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Baicalein molecularly imprinted polymer and its preparation method and application

A technology of molecular imprinting and baicalein, applied in the field of bioengineering, can solve the problems of poor monodispersity and uniformity of particles, destruction of imprinted sites, cumbersome post-processing, etc., and achieve uniform particle size, controllable size, and post-processing and the effect of short cycle

Inactive Publication Date: 2016-04-27
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the synthesized polymer needs to be milled and sieved, a large number of imprinted sites are destroyed, and the monodispersity and uniformity of the particles are poor
At the same time, there are disadvantages of cumbersome post-processing

Method used

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  • Baicalein molecularly imprinted polymer and its preparation method and application
  • Baicalein molecularly imprinted polymer and its preparation method and application
  • Baicalein molecularly imprinted polymer and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Weigh 0.5mmol baicalein, dissolve it in 60mL acetonitrile, add 4.0mmol MAA, sonicate for 10min, and place at room temperature for 2h. Then add TRIM12mmol, AIBN0.066g, sonicate for 10min, nitrogen gas for 10min, seal, 50°C, 150r / min shaking incubator to react for 25h. At the end of the reaction, the baicalein molecularly imprinted polymer was first eluted 10 times with methanol-glacial acetic acid (5:1, V / V), washed 5 times with methanol, and then dried in vacuum at 45°C for 24 hours to obtain the baicalein molecularly imprinted polymer. (MIP-1).

[0030] Except that no template molecules were added during the synthesis process, the rest of the steps were the same as those for the preparation of baicalein molecularly imprinted polymers to obtain non-imprinted polymers (NIP-1).

Embodiment 2

[0032] Weigh 0.5mmol baicalein, dissolve it in 70mL tetrahydrofuran, add 2.5mmol AM, sonicate for 10min, and place at room temperature for 2h. Then add MBA10mmol, AIBN0.056g, sonicate for 10min, nitrogen gas for 10min, seal, 60°C, 200r / min shaking incubator to react for 25h. At the end of the reaction, the baicalein molecularly imprinted polymer was first eluted 10 times with methanol-glacial acetic acid (4:1, V / V), washed 5 times with methanol, and then dried in vacuum at 45°C for 24 hours to obtain the baicalein molecularly imprinted polymer. (MIP-2).

[0033] Except that template molecules were not added during the synthesis process, the rest of the steps were the same as those for the preparation of baicalein molecularly imprinted polymers to obtain non-imprinted polymers (NIP-2).

Embodiment 3

[0035] Weigh 0.5mmol baicalein, dissolve it in 50mL tetrahydrofuran, add 2.5mmol AM, sonicate for 10min, and place at room temperature for 2h. Then add EGDMA10mmol, AIBN0.066g, sonicate for 10min, nitrogen gas for 10min, seal, 60°C, 200r / min shaking incubator to react for 25h. At the end of the reaction, the baicalein molecularly imprinted polymer was first eluted 10 times with methanol-glacial acetic acid (4:1, V / V), washed 5 times with methanol, and then dried in vacuum at 45°C for 24 hours to obtain the baicalein molecularly imprinted polymer. (MIP-3).

[0036] Except that no template molecules were added during the synthesis process, the rest of the steps were the same as those for the preparation of baicalein molecularly imprinted polymers to obtain non-imprinted polymers (NIP-3).

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Abstract

A baicalein molecularly imprinted polymer, a preparation method and application thereof are disclosed. The preparation method comprises: mixing and dissolving baicalein and a functional monomer in a pore forming agent to form a baicalein-functional monomer compound, then adding an effective amount of an initiator and a cross-linking agent, mixing uniformly by ultrasonic, introducing nitrogen into the mixed solution obtained in the above step for sealing; under the protection of nitrogen, employing thermal initiation for the synthesis of the molecularly imprinted polymer, placing in a vibration incubator for a polymerization reaction, after the polymerization reaction is finished, eluting the obtained molecularly imprinted polymer with an organic solvent to remove template molecules, until no template molecules are detected from the eluate, then eluting with methanol to neutrality, and finally performing vacuum drying on the molecularly imprinted polymer to obtain the baicalein molecularly imprinted polymer. Compared with conventional bulk molecularly imprinted polymer, the prepared precipitated molecularly imprinted polymer microsphere has the advantages of good monodispersion, uniform particle size, controllable size, simple preparation, no complex post-processing, short period and the like.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to a method for preparing a molecularly imprinted polymer, in particular to a method for preparing a molecularly imprinted polymer that can specifically recognize baicalein and its metabolites in vivo. Background technique [0002] Scutellaria baicalensis is the dry root of Scutellaria baicalensis Georgi., a plant of the Labiatae family. It has a wide range of pharmacological effects such as anti-inflammatory, anti-viral, anti-allergic, anti-oxidant, and anti-tumor. It is one of the commonly used Chinese medicinal materials in my country. Baicalein is one of the main active ingredients of Scutellaria baicalensis, which has good preventive and therapeutic effects on various diseases including inflammation, tumor, fibrotic disease, cardiovascular and cerebrovascular diseases and neurological diseases. At present, baicalein is mostly extracted by organic solvent extraction or resi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F222/14C08F222/38C08F220/06C08F220/14C08F220/04C08F220/56C08F226/06C08J9/26B01J20/26B01J20/30B01D15/08
Inventor 陈立娜李虹何宏亮高艳坤史丽英都述虎
Owner NANJING MEDICAL UNIV