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Method and device for rapidly and quantitatively detecting tumor marker with immunochromatography test strip marked by quantum dots

A technology of tumor markers and immunochromatography, which is applied in the field of rapid quantitative detection of tumor markers and devices using quantum dot-labeled immunochromatography test strips, which can solve the problem that quantitative detection cannot be completed, and the color of the detection strip is light and cannot be detected by naked eyes. Identification and other issues

Inactive Publication Date: 2013-10-23
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Colloidal gold is often used as a marker in commercialized immunochromatographic test strips. Although the test results are visible to the naked eye, colloidal gold can only be used as a marker for qualitative or semi-quantitative detection, and quantitative detection cannot be completed; while some antigens Or samples with low antibody concentration have too light detection bands to be recognized by naked eyes

Method used

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  • Method and device for rapidly and quantitatively detecting tumor marker with immunochromatography test strip marked by quantum dots
  • Method and device for rapidly and quantitatively detecting tumor marker with immunochromatography test strip marked by quantum dots
  • Method and device for rapidly and quantitatively detecting tumor marker with immunochromatography test strip marked by quantum dots

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Embodiment 1

[0052] Preparation and assembly process of quantum dot-labeled immunochromatographic test strips. The quantum dots used are CdZnSe quantum dots, and the emission wavelength is 530nm; the tumor marker antibody used is β-hCG monoclonal antibody, and the ratio of quantum dots to antibodies is 30:2; the chemical coupling agent used is ethyl-( 3-Dimethylpropyl) carbodiimide hydrochloride (EDC), the ratio of quantum dots to EDC is 12:1.

[0053] 1. Antibodies related to tumor markers coupled with quantum dots

[0054] (1) Weigh 0.5mg EDC, dissolve it in 1mL deionized water, and set aside; take 6mg quantum dots and disperse them in 1mL PBS buffer, set aside; take 0.4mgβ-hCG monoclonal antibody into the quantum dot solution, vortex Rotate at a low speed on the instrument to mix evenly, add EDC aqueous solution to the quantum dot solution during the vortex process, place the above mixed solution on a rotating mixing rack, and react at room temperature for 2 hours.

[0055] (2) Purify...

Embodiment 2

[0070] Preparation and assembly process of quantum dot-labeled immunochromatographic test strips. The quantum dots used are CdTe / ZnSe quantum dots, the emission wavelength is 634nm, the tumor marker antibody used is CEA monoclonal antibody, the ratio of quantum dots to antibodies is 9:2, and the chemical coupling agent used is ethyl-( 3-Dimethylpropyl) carbodiimide hydrochloride (EDC), the ratio of quantum dots to EDC is 16:3.

[0071] 1. Antibodies related to tumor markers coupled with quantum dots

[0072] (1) Weigh 0.9mg EDC, dissolve it in 1mL deionized water, and set aside; take 9mg quantum dots and disperse them in 1mL PBS buffer, set aside; take 2mg CEA monoclonal antibody and add them to the quantum dot solution, and place on a vortex instrument Rotate at a low speed to make it evenly mixed, and add EDC aqueous solution to the quantum dot solution during the vortex process. The above mixed solution was placed on a rotating mixing rack and reacted at room temperature ...

Embodiment 3

[0088] Preparation and assembly process of quantum dot-labeled immunochromatographic test strips. The quantum dots used are ZnCuInS 3 Quantum dots, the emission wavelength is 720nm; the tumor marker antibody used is AFP monoclonal antibody, the ratio of quantum dots to antibody mass fraction is 4:1; the chemical coupling agent used is ethyl-(3-dimethylpropyl) Carbodiimide hydrochloric acid (EDC), the mass fraction ratio of quantum dots to EDC is 6:1.

[0089] 1. Antibodies related to tumor markers coupled with quantum dots

[0090] (1) Weigh 2 mg of EDC, dissolve in 1 mL of deionized water, and set aside; take 12 mg of quantum dots and disperse them in 1 mL of PBS buffer, and set aside; take 3 mg of AFP monoclonal antibody into the quantum dot solution, and rotate at a low speed on a vortex instrument Make it mix well, and add EDC aqueous solution to the quantum dot solution during the vortex process. The above mixed solution was placed on a rotating mixing rack and reacted...

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Abstract

The invention relates to a method and a device for rapidly and quantitatively detecting a tumor marker with an immunochromatography test strip marked by quantum dots. The method comprises the following steps of: replacing collaurum particles by using quantum dots as a signal marker, coupling with an antibody corresponding to the tumor marker to be tested, and spraying or directly coating on a conjugate releasing pad, wherein the antibody on another site corresponding to the tumor marker and a second antibody are coated on a nitrocellulose membrane to form a T line and a C line respectively; assembling a sample pad, a combining pad, a reaction membrane and a water absorbing pad according to a certain sequence to prepare the immunochromatography test strip; detecting qualitatively and quantitatively according to fluorescent stripes presented on the T line and the C line and the strength of fluorescence; and providing a quantum dot fluorescence immune detection device. The detection device is simple, the operation is simple and rapid, the time consumption for detection is short, and the result judgment is easy. The method and device are particularly suitable for early-stage screening, diagnosis, judgment prediction and prognosis of the tumor markers in households, communities, hospitals and the like, and evaluation of treatment effect and followed observation of high risk groups.

Description

technical field [0001] The invention relates to a method and a device for rapidly and quantitatively detecting tumor markers with immunochromatographic test strips marked with quantum dots, and belongs to the technical field of detection. Background technique [0002] Immunochromatography is a new type of detection technology that combines immunolabeling technology with chromatography technology. It uses the chromogenic properties of markers to perform qualitative, semi-quantitative or quantitative detection of analytes. It uses the microporous filter membrane as the solid phase carrier, the liquid to be tested as the mobile phase, and the liquid to be tested flows forward through the capillary action and siphon effect of the microporous filter membrane, and at the same time, the relevant components in the liquid to be tested are fixed on the membrane. Specific immune reaction occurs to the antigen or antibody on the test strip, so that the immune complex stays on the detect...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N21/64
Inventor 常津李雪杨秋花刘俊庆
Owner TIANJIN UNIV
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