Application of oxidized resveratrol for preparing tumor treatment drugs

A technology for oxidizing resveratrol and tumors, which is applied in the direction of antitumor drugs, drug combinations, and active ingredients of hydroxyl compounds, etc. The effect of low side effects and obvious social benefits

Inactive Publication Date: 2013-12-04
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In vitro experiments confirmed that oxidized resveratrol is a tyrosinase inhibitor, which can inhibit the release of histamine from basophilic cells (Kim D H, Kim J H, Baek S H.Enhancement of tyrosinase inhibition of the extract of veratrum patulum using cellulase.Biotechnology and Bioengineering, 2004, 87(7):849-854), w

Method used

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  • Application of oxidized resveratrol for preparing tumor treatment drugs
  • Application of oxidized resveratrol for preparing tumor treatment drugs
  • Application of oxidized resveratrol for preparing tumor treatment drugs

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0035] Example 1: In vitro anti-tumor effect of oxidized resveratrol

[0036] 1. Experimental materials

[0037] 1.1 Cell lines: human leukemia cell K562, human ovarian cancer cell SK-OV-3, human breast cancer cell MCF-7, human liver cancer cell SMMC-7721, human lung cancer cell A549, human nasopharyngeal carcinoma cell HNE-1, human colon The cancer cells SW480 were purchased from Shanghai Cell Bank and cryopreserved by the Department of Pharmacology, School of Basic Medicine and Forensic Medicine, Sichuan University.

[0038] 1.2 Test drugs and positive control drugs

[0039] 1.2.1 Test drug: Oxidized resveratrol, purity ≥98%, purchased from Tixiai Chemical Industry (Shanghai) Co., Ltd.;

[0040] 1.2.2 Positive control drug: Doxorubicin Hydrochloride for Injection (Adriamycin), Zhejiang Hisun Pharmaceutical Co., Ltd., batch number 090204A.

[0041] 1.3 Reagents and preparation

[0042] RPMI-1640 culture medium, Chengdu Harry Biological Company sub-packed; Calf serum, Chengdu Harry Biolo...

Example Embodiment

[0099] Example 2: The inhibitory effect of oxidized resveratrol on the growth of transplanted tumor S180 sarcoma in mice

[0100] 1. Experimental materials

[0101] 1.1 Experimental animals: Kunming mice, male, weighing 18-22g, a total of 180, animal certificate SCXK (Sichuan) 2008-24, purchased from Chengdu Dashuo Biotechnology Co., Ltd.

[0102] 1.2 Murine-derived S180 sarcoma cells: purchased from Shanghai cell bank and cryopreserved by the Department of Pharmacology, School of Basic Medicine and Forensic Medicine, Sichuan University.

[0103] 1.3 Experimental drugs and reagents

[0104] 1.3.1 Test drug: Oxidized resveratrol, purity ≥98%, purchased from Tixiai Chemical Industry (Shanghai) Co., Ltd.;

[0105] 1.3.2 Positive control drug: cyclophosphamide powder injection, purchased from Jiangsu Hengrui Pharmaceutical Co., Ltd., batch number 11030621;

[0106] 1.3.3 Reagents

[0107] 0.9% sodium chloride injection (normal saline): purchased from Chengdu Qingshan Likang Pharmaceutical Co.,...

Example Embodiment

[0166] Example 3: The inhibitory effect of oxidized resveratrol on the growth of transplanted tumor H22 liver cancer in mice

[0167] 1. Experimental materials

[0168] 1.1 Experimental animals: Kunming mice, male, weighing 18-22g, a total of 180, animal certificate SCXK (Sichuan) 2008-24, purchased from Chengdu Dashuo Biotechnology Co., Ltd.

[0169] 1.2 Mouse-derived H22 liver cancer cells: purchased from Shanghai Cell Bank, and cryopreserved by the Department of Pharmacology, School of Basic Medicine and Forensic Medicine, Sichuan University.

[0170] 1.3 Experimental drugs and reagents

[0171] 1.3.1 Test drug: Oxidized resveratrol, purity ≥98%, purchased from Tixiai Chemical Industry (Shanghai) Co., Ltd.;

[0172] 1.3.2 Positive control drug: cyclophosphamide powder injection, purchased from Jiangsu Hengrui Pharmaceutical Co., Ltd., batch number 11030621;

[0173] 1.3.3 Reagents

[0174] 0.9% sodium chloride injection (normal saline): purchased from Chengdu Qingshan Likang Pharmaceuti...

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Abstract

The invention discloses an application of oxidized resveratrol for preparing tumor treatment drugs. Extracorporeal cell experiments indicate that the oxidized resveratrol has good growth inhibition effects on seven human source tumor cell strains including human leukemia cells K562, human ovarian carcinoma cells SK-OV-3, human breast cancer cells MCF-7, human hepatoma carcinoma cells SMMC-7721, human lung cancer cells A549, human nasopharyngeal carcinoma cells HNE-1 and human colon cancer cells SW480; the IC50 value of the oxidized resveratrol within 48 hours is between 0.047mM and 0.277mM; the growth inhibition effects on the human hepatoma carcinoma cells SMMC-7721, the human ovarian carcinoma cells SK-OV-3 and the human breast cancer cells MCF-7 are particularly obvious. Animal experiments indicate that the oxidized resveratrol has obvious inhibition effects on the growth of mouse transplanted tumor S180 sarcoma and mouse transplanted tumor H22 liver cancer, and is low in toxic and side effect.

Description

technical field [0001] The present invention relates to the use of oxidized resveratrol, especially in medicine. Background technique [0002] Tumor is a disease that seriously endangers human health. It is a tangible tumor mass that can be detected clinically by means of X-rays, CT scans, B-ultrasound or palpation. It can be divided into two types: benign and malignant. Malignant tumor mass is also commonly referred to as cancer, and the common clinical malignant solid tumors include lung cancer, neuroma, colorectal cancer, and sarcoma. [0003] At present, more than 1.5 million people die of cancer every year in my country, and on average, 1 out of every 5 deaths is due to cancer. In the past 30 years, the incidence of cancer in the world has increased by an average of 3% to 5% per year. In my country, the overall incidence and mortality of cancer have increased significantly in the past 20 years. [0004] At present, the treatment of cancer is still based on the compreh...

Claims

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Application Information

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IPC IPC(8): A61K31/05A61P35/00A61P35/02
Inventor 周黎明王锋鹏熊尧蒋洪武万莉红张媛媛
Owner SICHUAN UNIV
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