Bacterial cellulose three-dimensional microporous scaffold preparation method

A bacterial cellulose and microporous technology, applied in medical science, prosthesis, etc., can solve the problems of poor controllability of porosity and pore distribution, random pore size variation, low degree of inter-penetration of pores, etc., to achieve good structural stability, Good mechanical strength, quick preparation effect

Active Publication Date: 2013-09-04
钟春燕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can obtain successful tissue engineering scaffolds, the obtained tissue engineering scaffolds lack mechanical strength, low degree of interpenetration of pores, poor con

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The bacterial cellulose obtained from the fermentation culture of Acetobacter xylinum was soaked in 1% NaOH aqueous solution by weight, heated at 100°C for 3 hours, and then washed repeatedly with twice distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane. Freeze-dry at -10°C to obtain bacterial cellulose scaffolds.

[0017] At 10°C, a carbon dioxide laser machine was used to perform laser drilling on bacterial cellulose scaffolds. The micropore processing is carried out along the directions of three-dimensional coordinate axes (X axis, Y axis and Z axis) of the bacterial cellulose scaffold respectively. The processed bacterial cellulose scaffolds were washed with double distilled water. Under the condition of -40°C, freeze-dry to obtain the bacterial cellulose three-dimensional microporous scaffold. The micropore diameter is 100 μm, and the micropore spacing is 0.8 mm.

Embodiment 2

[0019] The bacterial cellulose obtained from the fermentation culture of Rhizobium was soaked in 3% NaOH aqueous solution by weight, heated at 80°C for 4 hours, and then washed repeatedly with double distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane. Freeze-dry at -20°C to obtain bacterial cellulose scaffolds.

[0020] At 8°C, a carbon dioxide laser machine was used to perform laser drilling on bacterial cellulose scaffolds. The micropore processing is carried out along the directions of three-dimensional coordinate axes (X axis, Y axis and Z axis) of the bacterial cellulose scaffold respectively. The processed bacterial cellulose scaffolds were washed with double distilled water. Under the condition of -30°C, freeze-dry to obtain the bacterial cellulose three-dimensional microporous scaffold. The micropore diameter is 150 μm, and the micropore spacing is 1.0 mm.

Embodiment 3

[0022] The bacterial cellulose obtained from the fermentation culture of Sarcina was soaked in 5% NaOH aqueous solution by weight, heated at 60°C for 5 hours, and then washed repeatedly with twice distilled water until neutral. Remove bacterial protein and residual culture medium adhering to the cellulose membrane. Freeze-dry at -30°C to obtain bacterial cellulose scaffolds.

[0023] At 4°C, an excimer laser was used to perform laser drilling on bacterial cellulose scaffolds. The micropore processing is carried out along the directions of three-dimensional coordinate axes (X axis, Y axis and Z axis) of the bacterial cellulose scaffold respectively. The processed bacterial cellulose scaffolds were washed with double distilled water. Under the condition of -20°C, freeze-dry to obtain the bacterial cellulose three-dimensional microporous scaffold. The micropore diameter is 250 μm (100, 150, ), and the micropore spacing is 1.5 mm.

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Abstract

The invention discloses a bacterial cellulose three-dimensional microporous scaffold preparation method which relates to the technical field of scaffold material preparation processing. The preparation method provided by the invention comprises the following steps: carrying out purification processing on bacterial cellulose generated by bacterial strain fermentation and carrying out freeze drying to obtain bacterial cellulose scaffold; and processing the bacterial cellulose scaffold in the environment of minus 5-10 DEG C by the adoption of a laser drilling technology, washing the processed bacterial cellulose scaffold by the use of redistilled water, and carrying out freeze drying to obtain the bacterial cellulose three-dimensional microporous scaffold, wherein the diameter of the micropores is 100-500 microns, and micropore spacing is 0.8-2.5mm. The preparation method provided by the invention requires a simple technology and is convenient to operate. Micropore size and three-dimensional micropore structure of the scaffold can be regulated and controlled by means of controlling technological parameters and the like. The prepared bacterial cellulose three-dimensional microporous scaffold can be applied in the field of tissue engineering.

Description

technical field [0001] The invention relates to the technical field of preparation and processing of biological scaffold materials. In particular, it relates to a preparation method of bacterial cellulose three-dimensional microporous support. Background technique [0002] Tissue engineering refers to the application of the principles and technologies of life science and engineering to design, build, maintain the growth of human cells and tissues, and restore the function of damaged tissues or organs. The core is to culture and expand normal tissue cells in vitro. , adsorbed on a porous three-dimensional biomaterial scaffold with good biocompatibility and can be absorbed by the body to form an active complex, and then the cell-biomaterial composite is implanted into the lesion of the body tissue and organ, and the cells are gradually absorbed by the biomaterial. During the process of degradation and absorption, the body forms new substitutes that are consistent with the dam...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/56
Inventor 钟春燕其他发明人请求不公开姓名
Owner 钟春燕
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