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Comprehensive technology and equipment of enzymatic hydrolysis of corn to produce starch

A corn and deep processing technology, applied in the field of enzyme fermentation, can solve the problems of reducing production, waste of soaking tanks, large amount of protease and cellulase, etc., and achieve the effect of reducing emissions and benefiting the environment

Active Publication Date: 2016-09-07
NINGXIA EPPEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, this method also has the following disadvantages: soaking requires the use of a pressurized container, which is feasible for the laboratory test carried out on a 10L scale in this patent application, but now the normal pressure soaking tanks in the factory production are frequently measured in tons, It is almost impossible to transform into a pressurized container, and all of them need to be replaced, which is expensive and causes waste of the original soaking tank; the operation and maintenance costs required for pressurization are relatively high, and there is one more accident hazard after pressurization than normal pressure operation. That is, as the equipment ages, pressurization will cause the explosion of the pressure vessel, which is not easy to promote today when safety production is increasingly valued; the amount of protease and cellulase used in it is relatively large, and the cost is relatively high. This is a long-term operating cost; protease is used in it, causing the protein to be degraded into water-soluble polypeptides and amino acids and lost, which greatly reduces the subsequent corn protein production and greatly reduces the production of a higher-margin product
In addition, although the soaking time of this method is short, because the current domestic corn starch processing industry is generally located in relatively remote areas, the site is sufficient, and the normal pressure soaking tanks are cheap and easy to maintain. At present, a large number of soaking tanks are generally run in parallel to overcome the long soaking time. Shortcomings

Method used

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  • Comprehensive technology and equipment of enzymatic hydrolysis of corn to produce starch
  • Comprehensive technology and equipment of enzymatic hydrolysis of corn to produce starch
  • Comprehensive technology and equipment of enzymatic hydrolysis of corn to produce starch

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1 Cloning and purification of acid-resistant cellulase

[0046] According to a strain of acid-resistant Penicillium purporogenum obtained through long-term screening by the inventor, the Institute of Microbiology of the Chinese Academy of Sciences was commissioned to clone a cellulase gene with a polynucleotide sequence as shown in SEQ ID NO: 1 through commercial channels. The cellulase encoding amino acid sequence as shown in SEQ ID NO: 2 was cloned into the yeast secretion expression vector pPICZαA (available from Invitrogen) according to the normal reading frame according to conventional methods and transformed into Pichia pastoris GS115 strain (available from From Invitrogen Company), after the PCR and sequencing checks were correct, the yeast clones with positive expression were returned to the inventor.

[0047] According to Invitrogen's manufacturer's instructions, yeast clones (single colonies) were inoculated in 25 mL of BMGH medium, and cultured at 30°...

Embodiment 2

[0052] Example 2 Enzymolysis corn deep processing process

[0053] As a comparative example, such as figure 1As shown, the corn deep processing process and equipment that have been promoted and used in China (see "Corn Starch Industry Handbook") are used for continuous production. Soak in medium and normal pressure for 72 hours; the drying step adopts the heating and drying step, so that the tail gas discharged from the dryer that performs each drying step is kept at 75~95°C, and the tail gas discharged from each drying step is collected for detection, and the results are as follows:

[0054] The sulfur dioxide content in the tail gas of the drying step in the germ treatment process is 550mg / m³ + 10%;

[0055] The sulfur dioxide content in the tail gas of the drying step in the fiber processing process is 430mg / m³ + 15%;

[0056] The sulfur dioxide content in the tail gas during the drying step of protein powder processing is 730mg / m³ + 10%; and

[0057] The sulfur dioxi...

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Abstract

The invention belongs to the technical field of enzyme fermentation and provides a method for corn enzymolysis deep-processing. The method comprises that acid-resistant cellulase is added into a sulfurous acid aqueous solution and tail gas evaporated by a drying step is subjected to desulphurization. The invention also provides a method for producing corn starch by enzymolysis based on the above method, the acid-resistant cellulase and a desulphurization treatment system.

Description

technical field [0001] The invention belongs to the technical field of enzyme fermentation, in particular, the invention relates to a comprehensive technological process for enzymatically hydrolyzing corn to produce starch and equipment used therein. Background technique [0002] Corn is one of the main food crops in the world, and my country's corn output is about 100 million tons. The most valuable part of corn is the kernel. The surface of the grain is covered with cortex, and under the cortex are germ and endosperm, among which the germ has high oil content and strong toughness; while the outer layer of endosperm is wrapped with aleurone layer (slag skin), and the inside is full of starch granules. Corn kernels are rich in nutrients, including protein, oil, cellulose, soluble sugar, minerals, etc. in addition to starch. [0003] In order to effectively utilize the various nutrients in corn, most of the current domestic corn starch processing uses such figure 1 In the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B30/04C12N9/42C12N15/56C12N15/81C12N1/19
Inventor 马吉银刘鸿曹洪刘鑫
Owner NINGXIA EPPEN BIOTECH