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Application of Propionibacterium acnes cell-free preparation

A technology of Propionibacterium acnes and cell-free preparations, which is applied in the direction of bacterial antigen components, medical raw materials derived from bacteria, diseases, etc., can solve the problems of cell fragmentation and difficulty in realization, and achieve the reduction of toxic and side effects, no formaldehyde residue, The effect of hypopyrogen

Active Publication Date: 2015-11-25
熊慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many methods for cell disruption, it is difficult to disrupt cells to the nanometer level with uniform particle size
It is technically difficult to process drugs to a particle size of mostly less than 100nm, and studies have shown that nano-scale drugs still have safety risks that are difficult to accurately assess with current technology.

Method used

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  • Application of Propionibacterium acnes cell-free preparation
  • Application of Propionibacterium acnes cell-free preparation
  • Application of Propionibacterium acnes cell-free preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] According to the method described in the 2000 edition of "China Biological Products Regulations", the establishment, verification and other verification of bacterial seed batches were carried out.

[0051] The culture medium adopted in this embodiment is the thioglycolate medium (without agar) produced by Beijing Luqiao Science and Technology Development Company, and its components are: tryptone 15g / l, yeast extract powder 5g / l, glucose 5g / l, Sodium chloride 2.5g / l, L-cystine 0.5g / l, sodium thioglycolate 0.5g / l. The medium was filtered through a 0.22 μm membrane before use.

[0052] Open the PA (scientific name propionibacterium acnes, propionibacterium acnes) 77-1 (see "China Biological Products Regulations") strain tube, each tube contains 6 billion bacteria, and use a capillary to absorb about 0.2-0.3ml of thioglycolate medium ( without agar), add it to the bottom of the strain tube to dissolve it completely, suck it out, add it to the middle tube (capacity 38ml), m...

Embodiment 2

[0057] Referring to the method of Example 1, PA (scientific name propionibacterium acnes, propionibacterium acnes) 65101 (76-27) was inoculated in a nutrient tank after 4 consecutive passages, and was inoculated in a thioglycollate medium (without agar) (before use). The culture medium was filtered with a 0.45 μm membrane), and cultured at 36-37°C for 6 days; microscopically inspected bottle by bottle, and discarded those with contamination by bacteria; combined and collected the cells without bacteria contamination, heated and boiled for 60 minutes, and treated them with ultra-high pressure jets. Collider at 2000kgf / cm 2 Broken bacterium 2 times under pressure; The broken suspension was centrifuged at 8000rpm for 1 hour, and the precipitate was washed 5 times with sterile physiological saline. Pharmacopoeia of the People's Republic of China ") is qualified to make a suspension with a solid content of 2.0mg / ml, and heat at 65°C for 60 minutes to obtain SPAP.

[0058] Detected...

Embodiment 3

[0060] With reference to the method of Example 1, PA (scientific name propionibacterium acnes, propionibacterium acnes) 65102 (H-84) was inoculated in a nutrient tank after 4 consecutive passages, and cultivated in a peptone medium at 36-37° C. for 7 days; Check, those with miscellaneous bacteria contamination are discarded; Merge and collect the thalli without miscellaneous bacteria pollution, heat and boil for 30 minutes, use ultra-high pressure jet collider (Nanonizer Inc. product of Japan, model AQUA300) 2000kgf / cm 2 Broken bacterium 3 times under pressure; The suspended liquid after broken is centrifuged at 12000rpm, washes precipitate 4 times with sterile normal saline, sterility test (2000 version "Chinese Biological Products Regulations") and pyrogen detection (2000 version "People's Republic of China Regulations") Pharmacopoeia ") qualified to make a suspension with a solid content of 1.0mg / ml, heated at 65°C for 100 minutes to obtain SPAP.

[0061] Through the limulu...

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Abstract

The invention relates to an application of a Propionibacterium acne cell-free preparation, and concretely relates to an application of the Propionibacterium acne cell-free preparation in the preparation of medicines for preventing and treating annexitis, cervical erosion, verruca acuminate and leukoplakia vulvae. The Propionibacterium acne cell-free preparation is a cell-free preparation prepared by crushing Propionibacterium acnes and extracting effective components, has a granularity of 10-1000nm, preferably 10-800nm and more preferably 10-500nm, and the Propionibacterium acnes have a number of 76-27, H-84 or 77-1, and preferably 77-1. The pyrogen of the Propionibacterium acne cell-free preparation is less than 320EU / ml, and the Propionibacterium acne cell-free preparation comprises, by mass, 6-60% of proteins, 4-40% of nucleic acids, and the balance polysaccharides. The Propionibacterium acne cell-free preparation has good treatment effects on the annexitis, cervical erosion, verruca acuminate and leukoplakia vulvae.

Description

technical field [0001] The invention relates to an application of Propionibacterium acnes, in particular to the application of a cell-free preparation of Propionibacterium acnes in the preparation of medicines for the prevention and treatment of adnexitis, cervical erosion, condyloma acuminatum and leukoplakia. Background technique [0002] Propionibacterium acnes (propionibacteriumacnes, PA) is derived from the bone marrow of healthy people. It is a Gram-positive anaerobic bacillus. bacteria. Even if the bacterium is isolated from the above-mentioned population, its beneficial immune function to the human body is almost lost. A new type of Propionibacterium acnes cell-free preparation obtained by treating bacteria with ultra-high pressure technology to remove invalid components and extract effective components. As a non-specific immune enhancer, the effect is obvious in animal experiments. Through The strong and lasting stimulating effect on the mononuclear macrophage sys...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/05A61P15/00A61K35/74
Inventor 熊慧
Owner 熊慧
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