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Novel strain for producing butanol and method for producing butanol

A technology of butanol and strains, applied in the field of butanol-producing strains, can solve problems such as inability to use fermentation to produce butanol, and achieve the effects of great social significance and economic value

Active Publication Date: 2014-02-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Annous et al. (Appl.Environ.Microbiol. 1991, 57: 2544-2548) used chemical mutagenesis to obtain the super strain BA101, and the total solvent in a single batch of fermentation could reach more than 25 g / L; Thaddeus Ezeji et al. (Bioresource Technology. 2008 , 99: 5915-5922) using the mutant strain BA101 to ferment the acid hydrolyzed and enzymatically hydrolyzed sugar liquid of corn cobs detoxified with XAD-4 resin as the substrate, the total solvent yield was 9.30 g / L, but it could not utilize the non-detoxified Acid hydrolysis and enzymatic hydrolysis of sugar liquid fermentation to produce butanol

Method used

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  • Novel strain for producing butanol and method for producing butanol
  • Novel strain for producing butanol and method for producing butanol
  • Novel strain for producing butanol and method for producing butanol

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1: This example illustrates the method for screening Clostridium beinii IT66 by mutagenesis using the original strain IB4 of Clostridium beinii as the starting strain.

[0041] The source of the starting strain: the strain disclosed in the previous literature of the applicant's inventor team, Guo et al. (J Ind Microbiol Biotechnol, 2011, 99 (3), 401-407.). The applicant hereby declares that it guarantees to distribute the biological materials of this starting strain to the public free of charge within 20 years from the date of application.

[0042] The medium formula used in this embodiment (% is mass percentage):

[0043] (1) Solid plate medium: yeast powder 0.3%, peptone 0.5%, soluble starch 1%, ammonium acetate 0.2%, sodium chloride 0.2%, magnesium sulfate heptahydrate 0.3%, potassium dihydrogen phosphate 0.1%, dihydrogen phosphate Potassium 0.1%, ferrous sulfate heptahydrate 0.01%, agar 1.5%, the rest is water, pH 6.

[0044] (2) Resazurin plate medium: ...

Embodiment 2

[0065] Embodiment 2: This embodiment illustrates the method that Clostridium beinii IT66 produces butanol

[0066] The medium formula used in this embodiment:

[0067] Plate medium: yeast powder 0.3%, peptone 0.5%, soluble starch 1%, ammonium acetate 0.2%, sodium chloride 0.2%, magnesium sulfate heptahydrate 0.3%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, Ferrous sulfate heptahydrate 0.01%, agar powder 1.5%, the rest is water, pH 6.

[0068] Seed medium: yeast powder 0.3%, peptone 0.5%, soluble starch 1%, ammonium acetate 0.2%, sodium chloride 0.2%, magnesium sulfate heptahydrate 0.3%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, Ferrous sulfate heptahydrate 0.01%, the rest is water, pH 6.

[0069] Fermentation medium: non-detoxified corncob acid hydrolysis sugar solution 3%; ammonium acetate 0.22%, potassium dihydrogen phosphate 0.05%, dipotassium hydrogen phosphate 0.05%, magnesium sulfate heptahydrate 0.02%, m...

Embodiment 3

[0076] Embodiment 3: This embodiment illustrates the method that Clostridium beinii IT66 produces butanol

[0077] The medium formula used in this embodiment:

[0078] Plate medium: yeast powder 0.3%, peptone 0.5%, soluble starch 1%, ammonium acetate 0.2%, sodium chloride 0.2%, magnesium sulfate heptahydrate 0.3%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, Ferrous sulfate heptahydrate 0.01%, agar powder 1.5%, the rest is water, pH 6.

[0079] Seed medium: yeast powder 0.3%, peptone 0.5%, soluble starch 1%, ammonium acetate 0.2%, sodium chloride 0.2%, magnesium sulfate heptahydrate 0.3%, potassium dihydrogen phosphate 0.1%, dipotassium hydrogen phosphate 0.1%, Ferrous sulfate heptahydrate 0.01%, the rest is water, pH 6.

[0080] Fermentation medium: non-detoxified corncob acid hydrolyzed sugar solution 3.5%; ammonium acetate 0.22%, potassium dihydrogen phosphate 0.05%, dipotassium hydrogen phosphate 0.05%, magnesium sulfate heptahydrate 0.02%,...

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Abstract

The invention discloses a novel clostridium beijerinckii strain for producing butanol. The strain has a classification name of clostridium beijerinckii IT66 (Clostridium beijerinckii IT66) and has a collection number of CCTCCM NO:2011404. The invention also discloses application of the clostridium beijerinckii in fermentation production of the butanol. The clostridium beijerinckii IB4 is subjected to continuous culture and plasma induced mutation by adopting a chemostat, and a strain which tolerates an inhibitor and is high in reducing power is screened through a resazurin flat plate and is high in acidolysis byproduct resistance, high in butanol ratio and high in solvent yield. When a non-detoxified corncob acidolysis sugar solution is a carbon source, the total solvent yield and butanol yield in a 5L fermentation tank respectively reach 11.3g / L and 9.1g / L and are respectively improved by 2.3 times and 2.4 times compared with those of an original strain cultured under the same conditions, the butanol ratio is 80 percent, the sugar conversion rate is 0.32, and the strain has significant social meaning and economic value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a new butanol-producing bacterial strain and a method for producing butanol. Background technique [0002] With the accelerated depletion of petroleum resources and the soaring price, the production of butanol by fermentation has received extensive attention and has become one of the research hotspots of bioenergy. As a new generation of liquid energy, butanol is being valued by more and more countries. It has the advantages of high energy density, direct use in internal combustion engines, and convenient transportation. In today's increasingly severe energy crisis, butanol has broad potential as a fuel. development prospects. [0003] Clostridium acetobutylicum and Clostridium beinii can directly and effectively utilize starch substances such as corn or molasses and other raw materials, and the butanol-producing strains have high utilization efficiency of glucose and xylose in fibe...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/16C12R1/145
Inventor 姜岷贺爱永尹春燕孔祥平陈佳楠马江锋韦萍
Owner NANJING UNIV OF TECH
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