Preparation and application of skin-photoage-resisting Chinese herbal compound extractive

A skin photoaging and extract technology, which is applied in skin care preparations, skin diseases, drug combinations, etc., can solve the problems of slow anti-skin photoaging effect and inconvenient long-term taking of traditional Chinese medicine, and achieve enhanced pharmacological activity and small size , high bioavailability

Inactive Publication Date: 2013-10-30
王业秋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] One object of the present invention is to provide a Chinese medicine compound extract with anti-skin photoaging effect and its preparation method

Method used

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  • Preparation and application of skin-photoage-resisting Chinese herbal compound extractive
  • Preparation and application of skin-photoage-resisting Chinese herbal compound extractive
  • Preparation and application of skin-photoage-resisting Chinese herbal compound extractive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 (CO 2 Protective effect of supercritical extract on UV damaged skin cells)

[0043] 1 Experimental materials and instruments

[0044] Human skin fibroblast ESF-1 (Beijing Union Medical College Cell Center), human skin keratinocyte HaCaT (Wuhan University Cell Center), MEM medium, DMEM medium (Gibco Company), fetal bovine serum, trypsin, double antibody ( Billab), MTT (Sigma), vitamin E (Shanghai Jinban Pharmaceutical Co., Ltd.), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA ) assay kit (Nanjing Jiancheng Biological Engineering Company). Ultraviolet irradiation instrument (Sigma Company), ultraviolet irradiation meter (Beijing Normal University Photoelectric Instrument Factory), Resecarch UV UF ultrapure water preparation system (Shanghai Hetai Instrument Co., Ltd.), LDZX-4DSBI vertical automatic electric heating pressure steam extinguisher Sterilizer (Shanghai Shen'an Medical Instrument Factory), IX-71-21PH Olympus invert...

Embodiment 2

[0063] Example 2 (CO 2 Experimental study on the effect of supercritical extract on the skin of photoaging rats)

[0064] 1 Experimental materials and instruments

[0065] 40 healthy Wistar rats, half male and half male, weighing 180±20 g, were provided by the GLP Center of Heilongjiang University of Traditional Chinese Medicine. Vitamin E (Shanghai Jinban Pharmaceutical Co., Ltd.).

[0066] SS-03AB UV phototherapy instrument (Sigma Company), ultraviolet radiation meter (Beijing Normal University Photoelectric Instrument Factory), rat holder (Suzhou Fengshi Experimental Animal Equipment Co., Ltd.), Histoslide 2000 microtome (Reichert-Jung , Germany), LS-70 baker (Shenyang Longshou Electronic Instrument Co., Ltd.), YB1201DW electronic balance (Shanghai Haikang Electronic Instrument Factory), CX-31 Olympus biological microscope (Japan Olympus Co., Ltd. ), TGL-16G-C high-speed refrigerated centrifuge (Shanghai Anting Scientific Instrument Factory), MS-500A semi-automatic bioch...

Embodiment 3

[0097] Embodiment 3 (preparation containing Chinese medicine compound extract external preparation)

[0098] In this embodiment, the external preparation is prepared by using the compound extract of traditional Chinese medicine, and the formula is shown in Table 3 (but not as a limitation of the present invention, the excipients of conventional external preparations in the field are all suitable for the technical solution of the present invention).

[0099] Preparation:

[0100] Oil phase:

[0101] 1. Add argan oil, polydimethylsiloxane, isooctyl palmitate, squalane, 16-18 alcohol, and propylparaben in sequence in the oil phase tank, stir and heat up to 80°C to dissolve them all .

[0102] 2. Add wheat emulsifier E, oil-soluble azone, and bisabolol in sequence, and stir until completely dissolved at 80°C.

[0103] 3. Add polyacrylamide / C13-14 isoparaffin / laurane, extract, stir well at 80°C.

[0104] water box:

[0105] Add purified water to the water phase tank and raise ...

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Abstract

The invention relates to preparation and application of a skin-photoage-resisting Chinese herbal compound extractive. According to the Chinese herbal compound extractive, ten traditional Chinese medicines namely angelica sinensis, ligusticum wallichii, bighead atractylodes rhizome, poria cocos, astragalus membranaceus, codonopsis pilosula, peach kernel, Chinese yam, radix paeoniae alba and honey-fried licorice root are taken as raw materials, and the skin-photoage-resisting Chinese herbal compound extractive is extracted and prepared through a supercritical CO2 extraction technique according to certain technological parameters. The preparation method is simple, the conventional purification and refining process in extraction is omitted, the original characteristics of all the components are furthest maintained, effective components are highly concentrated, and the bioavailability is high. The extractive has remarkable effects of scavenging free radical, resisting oxidative damage and enhancing skin elasticity, and can be directly and safely used for preparing anti-skin photoage external preparations and cosmetics.

Description

technical field [0001] The present invention relates to a compound extract of traditional Chinese medicine, in particular to a kind of traditional Chinese medicine which uses ten kinds of Chinese medicines as raw materials, including Angelica sinensis, Chuanqiong, Atractylodes macrocephala, Poria cocos, Astragalus root, Codonopsis pilosula, peach kernel, Chinese yam, white peony root and roasted licorice. 2 The extract prepared by supercritical extraction technology has obvious anti-skin photoaging effect, and the invention also relates to the application of the extract in external preparations and cosmetics for anti-skin photoaging. Background technique [0002] In recent years, with the rapid development of the global economy, environmental pollution has continued to intensify, and the degree of ozone layer damage has become increasingly serious. The formation of the ozone hole has significantly increased the ultraviolet rays radiated to the earth's surface. Long-term expo...

Claims

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Application Information

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IPC IPC(8): A61K36/8945A61K8/97A61P17/18A61Q19/08
Inventor 王业秋陈巧云鲁光宝祁永华
Owner 王业秋
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