Preparation method for small-particle-size magnetic agarose microspheres
A technology of agarose microspheres and small particle sizes, applied in the fields of magnetism of organic materials/organic magnetic materials, chemical instruments and methods, alkali metal oxides/hydroxides, etc., can solve the problem of wide particle size distribution and large particle size and other issues, to achieve the effect of wide application prospects and good application prospects
Examples
preparation example Construction
[0027] The preparation method of the small-diameter magnetic agarose microspheres of the present invention comprises the following steps:
[0028] Step 1, the solution formed by dissolving the emulsifier in a hydrophobic organic solvent is used as the oil phase. Wherein, the volume percentage of the emulsifier in the hydrophobic organic solvent is 0.5% to 4%; the hydrophobic organic solvent includes saturated alkanes with a boiling point greater than 90°C. As known to those skilled in the art, the saturated alkanes Including but not limited to n-heptane, n-octane or n-nonane. The emulsifier includes but not limited to Span 85 and Span 80.
[0029] Step 2, agarose is added to the superparamagnetic Fe-containing 3 o 4 In the aqueous solution, the agarose is completely dissolved through vortex oscillation, ultrasonic dispersion, and microwave heating, and the resulting mixed solution is used as the water phase. The superparamagnetic Fe 3 o 4 The surface is covered with a che...
Embodiment 1
[0036] Preparation of the oil phase: Use a 500mL graduated cylinder to measure 400mL of n-octane into a 1000mL three-neck flask, and then add 12mL of Span 85. The three-necked flask was placed in a constant temperature water bath at 95°C, and stirred by electric machinery for 30 minutes.
[0037] Preparation of the aqueous phase: Weigh 4.0g of agarose powder with an analytical balance and add it to a 500mL conical flask, then add 100mL of Fe 3 o 4 / SiO 2 For an aqueous solution with a content of 10 mg / mL, vortex for 3 minutes, and then sonicate for 3 minutes, put the Erlenmeyer flask into a microwave oven and heat to dissolve the agarose.
[0038] Adjust the stirring speed in the oil phase containing n-octane to 1000rpm, slowly add the prepared aqueous phase agarose solution into the oil phase, stir for 2min, and stop stirring; quickly pour the stirred emulsion into the preheated to 100°C~ Place the beaker in a 1000mL beaker at 120°C for 8 minutes in an ultrasonic breaker, ...
Embodiment 2
[0040] Preparation of the oil phase: Use a 500mL graduated cylinder to measure 400mL of n-octane into a 1000mL three-neck flask, and then add 12mL of Span 85. The three-necked flask was placed in a constant temperature water bath at 95°C, and stirred by electric machinery for 30 minutes.
[0041] Preparation of the aqueous phase: weigh 3.0 g of agarose powder with an analytical balance and add it to a 500 mL Erlenmeyer flask, then add 100 mL of Fe 3 o 4 / SiO 2 For an aqueous solution with a content of 10 mg / mL, vortex for 3 minutes, and then sonicate for 3 minutes, put the Erlenmeyer flask into a microwave oven and heat to dissolve the agarose.
[0042] Adjust the stirring speed in the above-mentioned n-octane oil phase to 1500rpm, slowly add the prepared aqueous phase agarose solution into the oil phase, stir for 3min, and stop stirring; quickly pour the stirred emulsion into the preheated to 100~120°C Put the beaker into an ultrasonic breaker for 10 minutes, and then plac...
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Abstract
Description
Claims
Application Information
- IPC
- C08J3/16; C08L5/12; C08K9/10; C08K3/22; B01J20/24; B01J20/28; H01F1/42
- Inventors
- 任辉; 秦炜