Novel application of centella extract

A Centella asiatica extract and the technology of the extract are applied in the field of medicine, can solve the problems of large toxic and side effects, and achieve the effect of high activity of the extract

Inactive Publication Date: 2013-10-30
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the weight-loss drugs with definite clinical efficacy are chemically synthesized drugs, such as norepinephrine uptake inhibitors, etc., which have serious side effects after long-term use.

Method used

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  • Novel application of centella extract
  • Novel application of centella extract
  • Novel application of centella extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the preparation of fatty acid synthase inhibitor Centella asiatica effective substance

[0022] Crush Centella asiatica, and extract the effective substance by the following method.

[0023] Use ethanol-water mixed solvent (the volume percentage of ethanol is 50%) and the above-mentioned pulverized Centella asiatica by weight ratio 8: 1, stir and leach for 12 hours at room temperature at 25 ° C, filter with ordinary filter paper; Then soak for another 12 hours, then filter with ordinary filter paper, and combine the two filtrates. At 0.09MPa, 40°C (reduced pressure) low-temperature evaporation to remove ethanol to obtain an aqueous phase containing effective substances, use an equal volume of petroleum ether to remove lipids, then extract three times with an equal volume of ethyl acetate, and combine acetic acid The ethyl ester layer was evaporated under reduced pressure and low temperature to remove the organic solvent to obtain an extract, which was dri...

Embodiment 2

[0025] Embodiment 2, the content determination of Centella asiatica active extract

[0026] Crush Centella asiatica, and extract the effective substance by the following method.

[0027] Accurately weigh 1.0 g of Centella asiatica extract in Example 1, place in a 100 ml volumetric flask, add water to dissolve, and set the volume to 100 ml as the test solution. Precisely measure 5 μL, and use acetonitrile (A)-0.3% acetic acid aqueous solution (B) gradient elution: 0-4min is (A) 20%, (B) 80%; 4-20min gradient gradually becomes (A) 60%, (B) 40%; 20-30min gradient gradually becomes (A) 70%, (B) 30%; Detection wavelength is 254nm; Flow velocity is 1mL / min; Column temperature is room temperature; Waters Symmetry C18 chromatographic column (4.6mm * 250mm , 5μm), the results showed that the content was 30%.

Embodiment 3

[0028] Embodiment 3, the detection of fatty acid synthase inhibitory activity

[0029] At 37°C, add the enzyme substrate to a 2mL quartz cuvette: add 1.85mL of 0.1M pH=7.0 phosphate buffer, 25μL of 0.2mM acetyl-CoA solution, 0.4mM malonyl-CoA solution 50μL and 50μL of 1.3mM NADPH solution, then add 30μL of FAS solution and mix well to start the enzymatic reaction. The spectrophotometer monitors the change of the absorbance value A at the wavelength of 340nm per unit time, and expresses the activity of FAS by (ΔA / min). Centella asiatica medicinal materials were crushed, passed through a 40-mesh sieve, added solvent (1:20), stirred at room temperature for 4 hours, centrifuged to separate the supernatant, and diluted ten times to determine the inhibitory rate of the extract to FAS. In the presence of a certain concentration of Centella asiatica extract, the activity of FAS holoenzyme reaction decreases, and when its activity decreases by half, the concentration of Centella asiat...

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Abstract

The invention provides a method for obtaining an extract from centella. The method comprises the following steps of: carrying out soakage extraction, ultrasonic extraction or heating reflux extraction on the centella at room temperature by using ethanol as an extracting agent, wherein the extraction times can be one or more; obtaining a crude extract after drying at reduced pressure and weighing the crude extract; dispersing the crude extract in proper water; removing oil-soluble impurities from the dispersed crude extract by extraction using equal amount of any organic solvent from petroleum ether, chloroform and the like; obtaining the normal butanol extract of the centella by extraction using equal amount of normal butanol. The bioactivity of the centella extract is studied by means of in-vitro detection of the anti-fatty acid synthetase activity, and assay proves that the centella extract is strong in anti-fatty acid synthetase activity, can function as a fatty acid synthetas inhibitor, and therefore can be applied to preparing various medicine forms or dietary supplements for treating and preventing obesity in combination with proper excipients.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an extract or composition of Centella asiatica as an inhibitor of fatty acid synthase and its application in the preparation of drugs for preventing or treating obesity and weight loss products. Background technique [0002] Obesity is a complex metabolic disorder determined by multiple factors, and its incidence is on the rise. According to the report of the World Health Organization, about 250 million adults worldwide suffer from obesity. According to statistics, the annual cost of treating obesity in developed countries has reached 2%-7% of the total medical expenses, and obesity has become an urgent problem to be solved. Fundamentally, obesity results from a disruption of the balance between food intake and energy expenditure. At present, most of the weight-loss drugs with definite clinical efficacy are chemically synthesized drugs, such as noradrenaline uptake inhibitors, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61P3/04
Inventor 王桥夏彬彬赵文华宋学英
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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