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Preparation method of plant protein derivative with physiological activity and nutrition supplementing function

A technology of physiological activity and derivatives, applied in the field of preparation of vegetable protein derivatives, can solve the problem of few peptides research

Active Publication Date: 2014-09-24
SHANGHAI NONGLE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are many studies on enzymatic hydrolyzates of plant proteins, but there are few studies on obtaining peptides rich in cysteine ​​residues through a series of treatments on plant proteins. It is hoped that the present invention can fill the gap in China and obtain a Preparation method of plant protein derivative with physiological activity and nutritional supplement function

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Heat treatment of protein

[0036] Heat treatment can denature the protein and expand the spherical structure, which is conducive to hydrolysis, but heat treatment may cause protein aggregation, and may also cause thiol-disulfide bond exchange to produce other forms of substances. Differential scanning calorimetry (DSC) / non-reducing SDS-PAGE was used to analyze the degree of denaturation. Under the premise of reaching the expected degree of denaturation, find a suitable heating temperature and time to make the loss of disulfide bonds less (determination of disulfide bonds) content). The DSC results showed that the denaturation degree of the protein solution could reach more than 90% when heated at 80℃ for 20min; at 90℃ for 5min; In addition, under these heat treatment conditions, there is almost no loss of protein disulfide bond content, so the optimal temperature and time combination was selected for heating at 80 °C for 20 min as the heat treatment before p...

Embodiment 2

[0040] Example 2 Enzymatic hydrolysis method

[0041] The glycinin solution after 5% treatment, the temperature is controlled by a circulating water bath at 60 °C, the pH is controlled by a precision pH meter to 8.0 ± 0.1 (during the hydrolysis process, the pH is kept constant by adding 0.5M standard alkali dropwise), and 3% alkaline protease is hydrolyzed for 3h. ; After alkaline protease hydrolysis, continue pepsin hydrolysis, adjust pH 2.0, control pH 2.0 ± 0.1 with a precision pH meter (drip 0.5M hydrochloric acid to maintain pH constant during hydrolysis), and control temperature in a circulating water bath to 37 ℃, hydrolyzed by 2% pepsin for 2h, refrigerated and centrifuged at 5000g for 20min to obtain the supernatant, and the desired crude peptide product can be obtained. The degree of hydrolysis at the end of the hydrolysis process was about 20% monitored by pH-Stat method.

Embodiment 3

[0042] Example 3 BH reduction method of enzymatic hydrolysate

[0043] For the crude peptide product obtained in Example 2, add sodium borohydride (30% BH dissolved in 1M sodium hydroxide) to a final concentration of 1M, add 2 drops of octanol for defoaming, react at 50°C for 30min, add concentrated hydrochloric acid ( to pH2 or so) to terminate the reaction and remove excess sodium borohydride and keep the system in a lower pH state to prevent oxidation of sulfhydryl groups. The method of Example 5-4,4'-dithiodipyridine (4-DPS) method was used to quantitatively measure the sulfhydryl group to detect the reduction effect. The proportion of free sulfhydryl groups in the total sulfhydryl groups was greater than 95%.

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Abstract

The invention discloses a preparation method of a plant protein derivative with physiological activity and a nutrition supplementing function and belongs to the field of plant protein deep processing. According to the preparation method disclosed by the invention, the plant protein is used as a raw material and subjected to the processes of enzyme hydrolysis, clostridium thermoaceticum, biochemical separation and the like, and the product is a plant protein enzymatic hydrolysate with cysteine residue peptide as the important ingredient. According to the preparation method disclosed by the invention, abundant plant protein resource containing cysteine is used and subjected to enzymatic hydrolysis to obtain the plant protein derivative with the physiological activity and the nutrition supplementing function, and therefore the preparation method is of great significance.

Description

technical field [0001] The invention relates to a preparation method of a plant protein derivative with physiological activity and nutritional supplement function, and belongs to the field of deep processing of plant protein. Background technique [0002] Cysteine ​​is an aliphatic thiol-containing polar α-amino acid that can be converted from methionine in the body. Cysteine ​​has many physiological functions: it has a chelating effect in organisms, so it has a wide range of poisons such as formaldehyde, acetaldehyde, chloroform, carbon tetrachloride, lead, cadmium, methylmercury chloride, lipid peroxide, PCB , fugu poison, alcohol, etc. have effective detoxification effects, which have been proved by experiments; maintain the activity of important sulfhydryl enzymes in the keratin production of skin proteins, and supplement sulfhydryl groups to maintain normal metabolism of the skin and regulate the epidermis. The underlying melanin produced by the pigment cells in the lo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/16C07K1/18C07K1/22
Inventor 华欲飞孔祥珍王金艳丁秀臻张彩猛
Owner SHANGHAI NONGLE BIOLOGICAL PROD
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