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Streptomyces lavendulae and application of Streptomyces lavendulae to preparation of algae microbial fertilizer

A technology of Streptomyces lavender and microbial fertilizers, applied in the field of microorganisms, can solve the problems of low recognition by farmers, lack of nutrition, and slow action, and achieve outstanding degradation effects, low prices, and growth-promoting effects

Active Publication Date: 2014-01-29
青岛藻源植物营养有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The main disadvantage of the above-mentioned seaweed residue fertilizer is that the degradation effect of seaweed residue is poor during the decomposing process, and most of the microorganisms used are broad-spectrum strains, which cannot specifically degrade seaweed residue. Aspergillus, etc. can also cause fungal diseases such as plant soft rot
In addition, this type of seaweed residue fertilizer is mostly fermented by microorganisms and then mixed with chemical fertilizer raw materials. Although it has a certain fertilizer effect, most of the effective bacteria in it are dead and cannot play their due role at all. Due to the lack of nutrients, microbial fermentation products have more effective bacteria, but they are slow to function, low in farmers' recognition, and difficult to promote and apply in the market

Method used

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  • Streptomyces lavendulae and application of Streptomyces lavendulae to preparation of algae microbial fertilizer
  • Streptomyces lavendulae and application of Streptomyces lavendulae to preparation of algae microbial fertilizer
  • Streptomyces lavendulae and application of Streptomyces lavendulae to preparation of algae microbial fertilizer

Examples

Experimental program
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Effect test

Embodiment 1

[0065] (1) Seaweed residue pretreatment

[0066] Take the fresh seaweed residue produced after seaweed processing, measure its water content, and adjust its water content to about 55%-60% by water replenishment or drying.

[0067] (2) Preparation of fermentation medium

[0068] Take 500kg of seaweed residue prepared in step (1), add 150kg of bran (30%, W / W), 50kg of rice husk (10%, W / W), 5kg of potassium nitrate (1%, W / W), 10kg Soluble starch (2%, W / W), KH 2 PO 4 (0.1%, W / W) 0.5kg, MgSO 4 (0.01%, W / W) 0.05kg, the pH is adjusted with ammonia water, stirring continuously in the mixing equipment, the material is required to be moist but not agglomerated until it is uniform and loose, and the pH is stable at 7.5.

[0069] (3) Medium packing and sterilization

[0070] Evenly divide the culture medium obtained in step (2) into special shallow trays for fermentation, with a thickness of 5cm, and cover the surface with six layers of gauze, sterilize with high-pressure steam at 12...

Embodiment 2

[0084] Get the seaweed microbial fertilizer of embodiment 1 gained, measure its antibacterial activity to several common plant pathogenic fungi.

[0085] (1) Pathogen strains tested

[0086] Rhizoctonia solani, Fusarium oxysporum, Fusarium solani, Fusarium moniliforme, Apple black solanum, Wheat root rot, Corn leaf spot, Gramines anthracnose;

[0087] (2) Determination of the antibacterial activity of live strains against pathogenic fungi

[0088] Take 10 g of the seaweed microbial fertilizer obtained in Example 1, add it into 100 ml of sterile water and shake it evenly under aseptic conditions to make a bacterial suspension containing Streptomyces lavender gray (CGMCC No.7548).

[0089] Draw a vertical cross on the prepared PDA plate with the origin as the center, use an inoculation loop to inoculate the bacterial suspension along one line under aseptic conditions, and connect the two ends of the other line with the same distance from the center as in step (1). Describe tes...

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Abstract

The invention relates to a Streptomyces lavendulae strain, which is named as Streptomyces lavendulae in classification. The Streptomyces lavendulae strain is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.7548. The invention also relates to application of the Streptomyces lavendulae to preparation of algae microbial fertilizer. The Streptomyces lavendulae strain provided by the invention has a prominent degrading effect on algae residues; waste residues generated after seaweed gel is extracted by the algae processing industry can be recycled, and antibiotic metabolic active substances generated in the fermenting process of the Streptomyces lavendulae can effectively inhibit growth of various pathogenic microorganisms; the Streptomyces lavendulae has excellent effects of promoting growth and increasing yield and has an obvious effect in agricultural production.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Streptomyces lilacaceae strain and its application in preparing seaweed microbial fertilizers from fermenting seaweed residue. Background technique [0002] So far, seaweed has shown great development potential and economic value in the fields and scope of food, marine medicine, animal feed, organic fertilizer, cosmetics and bioenergy. During the processing and utilization of seaweed, a large amount of waste will be produced - seaweed residue, which is rich in alginic acid, mannitol, betaine, chitosan, polyphenols and seaweed fiber, pectin, protein, Substances such as lignin. Therefore, how to further process and utilize seaweed slag and maximize its value has become a problem of increasing concern. [0003] Chinese invention patent CN101439997A discloses a preparation method of seaweed organic fertilizer, which uses the waste residue after seaweed gel to be fermented to pro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/465
Inventor 赵宏涛李丽王鹏李园园公春艳
Owner 青岛藻源植物营养有限公司
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