Feeding microecological additive and compound feed containing same

An additive and micro-ecological technology, applied in animal feed, animal feed, application, etc., can solve problems such as human injury, achieve the effect of increasing daily weight gain, reducing feed-to-egg ratio and diarrhea rate, and improving feed conversion rate

Inactive Publication Date: 2013-10-09
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, in the breeding industry, there is still a large-scale use of additives such as antibiotics and hormones. Although these substances increase the total amount of livestock and poultry products, their residues in food will cause harm to the human body

Method used

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  • Feeding microecological additive and compound feed containing same
  • Feeding microecological additive and compound feed containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The mensuration of embodiment 1 bacillus licheniformis CGMCC No.7030 fermented liquid enzyme activity

[0019] Inoculate the test bacteria on beef extract peptone solid medium, culture at 30°C for 24 hours, pick the strains, suspend in 0.85% normal saline, adjust the concentration to about 5.0×10 7 Inject 2mL of bacterial suspension into a 100mL Erlenmeyer flask containing 20mL of enzyme-producing medium, naturally supply oxygen at 30°C, ferment and cultivate at 170r / min for 24h, and centrifuge the liquid fermentation broth at 5000r / min for 15min , take the supernatant, which is the crude enzyme solution.

[0020] The formula of cellulase production medium is: peptone 0.9%, yeast extract 1.0%, CMC-Na 0.5%, pH8.0.

[0021] The formula of protease production medium is: peptone 1.0%, yeast extract 0.5%, glucose 1.0%, soluble starch 0.5%, KH 2 PO40.2%, MgSO 4 "7H 2 O0.05%, CaCl 2 "2H 2 O0.02%, pH7.2.

[0022] (1) Determination of cellulase activity

[0023] Determin...

Embodiment 2

[0031] Example 2 Preparation of Bacillus licheniformis CGMCC No.7030 freeze-dried powder

[0032] The preparation method of bacillus licheniformis bacteria powder, comprises the steps

[0033] (1) Plate culture rejuvenation: inoculate the Bacillus licheniformis strain on the plate medium, culture at 30°C for 18 hours, rejuvenate the Bacillus licheniformis and form a single colony, pick a single colony on the plate medium for inoculation, 30°C Cultivate for 24 hours;

[0034] The formula of the plate medium is: tryptone 1%, yeast extract 0.5%, sodium chloride 1%, agar 2%, pH 7.0±0.2.

[0035] (2) Preparation of primary seed solution: Transfer the Bacillus licheniformis cultured in step (1) to a test tube containing 10 mL of medium, and culture it on a shaker at 30°C for 16 hours at a speed of 170 rpm, so that it is in the late logarithmic period. , to obtain the first-grade seed solution;

[0036] (3) Preparation of the secondary seed solution: Inoculate the primary seed sol...

Embodiment 3

[0041] The preparation of embodiment 3 Bacillus subtilis freeze-dried powder

[0042] 1. Preparation of Bacillus subtilis CGMCC No.6511 bacterial powder

[0043] 1) Preparation of Bacillus subtilis seed solution: Inoculate the strains preserved on the slant of the test tube into a test tube containing 15 mL of culture medium, and cultivate in a constant temperature shaker at 37°C for 16-20 hours at a speed of 225 rpm until the number of viable bacteria reaches 10 7 CFU / mL, inoculate at 1% in a 250mL Erlenmeyer flask containing 100mL seed medium, culture in a constant temperature shaker at 37°C for 16-20h, the shaker speed is 225rpm, until the number of viable bacteria reaches 10 7 CFU / mL, used as seed solution for later use.

[0044] The seed medium is: glucose, 20.0g / L; peptone, 10.0g / L; yeast powder, 4.0g / L; sodium chloride, 5g / L; distilled water is added to 1000mL, pH7.0±0.2.

[0045] 2) Liquid submerged fermentation culture

[0046] A 5L vertical fermenter was selected ...

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Abstract

The invention discloses a feeding microecological additive and applications thereof, specifically to a composite microecological preparation containing a plurality of probiotics and prebiotics, and applications of the preparation. The preparation is prepared by mixing bacillus licheniformis CGMCC No. 7030, bacillus subtilis, enterococcus faecalis, saccharicterpenin and a carrier according to a certain ratio. The protrude advantages comprises: under the combined action of bacillus licheniformi, the bacillus subtilis powder, a lactobacillus powder and saccharicterpenin, intestinal structure and flora of animals are improved; the microecological additive can help to improve forage utilization rate, promote animal growth, improve output, raise immunity and partially substitute antibiotic, and thus animal product quality is improved; and the microecological additive is beneficial for animal and human health.

Description

technical field [0001] The invention belongs to the field of animal feed, and in particular relates to a feed additive prepared by mixing bacillus licheniformis, bacillus subtilis, enterococcus faecalis, saccharicterpenes and a carrier and a batch material containing the same. Background technique [0002] With the sustainable development strategy becoming more and more popular, scientific and technological progress, economic development and improvement of people's living standards, in recent years, pollution-free food and green food have become people's consumption pursuit. However, there is still a large-scale use of additives such as antibiotics and hormones in the breeding industry. Although these substances increase the total amount of livestock and poultry products, their residues in food will cause harm to the human body. [0003] Synbiotics are probiotics and prebiotics used in combination with microecological preparations, which are characterized by the ability to p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/16A23K1/18
Inventor 李晓清王凡刘婷王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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