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Method for preparing ethanol by adding Mn (II) salt for promoting simultaneous saccharification and fermentation of straw

A saccharification fermentation and ethanol technology, which is applied in biochemical equipment and methods, microorganism-based methods, fermentation and other directions, can solve the problems of insufficient utilization of enzymatic hydrolysis products, unsatisfactory ethanol yield, and increased production energy consumption, etc. Achieve the effect of improving final yield, low production cost and reducing energy consumption

Inactive Publication Date: 2013-12-11
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method mainly has the following disadvantages: (1) adopts step-by-step saccharification and fermentation method to prepare ethanol, has increased process steps (as filtration and decolorization of enzymolysis liquid), and operation is comparatively complicated, and ethanol production rate is not ideal enough
(2) Using a single yeast for fermentation, the enzymatic hydrolysis product is not fully utilized, which is also one of the reasons for the low ethanol yield
(3) The process cycle is longer, which increases the production energy consumption and also increases the cost
In addition, the application number is 201210597201.5, and the title is "A New Method for Improving the Yield of Fuel Ethanol Made from Rice Straw". lower

Method used

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Examples

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Effect test

Embodiment 1

[0023] Adding Mn (II) salt to promote the method for preparing ethanol by simultaneous saccharification and fermentation of rice straw, its specific steps are as follows:

[0024] (1) Rice straw pretreatment

[0025]The rice straw was manually cut, ground by a ball mill and passed through an 80-mesh sieve, and then dried to a constant weight; 20g of dry straw and 120g of ethylene glycol were weighed into a three-necked flask and put into a magnetic rotor; after the distillation device was installed, the three-necked The middle port of the flask is connected with the condenser tube, put it into a magnetic water bath stirrer, set the reaction temperature to 90°C, and the stirring rate to 100r / min, and start stirring. Insert the hole into the reaction mixture, and add a non-porous rubber plug at the last port as the feed port; pull out the rubber plug of the feed port after preheating for 10 minutes, add 25 mmol of concentrated sulfuric acid and plug it, and stir at a constant te...

Embodiment 2

[0033] Adding Mn (II) salt to promote the method for preparing ethanol by simultaneous saccharification and fermentation of rice straw, its specific steps are as follows:

[0034] In step (1): Grind the rice straw and pass it through an 80-mesh sieve, dry the rice straw to 15 g, and ethylene glycol to 110 g, set the reaction temperature to 85°C, stir at a rate of 90 r / min, add 20 mmol of concentrated sulfuric acid for preheating for 5 minutes , stirred at a constant temperature for 60 min, and the reactant was suction-filtered with 15 mL of methanol for a second suction filtration, washed with hot water and suction-filtered twice, 15 mL each time, and dried to obtain a fermentation substrate.

[0035] In step (2): Glucose 10g, peptone 15g, yeast powder 5g, distilled water 0.8L, 110°C damp heat sterilization for 15min, two kinds of yeast cultured on slant planes were respectively activated in liquid medium (30°C, 16h), and used The hemocytometer plate was loaded with the stirre...

Embodiment 3

[0039] Adding Mn (II) salt to promote the method for preparing ethanol by simultaneous saccharification and fermentation of rice straw, its specific steps are as follows:

[0040] In the first step (1): Grind the rice straw and pass it through an 80-mesh sieve, dry the rice straw to 25 g, ethylene glycol to 130 g, set the reaction temperature to 95°C, stir at a rate of 110 r / min, add 30 mmol of concentrated sulfuric acid for 15 minutes of preheating , stirred at constant temperature for 80min, after the reactant was suction filtered, 25mL of methanol was added for secondary suction filtration, and finally washed with hot water and suction filtered twice, 25mL each time, and the fermentation substrate was obtained after drying.

[0041] In step (2): 30g of glucose, 25g of peptone, 15g of yeast powder, 1.2L of distilled water, 120°C damp heat sterilization for 25min, respectively take two kinds of yeast cultured on a slope and activate them in liquid medium (30°C, 20h). The hemo...

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Abstract

The invention provides a method for preparing ethanol by adding Mn (II) salt for promoting simultaneous saccharification and fermentation of straw. The method comprises the steps of: after grinding and sieving straw stalks, selecting glycol as a liquefier, carrying out liquefaction pretreatment under the catalysis action of concentrated sulfuric acid, and carrying out washing and suction filtration to obtain a fermentation substrate; selecting pachysolen tannophilus and saccharomyces cerevisiae as fermentation microorganisms, respectively taking the two types of yeast after slant culture and activating in a YEPD (Yeast Extract Peptone Dextrose) liquid culture medium, loading a culture medium mixed liquor by using a hemocytometer, determining the yeast concentration through microscopic counting, and diluting to suitable concentration; respectively adding the fermentation substrate, a MnSO4 solution, cellulase and the diluted yeast mixed liquor into a fermentation tank, and after adjusting to a suitable solid-liquid mass ratio by using sterile water, carrying out simultaneous saccharification and fermentation under the condition of constant temperature; finally determining the ethanol concentration in fermentation liquor by using a spectrophotometric method, and calculating to obtain the final yield of the ethanol. According to the method, the related raw materials are wide in sources, and the method is convenient to operate, and can be used for reducing the dosage of the cellulase so as to lower the process cost and starting biochemical reaction at low temperatures, thus providing a new train of thought for a production process in the field.

Description

technical field [0001] The invention belongs to the technical field of new energy preparation methods, and in particular relates to a method for preparing ethanol from fibrous materials. Background technique [0002] On a global scale, countries all over the world are facing the depletion of fossil energy and the growing population. Finding new, renewable and clean alternative energy has become one of the most urgent tasks for all mankind. Bioethanol is considered to be the most important renewable alternative fuel for vehicles in the next 20 years, and it has begun to be used on a large scale in Brazil, the United States and some European countries. Bioethanol has unparalleled advantages compared to other fuels, such as wide source of raw materials, low price, miscible with gasoline in any ratio, increasing the oxygen content in gasoline to promote the full combustion of gasoline, non-toxic side effects and convenient application in current engines superior. The current b...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/10C12P19/14C12R1/645C12R1/865
CPCY02E50/10
Inventor 徐龙君毕智明谢太平刘成伦王金成
Owner CHONGQING UNIV
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