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Short corynebacteria pharmaceutics or short corynebacteria pharmaceutics without cells in application for preparing medicine of curing HIV infection or AIDS

A technology of short coryneform bacteria and cell-free preparations, which can be applied in the direction of bacteria, antiviral agents, and pharmaceutical formulations. It can solve the problems of reduced ability, weakened ability to deal with antigens, abnormal B cell function, etc., and achieve improved absorption and diffusion. , Absorption and diffusion are improved, and the particle size is uniform

Inactive Publication Date: 2007-01-10
上海昌润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Macrophages have anti-cytopathic effects caused by HIV infection, but with the continuous replication of the virus, the function of macrophages becomes abnormal, and the ability to process antigens is weakened, which reduces the body's ability to resist HIV infection and other pathogenic infections
[0018] 4. Damaged and dysfunctional B lymphocytes with low levels of CD 4 Molecular expression, but not sure if there is a CCR 5 、CXCR 4 The existence of co-receptors such as HIV, so whether HIV can directly attack B cells is still debated, but it is certain that HIV-infected B cells are abnormal
[0019] 5. Abnormal manifestations of natural killer cell (NK cell) damage. NK cells have immune supervision functions, and have anti-infection and anti-tumor effects. Although the NK cell counts of HIV-infected patients and AIDS patients are normal, they have functional defects and lose supervision to fight against infection and tumors. Anti-tumor cell function
[0025] Although the above-mentioned drugs have shown certain antiviral effects in vivo or in vitro tests and can prolong the survival period of patients, their effects are limited. The toxicity of drugs such as bone marrow suppression and the continuous emergence of drug-resistant virus strains still limit the use of these drugs. Long-term high-dose use, so combination therapy is emphasized at present. Appropriate combination therapy is expected to reduce the dosage of each drug and reduce the frequency of drug-resistant strains

Method used

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  • Short corynebacteria pharmaceutics or short corynebacteria pharmaceutics without cells in application for preparing medicine of curing HIV infection or AIDS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] According to the method described in the 2000 edition of "China Biological Products Regulations", the establishment, verification and other verification of bacterial seed batches were carried out.

[0085] The medium used in this example is the thioglycolate medium (without agar) produced by Beijing Sanyao Science and Technology Development Company, and its ingredients are: tryptone 15g / l, yeast extract powder 5g / l, glucose 5g / l , sodium chloride 2.5g / l, L-cystine 0.5g / l, sodium thioglycolate 0.5g / l. The medium was filtered through a 0.22 μm membrane before use.

[0086] Open the CP (Propionibacterium acnes, scientific name, propionibacterium acnes) 771 (see "China Biological Products Regulations") strain tube, each tube contains 6 billion bacteria, and draw about 0.2-0.3ml of thioglycolate medium (not containing agar), add it to the bottom of the strain tube to dissolve it completely, suck it out, add it to the middle tube (capacity 38ml), mix it with 8-10ml of the ab...

Embodiment 2

[0091] Referring to the method of Example 1, CP (scientific name propionibacterium acnes, propionibacterium acnes) 65101 (76-27) was inoculated in a nutrient tank after 4 consecutive subcultures, and was cultured in a thioglycolate medium (without agar) (before use) Filter the culture medium with a 0.45 μm membrane) and culture at 36-37°C for 6 days; microscopically examine the bottles one by one, and discard those contaminated with bacteria; combine and collect bacteria without bacteria contamination, heat and boil for 60 minutes, and use ultra-high pressure jet Collider at 1500kgf / cm 2 Broken bacterium under pressure 5 times; The broken suspension was centrifuged at 8000rpm for 1 hour, and the precipitate was washed 5 times with sterile physiological saline. Pharmacopoeia of the People's Republic of China ") is qualified to make a suspension with a solid content of 2.0mg / ml, and heat at 65°C for 60 minutes to obtain NCP.

[0092] Detected by Limulus reagent method (2000 edi...

Embodiment 3

[0095] With reference to the method of Example 1, CP (Propionibacterium acnes) 65102 (H-84) was inoculated in a nutrient tank after 4 consecutive passages, and cultured in a peptone medium at 36-37°C for 7 days; bottle by bottle Microscopic examination, those with contamination by miscellaneous bacteria were discarded; combined and collected bacterial cells without contamination by miscellaneous bacteria were heated and boiled for 30 minutes. 2 Broken thalli under pressure 10 times; The suspension after broken is centrifuged at 12000rpm, washes precipitate 4 times with sterile normal saline, sterility test (2000 version "Chinese Biological Products Regulations") and pyrogen detection (2000 version "People's Republic of China Regulations") Pharmacopoeia ") qualified to make a suspension with a solid content of 1.0mg / ml, heated at 65°C for 100 minutes to obtain NCP.

[0096] Detect (solid content 1mg / ml) NCP pyrogen 60EU / ml by limulus reagent method (2000 edition "Pharmacopoeia ...

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Abstract

An application of the CP or NCP preparation in preparing the medicine for treating HIV infection or AIDS is disclosed. Said CP preparation is prepared from CP through deactivating by formaldehyde.

Description

technical field [0001] The invention relates to the application of a short coryneform bacterium preparation (CPP) or a cell-free short coryneform bacterium preparation (NCP) in the field of pharmacy, in particular to the application in the preparation of medicaments for treating HIV infection or AIDS (AIDS), and belongs to the field of medicine. Background technique [0002] AIDS is acquired immunodeficiency syndrome (acquied immunodeficiency syndrome, AIDS), is a serious infectious disease caused by human immunodeficiency virus (human immunodeficiency virus, HIV). Virus specifically invades and depletes lymphocyte differentiation group 4 + T lymphocytes (cluster of differentiation 4 + T cell), that is, CD4 + T cells, also known as T4 cells, cause damage to the body's cellular immunity. Clinically, the initial manifestations are asymptomatic virus carriers, and continue to develop into persistent generalized lymphadenopathy (PGL) and AIDS related complex (ARC), with fever...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/05A61P31/18C12N1/20A61K35/741
Inventor 高尚先李守悌
Owner 上海昌润生物科技有限公司
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