Oligosaccharide probiotics composition and application thereof as well as method for preparing capsules by using composition

A technology of probiotics and oligosaccharides, which is applied in the field of biomedicine, can solve the problems of difficult maintenance of bacterial activity, strong dependence on bacterial powder, difficulty in oxygen isolation of granules, etc., and achieve the goals of preventing oxidation, vigorous bacterial vitality, and strengthening health care functions Effect

Active Publication Date: 2014-01-01
中康长和健康科技(深圳)有限公司
View PDF4 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commercially available probiotic products are mainly solid granules, most of which are prepared by mixing freeze-dried bacterial powder and auxiliary materials. Therefore, the product has a strong dependence on bacterial powder, high cost, and lim

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1) Activation of strains

[0029] Inoculate the Lactobacillus that can be used in health food approved by the Ministry of Health into the MRS medium at an inoculation amount of 1ml / 100ml, and culture it at 35°C for 24 hours to obtain activated Lactobacillus strains;

[0030] Among them, the formula of MRS medium is: beef extract 10g, glucose 20g, peptone 10g, yeast extract 5g, manganese sulfate tetrahydrate 0.05g, Tween-801mL, dipotassium hydrogen phosphate 2g, sodium acetate trihydrate 5g, seven Magnesium sulfate water 0.5g, diammonium citrate 2g, distilled water 1000mL;

[0031] 2) Prepare high-bacteria concentrated probiotic liquid

[0032] Inoculate the activated Lactobacillus strains into the mixed culture medium with an inoculum amount of 1ml / 100ml, cultivate at 35°C for 24h, centrifuge at 3000r / min at 4°C for 30min, discard the supernatant, and pour Add concentrated milk to the bacteria slime (the amount of concentrated milk added is 1 / 3 of the volume of the mi...

Embodiment 2

[0040] 1) Activation of strains

[0041] Inoculate the Lactobacillus that can be used in health food approved by the Ministry of Health into the MRS medium at an inoculation amount of 1ml / 100ml, and culture it at 40°C for 18 hours to obtain activated Lactobacillus strains;

[0042] Among them, the formula of MRS medium is: beef extract 10g, glucose 20g, peptone 10g, yeast extract 5g, manganese sulfate tetrahydrate 0.05g, Tween-801mL, dipotassium hydrogen phosphate 2g, sodium acetate trihydrate 5g, seven Magnesium sulfate water 0.5g, diammonium citrate 2g, distilled water 1000mL;

[0043] 2) Prepare high-bacteria concentrated probiotic liquid

[0044] Inoculate the activated Lactobacillus strains into the mixed culture medium with an inoculum amount of 1ml / 100ml. After culturing for 20 hours at 40°C, centrifuge at 4500r / min at 6°C for 20min, discard the supernatant, and pour Add concentrated milk to the bacteria slime (the amount of concentrated milk added is 1 / 3 of the volum...

Embodiment 3

[0052] 1) Activation of strains

[0053] Inoculate the Lactobacillus that can be used in health food approved by the Ministry of Health into the MRS medium at an inoculation amount of 1ml / 100ml, and culture it at 40°C for 16 hours to obtain activated Lactobacillus strains;

[0054]Among them, the formula of MRS medium is: beef extract 10g, glucose 20g, peptone 10g, yeast extract 5g, manganese sulfate tetrahydrate 0.05g, Tween-801mL, dipotassium hydrogen phosphate 2g, sodium acetate trihydrate 5g, seven Magnesium sulfate water 0.5g, diammonium citrate 2g, distilled water 1000mL;

[0055] 2) Prepare high-bacteria concentrated probiotic liquid

[0056] Inoculate the activated Lactobacillus species into the mixed culture medium with an inoculum amount of 1ml / 100ml. After culturing for 24h at 35°C, centrifuge at 5000r / min at 7°C for 15min, and discard the supernatant to precipitate bacteria. Add concentrated milk to the mud (the amount of concentrated milk added is 1 / 3 of the vol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an oligosaccharide probiotics composition and a method for preparing capsules by using the composition, and belongs to the technical field of a biological medicine. The method comprises the following steps: 1) in preparation of 1kg of oligosaccharide probiotics composition, taking 300-600g of oligosaccharide powder, 100-200ml of high-bacterium thick probiotic liquid, and 50-100g of hydroxypropyl methyl cellulose, wherein the oligosaccharide powder is prepared from stachyose, raffinose and jerusalem artichoke flour in the same mass, screening after evenly mixing the oligosaccharide powder and the hydroxypropyl methyl cellulose, and then adding the high-bacterium thick probiotic liquid, so as to obtain the oligosaccharide probiotics composition after fully and evenly mixing; 2) pelletizing the prepared oligosaccharide probiotics composition, and then drying and straightening to obtain oligosaccharide probiotics particulate matters, filling the oligosaccharide probiotics particulate matters into hollow capsules, so as to obtain the oligosaccharide probiotics capsules. The method disclosed by the invention is simple and feasible; the prepared capsules are high in antifungal activity, and have dual effects of oligosaccharides and probiotics.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an oligosaccharide probiotic composition, an application thereof and a method for preparing capsules by using the composition. Background technique [0002] Probiotics have high application value in food, fermentation, medicine and other fields. The activity of probiotics in probiotic products is an important indicator for evaluating their quality. In my country, probiotics are used in dietary supplements, health food or health care products. The research on pharmaceuticals started relatively late. Due to technical problems, it is difficult to maintain the activity of bacteria, so that the health effects of probiotics cannot be truly brought into play. Currently commercially available probiotic products are mainly solid granules, most of which are prepared by mixing freeze-dried bacterial powder and auxiliary materials. Therefore, the product has a strong dependenc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23L1/09A23P1/04C12N1/20C12R1/225A61K35/74A61K9/48A61P1/14A23L33/125A23L33/135A23P10/30A61K35/747
CPCA23L29/30A23P10/30C12N1/20A23V2002/00A23V2250/5108A23V2250/28A23V2400/11A23V2300/40
Inventor 吕嘉枥康婕
Owner 中康长和健康科技(深圳)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap