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Folic acid-porous platinum-graphene oxide composite nanomaterials and its detection of tumor cells

A graphene composite and nanomaterial technology, applied in the fields of nanotechnology and medical testing, can solve problems such as difficulty in achieving detection effects, and achieve the effects of good peroxidase activity and rapid and sensitive detection

Inactive Publication Date: 2016-02-17
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional detection and localization of tumors is difficult to achieve the expected detection effect due to the lack of methods to achieve targeted positioning

Method used

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  • Folic acid-porous platinum-graphene oxide composite nanomaterials and its detection of tumor cells
  • Folic acid-porous platinum-graphene oxide composite nanomaterials and its detection of tumor cells
  • Folic acid-porous platinum-graphene oxide composite nanomaterials and its detection of tumor cells

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0039] Weigh 1.2 g of 325 mesh flake graphite, and add it to a mixed solution of 144 mL of concentrated sulfuric acid with a concentration of 18 mol / L and 16 mL of phosphoric acid with a concentration of 15 mol / L. After stirring well, place in an ice bath at 0 °C. Slowly add 7.2g of potassium permanganate in small amounts to the above mixed solution several times. After keeping the ice bath at 0°C and magnetic stirring for 3 hours, the obtained dark green suspension was transferred to a warm water bath at 50°C, and the temperature was controlled for 12 hours. The obtained purple-black suspension was slowly added into 160 mL of ice-water mixture, and stirred vigorously for 1 hour. Then, 4 mL of 30% (mass concentration) hydrogen peroxide solution was added dropwise to the solution, and the color of the solution changed to bright yellow. The resulting solution was filtered through a G1 sand core funnel (pore size 20-30 microns), the filtrate was centrifuged at 4000 rpm for 30 m...

example 2

[0041] 160 mg of polyallylamine hydrochloride and 160 mg of potassium hydroxide were sequentially added to 80 mL of 0.5 mg / mL graphene oxide aqueous solution, and the mixture was magnetically stirred in a water bath at 70°C for 24 hours. The solution was collected and then centrifuged, washed with double distilled water until neutral, and excess polyallylamine hydrochloride and potassium hydroxide were removed. The obtained product was made up to 80 mL with double distilled water and fully dissolved.

example 3

[0043] 40 mg of folic acid was first dissolved in 16 mL of sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution (pH5, 20 mmol / L), and the 0.0227 mmol / L folic acid solution obtained after complete dissolution was mixed with 8 mL of 1-ethyl-3-(3 -Dimethylaminopropyl)-carbodiimide aqueous solution (0.068mmol / L) and N-hydroxysuccinimide (0.068mmol / L) mixed aqueous solution were mixed, and activated at room temperature overnight in the dark. Then fully mixed with the 80 mL polyallylamine hydrochloride modified graphene oxide aqueous solution obtained in Example 2, and magnetically stirred at room temperature for 24 hours. After the reaction, the solution was collected and centrifuged by ultrafiltration, washed with double distilled water until neutral, and unbound folic acid and unreacted cross-linking agent were removed. The obtained product was made up to 80 mL with double distilled water, and fully dissolved to obtain a folic acid-graphene oxide aqueous soluti...

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Abstract

The invention discloses a folic acid - porous platinum - Graphene oxide composite nanomaterials and its detection of tumor cells , which is folic acid - porous platinum - Graphene Oxide Composite Nanomaterials Using graphene oxide as the substrate, folic acid molecules were cross-linked by amidation reaction, and platinum nanoparticles with porous structure were synthesized in situ to prepare a folic acid-porous platinum-graphene oxide composite nanomaterial. Folic acid-porous platinum-graphene oxide composite nanomaterials can specifically recognize folate receptors on the cell surface, and at the same time have the characteristics of mimicking peroxidase, which can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine by hydrogen peroxide Hydrochloride color development. The color reaction can be used to quickly and sensitively detect the number of tumor cells, and the minimum detection rate can be as low as 30 MCF-7 cells. It can also catalyze diaminobenzidine to produce a reddish-brown precipitate on the cell surface for targeted detection.

Description

technical field [0001] The invention relates to the preparation of folic acid-porous platinum-graphene oxide composite nanomaterials and a method for rapidly and sensitively detecting tumor cells, belonging to the fields of nanotechnology and medical testing. Background technique [0002] Despite advances in medical technology, cancer remains the deadliest disease in the world. The traditional detection and localization of tumors is difficult to achieve the expected detection effect due to the lack of methods to achieve targeted localization. The development of new methods for early diagnosis of cancer is critical to successfully treating cancer and increasing patient survival. [0003] Folic acid is a small molecular vitamin. Since scientists discovered in 1986 that folic acid enters cells through the receptor-mediated endocytic pathway, people have begun to conduct in-depth research on folic acid as a targeting carrier. It has been confirmed that folic acid receptors are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31G01N33/574G01N33/558
Inventor 陈伟张灵娜邓豪华许雄伟李光文
Owner FUJIAN MEDICAL UNIV
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