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Induction method for embryogenesis and plant regeneration of in vitro haploid of macleaya cordata anther

A technology of embryogenesis and regeneration, which is applied in the field of plant tissue culture, can solve the problem that the reproduction method cannot meet the market demand, and achieve the effect of low pollution rate and high callus induction rate

Active Publication Date: 2013-11-27
HUNAN AGRICULTURAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for producing haploid seedlings of Boluohui by using tissue culture technology in view of the contradiction in the market that the differentiation of hereditary traits of the offspring and the reproduction mode of the offspring cannot meet the market demand when using seeds to breed high-quality Boluohui seed resources. The method overcomes the defects existing in existing breeding methods, facilitates rapid, efficient, and large-scale breeding of genetically uniform and healthy high-quality Boluohui germplasm resources, and is applied to Boluohui breeding and genomics research

Method used

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  • Induction method for embryogenesis and plant regeneration of in vitro haploid of macleaya cordata anther
  • Induction method for embryogenesis and plant regeneration of in vitro haploid of macleaya cordata anther
  • Induction method for embryogenesis and plant regeneration of in vitro haploid of macleaya cordata anther

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) The preparation of the medium, including the basic medium and the components and proportions of the medium in each stage of tissue culture:

[0032] 1) Basic medium: There are two kinds of basic medium, BLCG and MS, in which 30g / L sucrose, 7g / L agar, and pH5.8 are added respectively;

[0033]2) Embryogenic callus medium: BLCG+2,4-D0.5mg / L+6-BA0.5mg / L;

[0034] 3) Embryoid body induction medium: MS+2,4-D0.5mg / L+6-BA0.5mg / L;

[0035] 4) Somatic embryo germination and seedling growth medium: MS+GA 3 0.5mg / L;

[0036] (2) Anther tissue somatic embryogenesis:

[0037] 1) Collection and disinfection of explants: Take the emerald-green bods that are in the full flowering stage, 2-12 mm in length, and fall back to the flower buds (the anther part where most of the microspore cells are in the single-nucleated marginal stage is observed under a microscope, see the attached figure 1 ), cleaned with distilled water, and rinsed with sterile water on an ultra-clean workbench;...

Embodiment 2

[0043] This embodiment adopts embryogenic callus culture medium: 2, 4-D takes 1.0, 1.5mg / L, 2.0, 2.5, 3.0mg / L respectively; the rest of the implementation steps are the same as in Example 1, all of which can achieve the results of Example 1. Effect.

Embodiment 3

[0045] This embodiment adopts embryoid body induction medium: 2, 4-D take 0.8, 1.0 mg / L respectively; the rest of the implementation steps and techniques are the same as in Example 1, and the effect of Example 1 can be achieved.

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Abstract

The invention discloses an induction method for embryogenesis and plant regeneration of in vitro haploid of macleaya cordata anther, which belongs to the field of plant tissue culture techniques. The method comprises the following steps: (1) preparation of a medium; (2) somatic embryogenesis; (3) germination and seedling formation of a somatic embryo; and (4) domestication, transplantation and the like of a tissue culture seedling. According to the invention, the induction method is a tissue culture method established with the macleaya cordata anther as an explant and can realize rapid, high-efficiency and large-scale production of high-quality macleaya cordata haploid plants; after field screening, the macleaya cordata haploid plants can be directly used as breeding materials for research on breeding and genomics.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing haploid embryogenesis and plant regeneration in isolated anthers. Background technique [0002] Macleaya cordata (Willd) R. Br is a plant of the genus Macleaya cordata (Willd) R. Br, which is rich in a variety of isoquinoline alkaloids, such as sanguinarine (sanguinarine, SA), chelerythrine (chelerythrine , CHE), protopine (protopine, PRO) and allocryptopine (allocryptopine, ALL), etc. (Guo Yuge, Zeng Jianguo, Tan Manliang, etc. 4 species Content comparison of alkaloids. Zhongnan Pharmacy. 2011, 9 (11): 829-832). They have significant and rich biological activity and pharmacological activity, and they play an important role in regulating the intestinal flora of pigs (Cai Peng, Sun Zhiliang, Zeng Jianguo, et al. Effects of different doses of Boluohui extract on growth performance of weaned piglets. Chinese Animal Husbandry and Veterinary Medicin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 曾建国李炎林柳亦松宋锡帅芦强
Owner HUNAN AGRICULTURAL UNIV
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