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Preparation method for modified cytidine antibiotic

A technology of cytidine and chemical modification is applied in the field of preparation of modified cytidine, which can solve the problem of no antibacterial activity of mothomycin, and achieve the effects of environment-friendly production process, good chemical selectivity and broad application prospect.

Inactive Publication Date: 2013-12-04
徐州诺特化工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the antifungal activity of 2-clonopyrrolidin is only 10% of that of nipyrrolidin (contains two chlorine atoms); compared with its chlorine-containing derivatives, the non-chlorinated tritemycin has no antibacterial activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Strain: S510 strain obtained after natural isolation of Streptomyces hygroscopicus varietal Hainan strain S101 was used as the starting strain.

[0019] Medium: 20g corn flour, 25g bean cake powder, 18g glucose, 10g starch, 3g ammonium chloride, 3g calcium carbonate, 0.3 magnesium sulfate, 0.3 ferrous sulfate, 0.3g sodium chloride, 0.3g disodium hydrogen phosphate, add tap water to 1000mL. Sterilize at 121°C for 20 minutes.

[0020] Fermentation culture: shaker culture, rotation speed: 220 rpm, fermentation temperature: 28°C, tank pressure 0.12MPa, ventilation rate 1:0.5, fermentation time 72h.

[0021] Separation and purification of fermentation products: extraction of fermentation broth, chromatography on Sephadex G-25 column, elution with water, and detection of eluate by HPLC (COSMOSIL C18 Econopak column, mobile phase 0.03% trifluoroacetic acid aqueous solution, flow rate 0.4mL / min , ultraviolet detection wavelength 220nm), and the main components whose retention...

Embodiment 2

[0024] Strain: S510 strain obtained after natural isolation of Streptomyces hygroscopicus varietal Hainan strain S101 was used as the starting strain.

[0025] Fermentation medium: corn flour 25g, bean cake powder 35g, glucose 15g, starch 12g, ammonium chloride 5g, calcium carbonate 5g, magnesium sulfate 0.1, ferrous sulfate 0.1, sodium chloride 0.1g, disodium hydrogen phosphate 0.5g, add Tap water to 1000mL. Sterilize at 121°C for 20 minutes.

[0026] Fermentation culture: shaking culture on a shaking table, the rotation speed is 250 rpm, the fermentation temperature is 25°C, the tank pressure is 0.15MPa, the ventilation rate is 1:0.5, and the fermentation time is 64h.

[0027] Separation and purification of fermentation products: extraction of fermentation broth, chromatography on Sephadex G-25 column, elution with water, and detection of eluate by HPLC (COSMOSIL C18 Econopak column, mobile phase 0.03% trifluoroacetic acid aqueous solution, flow rate 0.4mL / min , ultraviole...

Embodiment 3

[0030]Strain: S510 strain obtained after natural isolation of Streptomyces hygroscopicus varietal Hainan strain S101 was used as the starting strain.

[0031] Fermentation medium: 15g corn flour, 35g bean cake powder, 15g glucose, 8g starch, 1g ammonium chloride, 1g calcium carbonate, 0.5 magnesium sulfate, 0.5 ferrous sulfate, 0.5g sodium chloride, 0.1g disodium hydrogen phosphate, add Tap water to 1000mL. Sterilize at 121°C for 20 minutes.

[0032] Fermentation culture: shaking culture on a shaking table, the rotation speed is 250 rpm, the fermentation temperature is 35°C, the tank pressure is 0.01MPa, the ventilation rate is 1:0.5, and the fermentation time is 84h.

[0033] Separation and purification of fermentation products: extraction of fermentation broth, chromatography on Sephadex G-25 column, elution with water, and detection of eluate by HPLC (COSMOSIL C18 Econopak column, mobile phase 0.03% trifluoroacetic acid aqueous solution, flow rate 0.4mL / min , ultraviolet ...

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Abstract

Provided is a modified cytidine antibiotic. The modified cytidine antibiotic is a nitrogen substituting cytidine antibiotic with a side chain -C4H8ClO9. The preparation method comprises steps: streptomyces ahygroscopicus is taken as a starting strain and subjected to fermentation cultivation in medium; after separation, purification and chemical modification, the modified cytidine antibiotic is obtained. The modified cytidine antibiotic has high biological resistance and structural stability. The preparation method has good chemoselectivity and mild technology conditions, and production processes are environmentally friendly.

Description

[0001] Technical field: the invention belongs to the technical field of microbial pesticides, and relates to a preparation method of modified cytidine. [0002] Background technique: [0003] Agricultural antibiotics, referred to as agricultural antibiotics, refer to secondary metabolites produced by microbial fermentation, which have the function of pesticides, and are used in agriculture to control pests, weeds, rats and other harmful organisms. Compared with general chemically synthesized pesticides, agricultural antibiotics have the following characteristics: ①complex structure; ②high activity, small dosage, and good selectivity; ③easy to be decomposed by biological or natural factors, and do not accumulate or remain in the environment; ④production raw materials are Agricultural products such as starch and sugar belong to renewable energy sources; ⑤ Fermentation engineering is used for production. The same set of equipment can produce different antibiotics only by changing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/38C07H19/06C07H1/00C12R1/55
Inventor 王玉成高玉忠王理想王友志杨凯曹再林张春来靳长娟
Owner 徐州诺特化工有限公司
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