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Devosia sp and application thereof in degrading vomitoxin

A DeVosella technology is applied to a strain of DeVosella and its application field of degrading vomitoxin to achieve the effects of solving the problem of toxin pollution, degrading safety, and low production and use costs

Active Publication Date: 2013-11-13
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous emergence of the disadvantages of physical and chemical detoxification methods and the increasing awareness of the advantages of rapidly developing biotechnology, research on the use of microorganisms for toxin detoxification is gradually being carried out at home and abroad, although there are not many research results on the use of microorganisms for detoxification , but the existing achievements have shown good development and application prospects

Method used

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  • Devosia sp and application thereof in degrading vomitoxin
  • Devosia sp and application thereof in degrading vomitoxin
  • Devosia sp and application thereof in degrading vomitoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Devosia sp. (Devosia sp.) DDB001 acquisition and identification

[0028]Soil samples were collected from a wheat field in Nanyang, Henan, and a 96-microwell plate was used as a culture carrier. First, a bacterial suspension capable of degrading vomitoxin was obtained through six consecutive toxin concentration gradient enrichment culture methods, and then the bacterial suspension was diluted by Coating method Spread on TY agar plate (yeast extract 4g / L, tryptone 8g / L, agar powder 15g / L, pH 7.2, sterilize at 121°C for 30min) with appropriate dilution, pick the degree of separation Colonies with good colony shape and different colors were detoxified in TY liquid medium (yeast extract 4g / L, tryptone 8g / L, pH7.2, sterilized at 121°C for 30min) with a DON concentration of 200μg / mL In the experiment, ethyl acetate extracted the residual toxin and carried out HPLC detection to verify the toxin degradation effect of each pure culture, and finally successfully obtained...

Embodiment 2

[0037] Example 2 Detoxification effect of Devosia sp. DDB001 in TY medium

[0038] The activated DDB001 was inoculated into 50 mL of TY liquid medium containing 200 μg / mL vomitoxin at an inoculation amount of 1%, and detoxified for two days under shaking conditions of 200 rpm, and extracted with ethyl acetate Residual deoxynivalenol, the residual content of deoxynivalenol in the final culture was detected by HPLC: the deoxynivalenol in TY was completely degraded, with a degradation rate of 100%, and toxin metabolites with a retention time of about 9 minutes (such as image 3 shown).

Embodiment 3

[0039] Example 3 Preparation of Devosia sp. DDB001 liquid bacterial agent

[0040] Solid medium components and proportions: yeast extract 0.8%, peptone 0.4%, NaCl 0.5%, agar powder 1.5%, pH7.2–7.4, high temperature steam sterilization at 121°C for 30min.

[0041] Components and proportions of seed medium: yeast extract 0.8%, peptone 0.4%, glucose 0.2%, sodium chloride 0.5%, pH7.2–7.4; fermentation medium components and proportions: yeast extract 0.8%, peptone 0.4 %, Glucose 0.2%, NaCl 0.5%, K 2 HPO 4 ·3H 2 O0.3%, KH 2 PO 4 0.075g / L, pH7.2–7.4.

[0042] (1) Strain activation: Inoculate De Vosella DDB001 with the preservation number CGMCC No.7185 on a solid medium, culture it at 30°C for 3 days, measure its toxin degradation performance, and then inoculate it on the inclined surface of the test tube on standby.

[0043] (2) Seed culture: Pick a single colony from the slant medium and insert it into the seed medium, and cultivate it to the logarithmic phase at 30°C to obta...

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Abstract

The invention discloses devosia sp. and application thereof in degrading vomitoxin. The strain provided by the invention belongs to a new strain in the Devosia Nakagawa et al, and the preservation number of Devosia sp. DDB001 is CGMCC (China General Microbiological Culture Collection Center) No.7185. The invention also provides a fungicide containing the preserved strain and a preparation method thereof. Furthermore, the invention also provides an application of the strain or fungicide in degrading vomitoxin.

Description

technical field [0001] The present invention relates to a De Vosella strain and its application in degrading vomitoxin. Background technique [0002] Deoxynivalnol (deoxynivalnol, DON), the chemical name is 3α,7α,15-trihydroxy-12,13-epoxytrichothecene-9-en-8-one, which is mainly composed of cereal Trichothecenes produced by Fusarium graminearum and Fusarium culmorum infecting wheat, barley, oats, corn and other grains, because this substance can cause vomiting symptoms in animals, it is also called vomitoxin ( vomitoxin). Worldwide, DON is one of the main contaminating mycotoxins in grain, feed, and food, seriously affecting the health of humans and livestock. After humans and animals ingest food contaminated by vomitoxin, it will cause acute poisoning symptoms such as anorexia, vomiting, diarrhea, fever, unsteady standing, and unresponsiveness. In severe cases, it will damage the hematopoietic system and cause death. General attention. [0003] The pollution of vomitoxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/00A23L1/015A23L1/105C12R1/01A23L5/20A23L7/104
Inventor 汪洋
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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