Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of modified PLGA-PEG nano-fiber blanket

A nanofiber, modification technology, applied in the field of preparation of modified PLGA-PEG nanofiber mat, to achieve the effect of easy product, wide application prospect and simple process

Inactive Publication Date: 2013-08-28
DONGHUA UNIV
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Up to now, there is no literature report on the preparation of PLGA-PEG nanofiber mat by electrospinning method, and use the nanofiber mat as a drug carrier, and use the hydrophilic drug amoxicillin as a model drug to further evaluate the nanofiber as a drug carrier. Research Report on Sustained-release Performance, Biological Activity and Blood Compatibility of Carriers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of modified PLGA-PEG nano-fiber blanket
  • Preparation method of modified PLGA-PEG nano-fiber blanket
  • Preparation method of modified PLGA-PEG nano-fiber blanket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Take 1.5g of PLGA, mix it with 6ml of chloroform, and stir at room temperature for 6h until PLGA is completely dissolved. Then add 45mg of EDC to activate the carboxyl group at the end of PLGA, the activation time is 3-4 hours, then add 230mg of mPEG-NH 2 , stirred at room temperature for 3 days. The reaction solution was slowly added dropwise into iced methanol, the unreacted mPEG-NH 2 Soluble in methanol, PLGA-PEG precipitates. The solid was taken out and washed three times with ice methanol again. The obtained product was dried in a vacuum oven for 3 days. Take out 5mg of PLGA-PEG, dissolve it with deuterated chloroform and transfer it to an NMR tube. The product is evaluated by nuclear magnetic resonance (NMR), and the NMR test results (see attached figure 1 ) showed that the three characteristic peaks of PLGA appeared at 1.6ppm, 4.8ppm and 5.2ppm respectively; while the characteristic peaks of PEG appeared at 3.7ppm. Therefore, it can be confirmed that the ob...

Embodiment 2

[0047] Weigh 0.5g of PLGA and PLGA-PEG into a vial, add 1.5ml of THF and 0.5ml of DMF, stir at room temperature until completely dissolved, add 5mg of AMX to the two vials respectively, and make AMX and PLGA and PLGA -The mass ratio of PEG reaches 1:100, and ultrasonic stirring makes AMX uniformly dispersed, so as to obtain a uniform electrospinning solution.

[0048] AMX / PLGA and AMX / PLGA-PEG drug-loaded nanofiber mats were then prepared by conventional electrospinning. Among them, the receiving distance is 15cm, the voltage is 18kV, and the flow rate is 0.8mL / h. The prepared nanofiber mat is dried in a vacuum oven for 48h to remove residual moisture and solvent. SEM observation results show that the obtained AMX / PLGA and AMX / PLGA-PEG nanofibers have regular morphology and regular surface, and the fiber diameters are 891±195nm and 847±206nm respectively (see attached figure 2 ). Comparing the diameters of these two different fibers, it can be found that under the same spin...

Embodiment 3

[0050] 100 mg of the electrospun AMX / PLGA and AMX / PLGA-PEG nanofiber mats obtained in Example 2 were respectively placed in a reagent bottle containing 10 mL of PBS buffer for sustained release experiments.

[0051] Place the reagent bottle in a shaker at 37°C and shake it. At different time points, 1.5 mL of the solution is taken out of the reagent bottle, and then supplemented with 1.5 mL of PBS buffer. The concentration of the taken-out sustained-release solution was tested with a UV spectrophotometer, and the concentration-absorbance calibration curve of AMX in PBS was used to calculate the sustained-release percentage at different time points and analyze the drug release kinetics of the PLGA and PLGA-PEG carrier drug-loaded systems. . From attached image 3 It can be seen that compared with the AMX / PLGA nanofiber drug-carrier system, the electrospun AMX / PLGA-PEG nanofiber carrier drug-loaded system can effectively slow down the "burst release" phenomenon of the drug, and...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of a modified PLGA-PEG nano-fiber blanket. The preparation method of the modified PLGA-PEG nano-fiber blanket comprises the steps of dissolving PLGA in solvent, adding EDC to the solvent, adding mPEG-NH2 to the solvent after reaction of 3-5 hours, crystallizing, purifying and drying after reaction of 3-5 days, obtaining the PLGA-PEG, then adding the PLGA-PEG to the solvent, stirring, obtaining a spinning solution, adding AMX to the spinning solution, stirring, spinning in an electrostatic mode, drying in a vacuum mode, and then obtaining the modified PLGA-PEG nano-fiber blanket. The preparation method is simple in process, and products are easy to obtain. The PLGA and mPEG-NH2 used in the preparation method are low in cost and have good biocompatibility. The prepared PLGA-PEG nano-fiber blanket has good blood compatibility and biocompatibility, slows down the phenomenon of burst release at the same time, is an excellent drug carrier, and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of nanofiber preparation, in particular to a preparation method of modified PLGA-PEG nanofiber felt. Background technique [0002] In recent years, with the vigorous rise of nanotechnology and the continuous emergence of various biocompatible polymer materials, nanoscale drug delivery systems based on polymer materials have attracted widespread attention. In order to change the burst release phenomenon of traditional medicines, improve the utilization rate of medicines and reduce the toxic and side effects on human body, the development of drug controlled release system with sustained release effect has always been a research hotspot. [0003] Electrospinning technology is a method in which a charged polymer solution (or melt) flows and deforms in an electrostatic field, and is solidified by volatilization of the solvent or cooling of the melt to obtain a fibrous substance. The biodegradable and biocompatible polymer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/4326D04H1/728C08G81/00D01F6/96D01F1/10
Inventor 史向阳张乐强
Owner DONGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com