Method for preparing ethanol by adding Mn (II) salt for promoting simultaneous saccharification and fermentation of straw

A saccharification fermentation and ethanol technology, which is applied in biochemical equipment and methods, microorganism-based methods, fermentation and other directions, can solve the problems of insufficient utilization of enzymatic hydrolysis products, unsatisfactory ethanol yield, and increased production energy consumption, etc. Achieve the effect of improving final yield, low production cost and reducing energy consumption

Inactive Publication Date: 2013-12-11
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method mainly has the following disadvantages: (1) adopts step-by-step saccharification and fermentation method to prepare ethanol, has increased process steps (as filtration and decolorization of enzymolysis liquid), and operation is comparatively complicated, and ethanol production rate is not ideal enough
(2) Using a single yeast for fermentation, the enzymat

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0022] Example 1

[0023] The method of adding Mn(II) salt to promote simultaneous saccharification and fermentation of rice straw to produce ethanol, the specific steps are as follows:

[0024] (1) Pretreatment of straw

[0025] After the straw is manually sheared, pulverized by a ball mill and passed through an 80-mesh sieve, it is dried to a constant weight; 20g of dried straw and 120g of ethylene glycol are weighed into a three-necked flask and put into the magnetic rotor; the distillation device is installed and the three-necked The middle mouth of the flask is connected with the condenser, put in a magnetic water bath stirrer, set the reaction temperature to 90℃, stirring rate 100r / min, start stirring, add a rubber stopper with a hole on the side of the flask, and pass the thermometer through the small Insert the hole into the reaction mixture, and add a non-porous rubber stopper as the feed port at the last port; pull out the rubber stopper at the feed port after preheating f...

Example Embodiment

[0032] Example 2

[0033] The method of adding Mn(II) salt to promote simultaneous saccharification and fermentation of rice straw to produce ethanol, the specific steps are as follows:

[0034] In step (1): grind the rice straw and pass through an 80-mesh sieve, dry rice straw mass 15g, ethylene glycol mass 110g, set the reaction temperature to 85℃, stirring rate 90r / min, preheat for 5min, add 20mmol concentrated sulfuric acid , Stir at constant temperature for 60 minutes, the reactant is suction filtered and then filtered with 15mL methanol twice, washed with hot water and filtered twice, 15mL each time, and dried to obtain the fermentation substrate.

[0035] In step (2): glucose 10g, peptone 15g, yeast powder 5g, distilled water 0.8L, 110℃ humid heat sterilization for 15min, respectively take the two kinds of yeast cultured on the slant to activate in the liquid medium (30℃, 16h), use The hemocytometer is loaded with the stirred culture medium (160r·min -1 , 2h), adjust sterile ...

Example Embodiment

[0038] Example 3

[0039] The method of adding Mn(II) salt to promote simultaneous saccharification and fermentation of rice straw to produce ethanol, the specific steps are as follows:

[0040] In step (1): grind the rice straw and pass through an 80-mesh sieve, dry rice straw mass 25g, ethylene glycol mass 130g, set reaction temperature to 95℃, stirring rate 110r / min, preheat 15min and add 30mmol concentrated sulfuric acid , Stir at constant temperature for 80 min, the reactant was suction filtered and 25mL methanol was added for second suction filtration, and finally washed with hot water and suction filtered twice, 25mL each time, and dried to obtain the fermentation substrate.

[0041] In step (2): 30g glucose, 25g peptone, 15g yeast powder, 1.2L distilled water, sterilized at 120°C for 25min with humid heat, respectively take the two kinds of yeast cultured on the slant to activate in liquid medium (30°C, 20h), use The hemocytometer is loaded with the stirred culture medium (1...

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Abstract

The invention provides a method for preparing ethanol by adding Mn (II) salt for promoting simultaneous saccharification and fermentation of straw. The method comprises the steps of: after grinding and sieving straw stalks, selecting glycol as a liquefier, carrying out liquefaction pretreatment under the catalysis action of concentrated sulfuric acid, and carrying out washing and suction filtration to obtain a fermentation substrate; selecting pachysolen tannophilus and saccharomyces cerevisiae as fermentation microorganisms, respectively taking the two types of yeast after slant culture and activating in a YEPD (Yeast Extract Peptone Dextrose) liquid culture medium, loading a culture medium mixed liquor by using a hemocytometer, determining the yeast concentration through microscopic counting, and diluting to suitable concentration; respectively adding the fermentation substrate, a MnSO4 solution, cellulase and the diluted yeast mixed liquor into a fermentation tank, and after adjusting to a suitable solid-liquid mass ratio by using sterile water, carrying out simultaneous saccharification and fermentation under the condition of constant temperature; finally determining the ethanol concentration in fermentation liquor by using a spectrophotometric method, and calculating to obtain the final yield of the ethanol. According to the method, the related raw materials are wide in sources, and the method is convenient to operate, and can be used for reducing the dosage of the cellulase so as to lower the process cost and starting biochemical reaction at low temperatures, thus providing a new train of thought for a production process in the field.

Description

technical field [0001] The invention belongs to the technical field of new energy preparation methods, and in particular relates to a method for preparing ethanol from fibrous materials. Background technique [0002] On a global scale, countries all over the world are facing the depletion of fossil energy and the growing population. Finding new, renewable and clean alternative energy has become one of the most urgent tasks for all mankind. Bioethanol is considered to be the most important renewable alternative fuel for vehicles in the next 20 years, and it has begun to be used on a large scale in Brazil, the United States and some European countries. Bioethanol has unparalleled advantages compared to other fuels, such as wide source of raw materials, low price, miscible with gasoline in any ratio, increasing the oxygen content in gasoline to promote the full combustion of gasoline, non-toxic side effects and convenient application in current engines superior. The current b...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/10C12P19/14C12R1/645C12R1/865
CPCY02E50/10
Inventor 徐龙君毕智明谢太平刘成伦王金成
Owner CHONGQING UNIV
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