Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of Royal Jelly Fermented by Lactic Acid Bacteria in the Preparation of Innate Immunity Activator

A technology of lactic acid bacteria fermentation and natural immunity, applied in the direction of bacteria, application and fermentation used in food preparation, can solve problems such as the actual situation is unknown, and achieve the effect of excellent natural immunity promotion effect.

Active Publication Date: 2015-09-23
MORIKAWA KENKODO
View PDF15 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it is known that Pope Pio XII, who was aging and in a critical condition in 1954, had an amazing recovery effect due to the royal jelly prescribed by physician Gallia Seril, but its efficacy is scientifically The actual situation of the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Royal Jelly Fermented by Lactic Acid Bacteria in the Preparation of Innate Immunity Activator
  • Application of Royal Jelly Fermented by Lactic Acid Bacteria in the Preparation of Innate Immunity Activator
  • Application of Royal Jelly Fermented by Lactic Acid Bacteria in the Preparation of Innate Immunity Activator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119]

[0120] In a 300mL Erlenmeyer flask with a baffle, put 100mL of 1% glucose, 1% yeast extract liquid medium (pH 7.2) and 1mL of 10% manganese yeast liquid, cover with a gas-permeable A silicone cap (SILICOSEN (registered trademark)) was autoclaved at 121° C. for 15 minutes to prepare a liquid medium.

[0121] 50 μL of glycerol-preserved NBRC12005 strain was added to the liquid medium, and static culture was carried out at 30° C. for one day. Thereafter, 3 mL of the culture solution of the NBRC12005 strain was added to the liquid medium prepared in the same manner as above, and static culture was performed at 30° C. for 1 day to perform pre-culture of the NBRC12005 strain.

[0122]

[0123] Into a 5L-capacity mini-fermenter made by MBS Corporation, put 3L of liquid medium (pH7.2) containing 1% glucose, 1% yeast extract, and 1mL of 10% manganese yeast liquid, and perform hot pressing at 121°C for 20 minutes instrument sterilization. 75 mL of a 40% sodium glutamate m...

reference example 1

[0144] Yeast β-glucan manufactured by Oriental Yeast Co., Ltd. was used, and wakame sporophyll fucoidan was manufactured by Riken Vitamin Co., Ltd., and the innate immunity promoting effect was measured in the same manner as in Example 1 and Comparative Example 1. The results are shown in Table 1, figure 1 shown.

Embodiment 2

[0153] [Isolation of active ingredient of innate immunity activator]

[0154] (1) Preparation of hot water extract

[0155] 50 mL of 0.9% NaCl aqueous solution was added to 10 g of powder of the "innate immunity activator derived from royal jelly" obtained in Example 1, and a 200 mg / mL suspension was prepared. Then, it heated at 121 degreeC for 20 minutes using the autoclave. After cooling the suspension, 48 mL of supernatant (hot water extract fraction) was obtained by centrifugation at room temperature at 8000 rpm for 5 minutes using a high-speed centrifuge (Tianli Koki CR-21, rotor R10A2). Thereafter, the centrifuged supernatant was dispensed by 16 mL, 99.5% ethanol was added by 32 mL each, stirred well, and centrifuged at room temperature at 8000 rpm for 5 minutes to obtain 1.5 g (dry weight) of a white precipitate (ethanol precipitated fraction). 400 mg of the precipitate was dissolved in 8 mL of 10 mM Tris-HCl buffer (pH 7.9), and the supernatant was collected by cent...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention addresses the problem of providing: a novel "method for producing a royal jelly-derived innate immunity activator having enhanced innate immunity promoting activity", which has excellent innate immunity promoting activity; and a royal jelly-derived innate immunity activator which is produced by the production method. The problem is solved by a method for producing an innate immunity activator having enhanced innate immunity promoting activity, said method being characterized by comprising at least a step wherein royal jelly is fermented using lactic acid bacteria. In addition, the problem is solved by a method for producing an innate immunity activator having enhanced innate immunity promoting activity, said method comprising at least the following steps (1) to (3): (1) a step wherein lactic acid bacteria are precultured; (2) a step wherein royal jelly is fermented using the lactic acid bacteria; and (3) a step wherein sterilization of the lactic acid bacteria is carried out.

Description

technical field [0001] The invention relates to the application of royal jelly fermented by lactic acid bacteria in preparation as natural immune activator. Background technique [0002] Royal jelly (Royal jelly; RJ) is a milky white substance mixed with the secretions of the young worker bee's pharyngeal gland and mandibular gland, and is mainly composed of water (content 66% by mass), royal jelly The unique protein (content 11 to 12% by mass; avidin (MJRP1) with cell proliferation promotion or cell death suppression (Patent Document 1), royal jelly main protein 3 with immunosuppression ( MRJP3) (non-patent document 1), royal jelly antibacterial protein (Royalisin) with antibacterial effect, sugar (content 10 mass %; fructose, glucose, sucrose, etc.), and lipids (content 5 to 7 mass %; 10-hydroxy-2-decenoic acid with bactericidal action, insulin action, anti-cancer action, and nerve cell proliferation action (Non-Patent Document 2), 3,10-hydroxydecanoic acid with immune co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/00A61K35/744A61K35/644A61K31/715A61P37/04A61K35/64A61K35/74A61K35/747
CPCA23Y2220/13A23L1/076A61K35/747A23L1/3014A61K35/644A23L21/20A23L33/135A61P37/04A23V2400/121
Inventor 片冈启子
Owner MORIKAWA KENKODO
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com