Acylase for producing 7-ACA (Aminocephalosporanic acid) by splitting CPC (Cephalosporin C) by utilizing a one-step method and polynucleotide encoding same

A technology of acylase and acylase activity, applied in the field of biochemical pharmaceuticals, can solve the problems of low substrate specificity, poor substrate tolerance, low conversion efficiency, etc.
CN103374558AActive Publication Date: 2013-10-30SHANGHAI INST OF PHARMA IND +1
2 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI INST OF PHARMA IND
Publication Date
2013-10-30

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an acylase for producing 7-ACA (Aminocephalosporanic acid) by splitting CPC (Cephalosporin C) by utilizing a one-step method, polynucleotide encoding the same, a recombinant expression vector containing a nucleotide sequence, a recombinant expression transformant containing the nucleotide sequence, a preparation method of the acylase and an application of a recombinase in synthesis of the 7-ACA. A CPC acylase is a protein of (a) or (b) as follows: (a) a protein which has CPC acylase activity and an amino acid sequence represented by SEQ ID NO.3, wherein 180-site valine is substituted; and (b) a protein which has the CPC acylase activity and an amino acid sequence formed by adding one or multiple amino acids to an N terminal or a C terminal of the amino acid sequence in the (a) and is derivative from (a). According to the CPC acylase disclosed by the invention, both the enzyme activity and the substrate tolerance are improved, so that a strong precondition is provided for manual determinated evolution transformation of the CPC acylase.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biochemical pharmacy, and particularly relates to a CPC acylase and its gene for producing 7-ACA by splitting cephalosporin C (CPC) in one step, a recombinant expression vector containing the gene, a recombinant expression transformant, and the CPC Preparation method of acylase and its application in the synthesis of 7-aminocephalosporanic acid. Background technique

[0002] 7-Aminocephalosporanic acid (7-ACA), as a key intermediate of semi-synthetic cephalosporin antibiotics, is of great value in the pharmaceutical industry. In industry, chemical method and two-step enzymatic method are often used to produce 7-ACA by using cephalosporin C (Cephalosporin C, CPC). However, the process conditions of the chemical method are relatively harsh, and a variety of toxic substances need to be processed, causing serious environmental pollution. Although a new two-step enzymatic process is being widely adopted, CPC is first ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More