing dosage, reducing environmental pollution, and good adsorption selectivity

Inactive Publication Date: 2013-08-21
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the industry generally adopts the anion exchange method to separate uridine acid. This process is mature, but there are many unit operations and the regeneration of the anion exchange resin needs to consume a large amount of reagents such as acids and alkalis, causing serious pollution.

Method used

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  • Ultrahigh cross-linked resin SX-01 and application thereof
  • Ultrahigh cross-linked resin SX-01 and application thereof
  • Ultrahigh cross-linked resin SX-01 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the acquisition of UMP feed liquid

[0036]RNA enzymatic hydrolysis process, using nuclease solution to act on RNA solution to obtain 4 kinds of 5'-mononucleotides, namely 5'-adenylic acid (AMP), 5'-cytidylic acid (CMP), 5'- Guanylate (GMP) and 5'-Uridylic acid (UMP). Specific steps are as follows:

[0037] (1) Prepare 600mL of 6% RNA solution with pH=5.5. First adjust the pH to 12 with NaOH, then add RNA (36g) to dissolve, then adjust the pH to 5.5 with NaOH, and place in a 70°C water bath to preheat to 70°C.

[0038] (2) Add 50 mL of enzyme solution preheated for half an hour to the RNA solution, shake well, control the reaction temperature at 70°C, and perform enzymatic hydrolysis for 185 minutes.

[0039] (3) Add 0.2% (1.2g) activated carbon and continue enzymatic hydrolysis for 50 minutes.

[0040] (4) Finally, cool to 45°C for enzymatic hydrolysis, centrifuge (400rpm, 10min), and filter to obtain RNA enzymatic hydrolysis solution.

[0041] Pass ...

Embodiment 2

[0042] Embodiment 2: the acquisition of super high cross-linked resin SX-01

[0043] In a clean and dry 2000mL three-neck flask, add 200g of dry chloromethylated polystyrene resin (chlorine balls), add 1200g of o-nitroethylbenzene, stir and mix well, fully swell at room temperature for more than 2 hours, stir and add Methylamine 4 times the weight of chlorine balls, then add 20g of anhydrous ferric chloride as a catalyst, program temperature rise to 135-150°C under stirring, and then carry out Friedel-Crafts post-crosslinking reaction at this temperature for a certain period of time to control the residual chlorine content At 1-3%, stop the reaction, suck out the mother liquor after cooling, and repeatedly extract the resin with industrial alcohol until the extraction effluent is colorless and clear, then wash the resin with 4% hydrochloric acid and distilled water until it is neutral, and dry it for later use.

[0044] The obtained product is in the shape of brown round parti...

Embodiment 3

[0045] Embodiment 3: the drawing of the adsorption isotherm of ultra-high crosslinking resin SX-01

[0046] Figure 5 The adsorption isotherm of UMP is simulated by Langmuir. The experimental process is: prepare UMP solutions with different concentrations of pH=1.6, pipette 50mL into 100mL Erlenmeyer flasks, and add 5g of ultra-high crosslinking resin SX-01 respectively. After sealing, place them in constant temperature shakers at 293K, 313K, and 333K respectively, set the rotation speed at 160rpm, and measure the concentration of UMP in the solution after adsorption equilibrium. The experimental data were fitted using the Langmuir isotherm equation.

[0047] The result is as Figure 5 It can be seen that the adsorption process of UMP conforms to the Langmuir adsorption isotherm model. With the increase of temperature, the adsorption capacity of ultra-high cross-linked resin SX-01 to UMP decreased, indicating that the adsorption process of the resin to UMP may be an exother...

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Abstract

The invention discloses ultrahigh cross-linked resin SX-01 and a preparation method thereof. The ultrahigh cross-linked resin SX-01 is prepared by sufficiently swelling chloromethylated polystyrene resin in nitroethylbenzene the weight of which is 3-8 times that of chloromethylated polystyrene resin; after swelling, adding an amination reagent the weight of which is 2-5 times that of chloromethylated beads, then adding ferric trichloride the weight of which is 10-30% that of the chloromethylated beads to be used as a Friedel catalyst, carrying out Friedel-Crafts and crosslinking reaction at the temperature of 100-150 DEG C, stopping reaction when the content of residual chlorine in the resin is 1-3%, cooling and filtering resin spheres after the reaction is finished, washing with ethanol and distilled water, and carrying out vacuum drying. The invention also discloses a new method for separating pyrimidine nucleotide by utilizing the resin. According to the new method, an adsorption column is adopted for adsorbing and separating uridylic acid, so that dosage of acid and base in an operation process can be reduced, and discharge of waste water can be reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to superhigh cross-linking resin SX-01 and its application. Background technique [0002] 5'-nucleotide is the hydrolysis product of ribonucleic acid, which is widely used in the fields of food, medicine and agriculture. For example, as a functional food additive, it is added to foods such as bread and biscuits, especially to infant milk powder, which can help infant metabolism and improve infant immunity. At the same time, uridine acid can participate in the biosynthesis of glucuronic acid, a detoxifying substance in the liver, and its disodium salt can be made into tablets and injections for medicinal products. It can also be used in the manufacture of uridine triphosphate (UTP), polyadenylation, fluterolone, arabouridine and other drugs. In addition, mixtures of nucleotides have been used in agriculture for many years as plant growth regulators. The application of nucl...

Claims

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Application Information

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IPC IPC(8): C08F112/08C08F8/32C08J3/24B01J20/26C07H19/20C07H1/06
Inventor 应汉杰刘亚南吴菁岚周精卫庄伟周建鑫
Owner NANJING UNIV OF TECH
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